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461.
In this article, we present a microfluidic platform for passive fluid pumping for pump-free perfusion cell culture, cell-based assay, and chemical applications. By adapting the passive membrane-controlled pumping principle from the previously developed perfusion microplate, which utilizes a combination of hydrostatic pressure generated by different liquid levels in the wells and fluid wicking through narrow strips of a porous membrane connecting the wells to generate fluid flow, a series of pump-free membrane-controlled perfusion microfluidic devices was developed and their use for pump-free perfusion cell culture and cell-based assays was demonstrated. Each pump-free membrane-controlled perfusion microfluidic device comprises at least three basic components: an open well for generating fluid flow, a micron-sized deep chamber/channel for cell culture or for fluid connection, and a wettable porous membrane for controlling the fluid flow. Each component is fluidically connected either by the porous membrane or by the micron-sized deep chamber/channel. By adapting and incorporating the passive membrane-controlled pumping principle into microfluidic devices, all the benefits of microfluidic technologies, such as small sample volumes, fast and efficient fluid exchanges, and fluid properties at the micro-scale, can be fully taken advantage of with this pump-free membrane-controlled perfusion microfluidic platform. 相似文献
462.
通过深入调研广东、福建和江苏等校地共建新型研发机构,运用扎根理论方法提取影响新型研发机构创新成效的核心因素,包括地方政府创新条件、母体高校创新条件、创新能力、院校合作及双重创新网络特征;通过基于模糊集的定性比较分析,得到导致新型研发机构高创新成效的组态路径构型.研究发现:技术创新网络中心性、创新能力和院校合作是影响校地共建新型研发机构创新成效的必要条件,且其均通过创新网络发挥影响作用.最后,对校地共建新型研发机构的创新发展提出鼓励与母体高校开展产学研合作、引导与区域高校和产业合作、激励实施成果转化和管理制度现代化、设立研究基金和促进投资主体多元化,以及灵活化组织体制、市场化运行机制等对策建议. 相似文献
463.
The deep economic, social, technological and cultural changes in Europe represent a real challenge for teacher education. The teacher's role is becoming more complex and demanding, while the expectations of society are rising. Teachers are expected not only to enable optimal development of increasingly heterogeneous groups of pupils, but also to mitigate the effects of social inequality. All this requires qualitative changes in pre-service and in-service teacher education. In this regard, the transition from school/university to professional life — the induction period and early career years — requires special attention. In this phase, a fruitful synthesis of theory and practice can occur if novices are systematically introduced and supported by good mentoring in the transition from study to school life and culture, but a ‘practice shock’ is also possible during which many positive effects of pre-service education are lost. Our aim is to identify some principles and optimal solutions that would foster teacher's professional development in this important phase of their career. 相似文献
464.
刘坡 《柳州职业技术学院学报》2010,10(1):75-77
拟古的新打油诗《我的失恋》一改鲁迅往日的严肃,用打油诗的方式戏谑地显示了大师敏锐的眼力,“我的失恋”并不是心伤,而是胜利后的一声冷笑,嘲讽那些无聊的失恋诗,盲目的爱情,让无知的青年在蹙眉一笑后警醒,故而这是一场胜利的失恋。 相似文献
465.
OBJECTIVES: To evaluate whether or not the original six-factor structure of the Child Abuse Potential (CAP) Inventory suggested by [Milner, J.S. (1986). The Child Abuse Potential Inventory: Manual (2nd ed.). DeKalb, IL: Psytec. Inc.] can be confirmed with data from a group of Chinese mothers in Hong Kong. METHOD: Eight hundred and ninety-seven mothers from 13 child care centers in Hong Kong successfully completed a set of structured questionnaires consisting, among others, of the 77-item Abuse Scale of the CAP Inventory. A confirmatory factor analysis (CFA) was performed to determine whether the data fits the factorial structure of the CAP Inventory identified by Milner (1986). RESULTS: The root mean square error of approximation (RMSEA) of the model epsilona) is 0.031, indicating a close fit of the model in relation to the degrees of freedom. On testing the close fit against the null hypothesis that Ho: epsilona相似文献
466.