乳腺癌耐受蛋白介导5-氟脲嘧啶的耐受及机制探讨

AIM To filtrate breast cancer resistance protein(BCRP)-mediated resistance agents and investigate the mechanism,so as to provide valuable datum for optimization clinical chemotherapy scheme to tumor with evaluation marker of BCRP expression. METHODS MTT assay was used to filtrate BCRP-mediated resistance agents with PA317/Tet-on/TRE-BCRP cell of different expression levels of BCRP after treated with different concentration anticancer agents. High performance liquid chromatography(HPLC) was applied to measure relative dose of intracellular retention resistance agents. Nuclear DNA fluorescence dye,Hochest 33258, staining and flow cytometry were adopted to detect apoptotic cells after treated with drugs. RESULTS There were shown increasing durg-resistance to 5-fluorouracil,methotrexate, doxirubicin, pirarubicin,etoposide and mitoxantrone followed with increasing expression of BCRP on PA317/Tet-on/TRE-BCRP cells(P＜0.05, n=3),but shown sensitive to paclitaxel, cisplatin, vincristine, mitomycin and vindesine. There also was shown significant negative correlation between the intracellular retention dose of 5-fluorouracil with different expression of BCRP(r=-0.885, P＜0.05, n=3).There were shown parallel results of that decreasing cellular apoptotic rate with increasing cellular expression of BCRP after treated with 5-fluorouracil by fluorescence dye staining and flow cytometry(P＜0.05, n=3),and also shown significate rise of the apoptotic rate of BCRP expression cells after treated with Ko143 (P＜0.05, n=3). Every group of cells could be different extently blocked in phase of G0/G1 treated with 5-fluorouracil. CONCLUSION Resistance of 5-fluorouracil could be especially mediated by conjugated with BCRP and acted as drug exclude-pump substrate. Cellular ability resistant to 5-fluorouracil-induced apoptosis could be reinforced by BCRP expression.     

数列知识在化学解题中的应用

将化学问题抽象成为数学问题，利用数学工具、结合化学知识通过计算和推理解决化学问题的能力，是高考考查考生思维能力的一个重要方面，《考试说明》对此也做了明确的说明，将化学问题中所给信息进行抽象归纳、逻辑地统摄成规律，转化成数学问题进行教学，能有效提高学生的逻辑思维能力和学科之间的综合能力，本文就数列知识在解决化学试题中的运用做一些讨论。     

建筑隔震橡胶支座的安装质量控制

本文介绍了在某项施工过程中，如何有效控制隔震橡胶支座的安装质量问题。     

关于推进民族地区双语教师继续教育的若干思考

推进民族地区双语教师继续教育要坚持以政府组织实施为主导,立足于基础教育的实际,明确对象、目标和要求,规划继续教育的蓝图;强化统一领导、分级负责、分类管理,建立纵向层级管理与横向自我调节相结合的管理体制;经费要列入财政预算,并与普通教育经费的三个增长实现同步配套核拨;实践中要以强化管理、提高质量和整体效益为主要目标;防止和克服教材与课程的逻辑关系倒置,教材内容要着重体现中华民族持久、强大的凝聚力,体现语言与专业知识的有机融合.     

从关联理论看阅读理解的心理过程

试图从关联理论的角度，讨论阅读理解的心理过程，以及在此过程中影响阅读理解能力提高的主要因素，并提出关联理论在阅读教学中的实践意义，即在阅读课中，有助于教师改进教学方法，提高教学质量；有助于学生准确把握作意图，提高阅读理解的准确率和速度。     

新课程理念下开展STSE教育的思考

STS教育和情感(Emotion)教育是当今物理新课程特别强调的两个重要方面,二者的结合,即STSE教育是教育者需要研究的重大课题。本文从物理教学中开展STSE教育的必要性出发,论述了STSE教育的理论基础———和谐教育场的构建,提出了开展STSE教育的四条途径,讨论了STSE教育思想和新课程评价方式的相关性。     

利用课堂教学培养学生创新能力

利用课堂教学，从营造民主和谐的教学氛围，师生双方教、学观念的更新以及开放性教学三方面来培养学生的创新能力。     

冷核聚变和新物理(英文)

本文阐明了揭示新物理过程的冷核聚变及达到这一过程和激发能的条件     

高职院校产学合作教育创新系统体系的构建

高职院校产学合作教育是高等职业教育改革与发展的战略主攻方向,也是提高自主创新能力的重要途径之一。在分析创新系统、高职院校产学合作教育的一般构成和理论研究取向的基础上,提出了中国高职院校教育创新系统的构成及构建思路。     

How to sustainably increase students’ willingness to protect pollinators

The current loss of biodiversity requires efforts to increase awareness of pollinator conservation. An important tool is education which often uses the honeybee (Apis mellifera) as an exemplary organism to reach this goal. Any successful module needs to focus on reducing the perceived danger associated with fear, in order to support the willingness to protect them. Using a quasi-experimental design, we investigated the effectiveness of two educational approaches: one by authentically encountering living animals at a beehive, the other by using a remote online beehive. We monitored secondary school students’ (N = 354) perception of bees with respect to interest, danger and conservation as well as situational emotions (interest, well-being, boredom) during both interventions. In both cases positive effects on perception levels were observed, even when already a high willingness to protect bees existed. Using living animals in educational settings is crucial, especially when students’ situational emotions need targeting. However, we achieved similar intervention results in perception levels using a remote beehive, which therefore constitutes an excellent alternative to raise awareness of the conservation of bees as pollinators when working with living organisms is not possible.