生活方式变迁中溱潼会船民俗的困境与抉择

运用田野调查法、文献资料法等方法,对江苏溱潼农村居民的生活方式变迁历程进行了梳理,并从劳动生活方式、消费生活方式、闲暇生活方式、交往生活方式、家庭婚姻生活方式等五个方面就溱潼会船民俗所面临的困境展开了剖析。研究结果认为：伴随着溱潼地区现代化、城市化进程的不断加快,孕育传统会船民俗的社会土壤也在不断的流失,曾经在溱潼地方社会中发挥重要整合、凝聚功能的会船民俗,在生活方式变迁的进程中遭遇到了诸多困境与不适,据此提出对策与建议,以供相关政府部门决策参考。     

Biofunctionalized nanoslits for wash-free and spatially resolved real-time sensing with full target capture

We propose biofunctionalized nanofluidic slits (nanoslits) as an effective platform for real-time fluorescence-based biosensing in a reaction-limited regime with optimized target capture efficiency. This is achieved by the drastic reduction of the diffusion length, thereby a boosted collision frequency between the target analytes and the sensor, and the size reduction of the sensing element down to the channel height comparable to the depletion layer caused by the reaction. Hybridization experiments conducted in DNA-functionalized nanoslits demonstrate the analyte depletion and the wash-free detection ∼10 times faster compared to the best microfluidic sensing platforms. The signal to background fluorescence ratio is drastically increased at lower target concentrations, in favor of low-copy number analyte analysis. Experimental and simulation results further show that biofunctionalized nanoslits provide a simple means to study reaction kinetics at the single-pixel level using conventional fluorescence microscopy with reduced optical depth.     

Microfluidic chemical processing with on-chip washing by deterministic lateral displacement arrays with separator walls

We describe a microfluidic device for on-chip chemical processing, such as staining, and subsequent washing of cells. The paper introduces “separator walls” to increase the on-chip incubation time and to improve the quality of washing. Cells of interest are concentrated into a treatment stream of chemical reagents at the first separator wall for extended on-chip incubation without causing excess contamination at the output due to diffusion of the unreacted treatment chemicals, and then are directed to the washing stream before final collections. The second separator wall further reduces the output contamination from diffusion to the washing stream. With this approach, we demonstrate on-chip leukocyte staining with Rhodamine 6G and washing. The results suggest that other conventional biological and analytical processes could be replaced by the proposed device.     

体育硕士研究生教学能力现状与培养策略研究

研究生教育是高等教育的重要组成部分,文章主要采用文献资料法、问卷调查法、专家访谈法对东北师范大学体育硕士研究生教学能力的基本现状进行调查,研究结果显示:东北师范大学体育学科建设合理,基础教育特色鲜明,体育硕士研究生在组织能力、传授能力、创编能力、评价能力方面相对较好,有利于对体育硕士研究生的培养;建议加强和完善导师责任制,强化校内外导师组联合培养制,完善教师梯队的建设,从多层面对体育硕士研究生进行培养,注重体育研究生综合能力的提升,以期全面提升体育硕士的教学能力,培养卓越的人民体育教师。     

外国文学知识引入大学英语课堂的可行性分析

高校教改的深入要求大学英语课堂灵活应对,摆脱僵化的课堂模式。外国文学知识的引入有助于提高学生综合素质及培养适应全球化发展的国际化人才,因此在大学英语课堂中具有重要的实践意义。本文也列举出了将外国文学知识引入课堂的有效方式来探讨其可行性。     

Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation

Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since they may provide a better model of the tumor than conventional monolayer culture. Moreover, tumor cell interaction with the extracellular matrix can determine cell organization and behavior. In this work, a microfluidic system was used to form cell-laden core-shell beads which incorporate elements of the extracellular matrix and support the formation of multicellular spheroids. The bead core (comprising a mixture of alginate, collagen, and reconstituted basement membrane, with gelation by temperature control) and shell (comprising alginate hydrogel, with gelation by ionic crosslinking) were simultaneously formed through flow focusing using a cooled flow path into the microfluidic chip. During droplet gelation, the alginate acts as a fast-gelling shell which aids in preventing droplet coalescence and in maintaining spherical droplet geometry during the slower gelation of the collagen and reconstituted basement membrane components as the beads warm up. After droplet gelation, the encapsulated MCF-7 cells proliferated to form uniform spheroids when the beads contained all three components: alginate, collagen, and reconstituted basement membrane. The dose-dependent response of the MCF-7 cell tumor spheroids to two anticancer drugs, docetaxel and tamoxifen, was compared to conventional monolayer culture.     

谈“四层次、五契合”高技能人才培养模式的构建与实践

天津职业技术师范大学附属技师学院通过多年来的教学实践和研究,结合天津地区现代工业技术发展的特点,秉承"半工半读"历史文化传统,积极推进人才培养模式创新,发挥校企资源优势,依托"校企合一"的现实办学环境,以工学结合为核心,构建了基于校内企业的产教融合实践教学平台,系统确立并实施了"四层次、五契合"人才培养模式,凸显了在高技能人才培养领域的示范引领作用。     

“男子气概”与“女性气质”的动荡融合——《红楼梦》中贾宝玉性别的二元性解读

《红楼梦》文本通过各种各样的方式强化了作为两性区分特征的"男子气概"与"女性气质"的概念。采用性别的视角对文本进行解读,探究贾宝玉的性别危机——男子气概与女性气质的动荡融合,以审视宝玉所面临的性别抉择危机,揭示小说文本对男性凌驾于女性之上的传统等级制度的二元对立进行强化的多种方式。     

视野的更新:论技术发展对体育传播领域的冲击(英文)

自古希腊时期的第一届奥林匹克运动至当下的电子竞技体育,媒体技术的发展已经非常成熟。体育自身发生了许多变化,指导体育事业的思想观念也发生了天翻地覆的变化。体育传播亦是,也发生了许多变化,进行了诸多革新。     

提高科技期刊校对质量的几点措施

科技期刊的校对工作是编辑工作的继续,具有对编辑工作实施监督、检查、补充和完善的多种功能。校对的质量直接影响期刊的质量。校对工作是出版工作中一个不可缺少的环节,是保证期刊质量的重要措施之一。阐述了校对中常见的错误,提出了提高校对质量的几点措施。