Isolation and manipulation of living adherent cells by micromolded magnetic rafts

A new strategy for magnetically manipulating and isolating adherent cells with extremely high post-collection purity and viability is reported. Micromolded magnetic elements (termed microrafts) were fabricated in an array format and used as culture surfaces and carriers for living, adherent cells. A poly(styrene-co-acrylic acid) polymer containing well dispersed magnetic nanoparticles was developed for creating the microstructures by molding. Nanoparticles of γFe(2)O(3) at concentrations up to 1% wt.∕wt. could be used to fabricate microrafts that were optically transparent, highly magnetic, biocompatible, and minimally fluorescent. To prevent cellular uptake of nanoparticles from the magnetic polymer, a poly(styrene-co-acrylic acid) layer lacking γFe(2)O(3) nanoparticles was placed over the initial magnetic microraft layer to prevent cellular uptake of the γFe(2)O(3) during culture. The microraft surface geometry and physical properties were altered by varying the polymer concentration or layering different polymers during fabrication. Cells plated on the magnetic microrafts were visualized using standard imaging techniques including brightfield, epifluorescence, and confocal microscopy. Magnetic microrafts possessing cells of interest were dislodged from the array and efficiently collected with an external magnet. To demonstrate the feasibility of cell isolation using the magnetic microrafts, a mixed population of wild-type cells and cells stably transfected with a fluorescent protein was plated onto an array. Microrafts possessing single, fluorescent cells were released from the array and magnetically collected. A post-sorting single-cell cloning rate of 92% and a purity of 100% were attained.     

个性化教育的研究与实践

对学校后勤人员的考核方法，一般来讲有三种：一种是定性考核，一种是定量考核，一种是两的结合。我们提出的量化测评办法，是指对被考核所设置的考核指标，除无法量化的少部分内容采用否决限制外，使定性考核所得的等级转化为定量化，从而与定量考核融为一体，使被考核的工作业绩明晰而确定。量化测评办法在考核学校后勤人员工作中的运用，意在使考核工作更具简洁性、可操作性、现实性和科学性。     

大众文化对高校思想政治教育的影响研究

大众文化对高校思想政治教育的影响具有双重性,既有拓展教育载体、革新教育方法、丰富教育资源的积极影响;又有消解思想政治教育主导理论形态,抑止大学生自由而全面发展,销蚀高校思想政治教育文化环境,造成学生理想信念缺失的消极影响.应该通过开展高校马克思主义大众化研究,改革思想政治理论课教学模式,营造先进的校园文化环境强化高校思想政治教育.     

Using Rogers's Diffusion of Innovation Model to Examine the Willingness to Pay for Public Television in Taiwan

This study adopts Rogers's diffusion of innovation model to examine the factors affecting the willingness of the Taiwanese to pay a license fee to watch public television. A telephone survey was used, and 506 valid questionnaires were obtained, representing a response rate of 58.6%. The data analysis showed that most findings of this study were not congruent with the prediction of Rogers's model, indicating that the Taiwanese do not consider paying a license fee a feasible means to support public television. However, this study found that the frequency of public television viewing was the most powerful factor positively affecting people's intention to pay for public television.     

湘南地区农村居民收入与消费问题的调查研究

通过实地走访和问卷调查,并以湘潭市的数据作为参照,对比分析了湘南地区农民收入来源和消费结构现状;针对湘南地区农村居民收入与消费存在的主要问题以及消费的影响因素,从提高农民收入、提升人力资本水平、改善农村消费环境和基础设施、构筑与完善农村现代金融服务体系几个方面提出了解决途径。     

明治时期日本人眼中的李鸿章

明治时期,日本人眼中的李鸿章纵使再有才华,不过是腐朽、破败清朝的代表而已。不过,明治时期,关于李鸿章的著作在日本地大量出现,说明了李鸿章在当时所受到的关注程度之高。     

韦伯斯特与美国研究图书馆协会

作为美国研究图书馆协会(ARL)前主席,韦伯斯特是ARL的重要掌舵人。 20年来,他和ARL其他成员一起,推动建立图书馆版权联盟,推进美国著作权立法议程;参与建立网络信息联盟,探讨互联网理论框架的建构;推动成立学术出版和学术资源联盟,在全球范围内创建学术出版的新模式。同时,韦伯斯特注重培养评估文化,领导并推动开发图书馆评估StatsQUAL工具套件。韦伯斯特带领下的ARL对美国的图书馆、学术交流以及公共政策产生了强大而持久的影响。     

如何高效复习，应对历史中考开卷考试

历史开卷考试是体现新课程教学理念的变化,体现以创新精神和实践能力为重点的素质教育要求,减轻学生不必要负担的考试形式。这种考试不是打开课本照抄照搬书上的知识点,简单地罗列知识点,而是通过显性知识透视其隐性联系,它需要以基础知识为载体,能力立意和知识能力相融合,强调学以致用,以丰富的想像力和迁移能力对固有知识体系进行重新整合。     

华南理工大学工程训练中心建设的实践与探索

主要就工程训练中心的教学体系和内容、教学方法和手段以及基地建设等方面进行了探讨,形成了"三大类"、"八大模块"的工程训练教学体系、固定训练模式和开放训练模式相结合的训练方法,同时构建了面上创新、重点创新和综合创新的"三创"体系,最终建立起特色鲜明、理念先进的现代工程训练基地,全面提高学生综合素质.     

从“无意识”到“血性意识”——论劳伦斯对弗洛伊德学说的继承与超越

D.H.劳伦斯与西格蒙德·弗洛伊德都致力于探索人类心灵的无意识领域,强调性欲在人本性中的作用.本文探讨劳伦斯与弗洛伊德学说的承继关系,指出劳伦斯的一些见解虽受惠于弗洛伊德,但劳伦斯是一位具有独创性的文学家,他的思想远比弗洛伊德的激进.