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71.
72.
Papi Mostafa Bondarenko Anna Vitalyevna Wawire Brenda Jiang Chen Zhou Shiyao 《Reading and writing》2020,33(2):507-509
Reading and Writing - The original publication of the article contained errors in the tables. 相似文献
73.
The historic town of Huangyao is located in the eastern part of southwest Guangxi Zhuang Autonomous Region at the lower stream of the Lijiang River. The town took shape in 968 and thrived during the 17th century. 相似文献
74.
Intracellular drug delivery by rapid squeezing is one of the most recent and simple cell membrane disruption-mediated drug encapsulation approaches. In this method, cell membranes are perforated in a microfluidic setup due to rapid cell deformation during squeezing through constricted channels. While squeezing-based drug loading has been successful in loading drug molecules into various cell types, such as immune cells, cancer cells, and other primary cells, there is so far no comprehensive understanding of the pore opening mechanism on the cell membrane and the systematic analysis on how different channel geometries and squeezing speed influence drug loading. This article aims to develop a three-dimensional computational model to study the intracellular delivery for compound cells squeezing through microfluidic channels. The Lattice Boltzmann method, as the flow solver, integrated with a spring-connected network via frictional coupling, is employed to capture compound capsule dynamics over fast squeezing. The pore size is proportional to the local areal strain of triangular patches on the compound cell through mathematical correlations derived from molecular dynamics and coarse-grained molecular dynamics simulations. We quantify the drug concentration inside the cell cytoplasm by introducing a new mathematical model for passive diffusion after squeezing. Compared to the existing models, the proposed model does not have any empirical parameters that depend on operating conditions and device geometry. Since the compound cell model is new, it is validated by simulating a nucleated cell under a simple shear flow at different capillary numbers and comparing the results with other numerical models reported in literature. The cell deformation during squeezing is also compared with the pattern found from our compound cell squeezing experiment. Afterward, compound cell squeezing is modeled for different cell squeezing velocities, constriction lengths, and constriction widths. We reported the instantaneous cell center velocity, variations of axial and vertical cell dimensions, cell porosity, and normalized drug concentration to shed light on the underlying physics in fast squeezing-based drug delivery. Consistent with experimental findings in the literature, the numerical results confirm that constriction width reduction, constriction length enlargement, and average cell velocity promote intracellular drug delivery. The results show that the existence of the nucleus increases cell porosity and loaded drug concentration after squeezing. Given geometrical parameters and cell average velocity, the maximum porosity is achieved at three different locations: constriction entrance, constriction middle part, and outside the constriction. Our numerical results provide reasonable justifications for experimental findings on the influences of constriction geometry and cell velocity on the performance of cell-squeezing delivery. We expect this model can help design and optimize squeezing-based cargo delivery. 相似文献
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76.
Zhou Chao 《中外文化交流(英文版)》2005,(12):30-33
Huang Huilin, a South Korea student studying Chinese in Central China Normal University, graduated this year and was employed by a hotel in Qingdao, She said shyly to the reporter that she hoped to stay longer in China, "Although I have spent quite a long time in China, there are still much more alluring and mysterious to me," In recent years, there are more and more foreign students who have shared similar experiences with Huang. 相似文献
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78.
[目的/意义] 数字阅读是当前国民阅读的主要途径,付费数字阅读也成为新的消费增长点,但用户满意度仍然不高。文章尝试从用户需求层面进行探究,以期为数字阅读的持续健康发展提供指导,并对数字图书馆建设给予启发。[方法/过程] 通过收集大量相关产品的网络评论和与用户的深度访谈得到研究数据,进而运用扎根理论探究数字阅读产品的付费影响因素并构建出相对应的用户需求模型。[结果/结论] 研究发现,付费数字阅读用户需求分为功能需求和信息需求两个类别,且用户需求层次结构具有不断成长的演化趋势,这种趋势使得用户需求不断膨胀,从基本型需求发展到期望型需求,再拓展到兴奋型需求。 相似文献
79.
高校学生社团发展中的问题及对策——以仲恺农业工程学院为例 总被引:1,自引:0,他引:1
文章阐述了高校学生社团在发展中存在的主要问题及原因,尝试探寻高校学生社团发展的基本规律和有效途径. 相似文献
80.
全民健身计划的推出,为深化高校体育改革提供了依据。本文分析了高校体育存在的问题,提出了改革的对策,论述了如何提高大学生体育素质与促进全民健身的运动的开展。 相似文献