拉萨市少年宫的定位与管理

少年宫是青少年社会教育的主阵地,是学校教育的延伸和补充。在新时期,如何开发青少年的智力、促进素质教育的全面发展、提供学习表现的机会、丰富青少年课余校外生活,这就需要对其进行更准确的定位,探索建立更适合拉萨市少年宫管理的发展模式。     

既有斜拉桥桥面铺装更换施工分析

为保证重庆马桑溪长江大桥整治工程施工过程中的桥梁结构安全,对该桥整治施工全过程开展施工监控,对不同施工工况下的实桥进行了试验量测和仿真分析。有限元分析和实测结果表明：桥梁结构主梁位移和应力与斜拉索索力的实测结果与变化趋势均符合理论变化规律,且均控制在安全指标之内。桥面施工整治中的配重方案对主梁影响不明显,此后同类整治工程中可简化配重方案,即可一次施加配重或者验算通过后不施加配重。此外,同类工程可考虑将主梁线形作为主要监控指标,索力及应力作为辅助监控指标以指导施工。施工监控结果表明该整治工程的施工方案是切实可行的,可为今后国内外大跨径斜拉桥整治工程提供参考。     

中国瓷：见证历史友谊——从托普卡帕馆藏中国瓷器看中土文化交流

土耳其伊斯坦布尔的托普卡帕故宫博物馆历史上是奥斯曼土耳其帝国皇宫,现为土耳其国立博物馆,是世界珍藏中国古代瓷器最多者之一。其珍藏并展示的各类奥斯曼王朝珍品包括中国宋、元、明、清的各式瓷器10358件,堪称数量众多,门类齐全,对研究中国瓷器及了解中土两国文化交流的历史颇具参鉴意义。其馆藏中国瓷器主要为青瓷、青花瓷、单彩瓷和多彩瓷等,来源为贡品、礼品、战利品和外贸商品等。     

Study on the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if h TRF1 mutation is one of the factors of the activation oftelomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing.Real-time RT-PCR was used to detect the expression ofhTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of h TRF1 in 10 cell line cells. Results: h TRF1 gene, mapped to 8q 13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108～0.0749) than in normal mononuclear cells (0.0493, 0.0369～0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1,2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.     

系统集成项目工程质量管理有关问题探讨

系统集成项目的工程质量受人员、材料、机械、方法和环境等因素的影响。确保系统集成项目的工程质量要从做好勘察设计阶段质量管理,明确工程质量事故处理的基本要求和处罚标准等多方面着手。     

成才路上三块基石——访儿童教育专家孙云晓

去年。一场以现代亲子关系全面技巧与成功教育为主题的论坛在郑州国际会展中心隆重举行,儿童教育专家、中国青少年研究中心副主任、研究员．当年以一篇《夏令营中的较量》震撼全国的孙云晓先生与大家一起探讨了孩子的教育与成长。孙先生从教育的核心是培养孩子健康的人格、好的关系胜过许多教育、习惯决定孩子的命运三个方面讲解了现代青少年儿童的教育问题,近千名学生家长和学生参加了活动。记者对孙先生就青少年如何成才进行了专访。