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481.
Rajesh Kumar Kanoi 《海外英语》2003,(4):32-33
Ru Yun and San Hua were best friends. They had been the best of friends for almost half their lives, at about fifteen years of age. They went to the same school, took the same bus and shared the same interests. They were always together like a pair of Siamese twins. Nothing could separate them, not even their studies or their parents, 相似文献
482.
Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats 总被引:1,自引:0,他引:1
G. Kumar G. Sharmila Banu M. Rajasekara Pandian 《Indian journal of clinical biochemistry : IJCB》2007,22(1):105-108
The serum and hepatic enzymes of rats were studied after exposed to country made liquor (CML) along with two chelating agents
(glutathione and Selenium). There was a significant increase in several serum enzyme levels (viz., aspartate transaminase,
alanine transaminase, alkaline phosphatase, sorbitol dehydrogenase, glutamate dehydrogenase, bilirubin) and decrease in various
hepatic enzymes (Succinic dehydrogenase, Glucose 6-phosphatase, 5'Nucleotiease, Acid phosphatase, Acid ribonuclease, Cytochrome
P-450) due to repeated administration of CML (2ml/100g of body weight). Results of this study revealed that the GSH and Se
could give a significant protective action in serum and hepatic enzymes of CML exposed rats. 相似文献
483.
S. V. Suresh Babu M. M. Shareef A. Pavan Kumar Shetty K. Taranath Shetty 《Indian journal of clinical biochemistry : IJCB》2002,17(2):7-26
Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has
an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino
acids by modified method based on dinitrophenyl and HPLC profile involving pre-column derivatization with o-pthalaldehyde
(OPA) derivatization, respectively. The method, based on the alkylation of-SH groups prior to OPA derivatization of amino
acids followed by reverse phase high performance liquid chromatography, provide a comprehensive profile of more than twenty
amino acids (including-SH group containing) in a single run lasting about 45 minutes. The present study, apart from establishing
the normal profile of amino acids in plasma of Indian sub population, also presents HPLC profile for some of the rare amino
acidopathies. 相似文献
484.
Praveen Kumar Shetty T. N. Pattabiraman 《Indian journal of clinical biochemistry : IJCB》2004,19(1):97-101
The present study deals with the estimation of total carbohydrate, protein bound carbohydrate, bound fucose and sialic acid
along with total protein in disease conditions like gingivitis, periodontitis and their comparison with the normals.
The neutral hexose values in gingivitis (8.08±2.20mg/100mg protein) and periodontitis (12.5±2.16mg/ 100mg protein) decreased
significantly when expressed per 100 mg protein compared to normals (19.8±1.89mg/100mg protein). This might be because of
higher protein concentration in these two clinical conditions. The ethanol insoluble hexose values were significantly reduced
in both these conditions compared to controls (3.71±1.64,5.91±1.63,7.65±0.86mg/100mg protein respectively). The ethanol soluble
hexose values were also found to be drastically reduced. This decrease in saliva appears to be characteristic feature of oral
diseases. In gingivitis and periodontitis fucose level was found to be increased compared to normals when expressed as a function
of salivary volume. However in terms of protein concentration the values in gingivitis (2.95±1.59), periodontitis (3.26±0.98)
and normals (3.20±0.50mg/100mg) were not different. Sialic acid in ethanol insoluble fraction of salivary samples mg/100mg
protein was found to be significantly reduced in both gingivitis (0.78±0.33) and periodontitis (0.95±0.31) compared to controls
(1.92±0.33) 相似文献
485.
K. Rohini M. Surekha Bhat P. S. Srikumar A. Mahesh Kumar 《Indian journal of clinical biochemistry : IJCB》2016,31(3):332-335
The aim of the study was the assessment of hematological parameters in pulmonary tuberculosis patients. Forty patients diagnosed with tuberculosis were recruited from the Institute of Thoracic Medicine on the basis of history, clinical examination, chest radiography, sputum examination and related laboratory parameters and were compared with age and sex matched healthy volunteers (n = 40). Hematological parameters and CRP in tuberculosis patients were determined. The mean values for serum hemoglobin level, RBC count and platelet count in PTB was found to be less (p < 0.001). Erythrocyte sedimentation rate (ESR), plasma C-reactive protein, WBC count in PTB subjects was increased (p < 0.001 for ESR & CRP, p < 0.05 for WBCs) and all were statistically significant. This study demonstrated that serum hemoglobin level, RBC count and platelet count was decreased in tuberculosis patients whereas ESR, CRP and WBC count was increased when compared with healthy controls. 相似文献
486.
This study was carried out to estimate the levels of glutathione peroxidase and selenium in blood of abortion cases. Glutathione
peroxidase and selenium were determined in 52 abortion cases (22 in 1st trimester, 30 in second trimester), 45 normal pregnant cases and 25 nonpregnant control cases. The selenium concentration
in whole blood and plasma in abortion cases was almost the same as in normal pregnant women but significantly low when compared
with the control non-pregnant group. The glutathione levels was higher in abortion cases when compared with normal pregnant
and non-pregnant control groups. Red cell and plasma glutathione peroxidase activities of women who had abortion were significantly
lower compared with both non-pregnant control group and normal pregnancies. 相似文献
487.
A. S. Bhatia Satish Kumar B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(2):1-5
Tuberculosis is still a major health problem in most developing countries and its incidence is rising in many developed countries.
This resurgence has been attributed to the HIV epidemic and TB has been declared as a global health emergency by WHO in 1993.
The diagnosis of tuberculosis mainly depends upon initial clinical suspicion and radiographic findings with subsequent bacteriological
confirmation by sputum smear examination and culture. Lack of sensitivity in smear examination, non specificity of radiological
findings, extended tum around time ofMycobacterium tuberculosis culture and difficulties in diagnosing paucibacillary, childhood and extrapulmonary tuberculosis has necessitated to explore
the utility of immunodiagnosis of tuberculosis as a convenient and cost effective test to supplement clinical information
for definite diagnosis. Many commercial tests are available in the market for diagnosis of TB. Most of these tests are based
on the detection of IgG, IgA and IgM antibodies to specific mycobacterial antigen or mixture of antigens. Indigenous immunoassay
systems have explored excretory-secretory ES-31 mycobacterial antigen for immunodiagnosis of TB. Many a time there is lack
of consistent elevation in all the three Ig classes in active infection thus making it more important to determine the ideal
antibody isotype assay for reliable diagnosis of tuberculosis and to save the costs of the patient for unnecessary investigations. 相似文献
488.
In an increasingly globalizing and knowledge-based world economy, the declining importance of R&D activity in India is a matter of concern. This paper analyzes the determinants of R&D behaviour of Indian enterprises over the 1990s in the context of the reforms of 1991 and their impact on the R&D behaviour of MNE affiliates and local enterprises. The analysis suggests that although average levels of spending have fallen, increased competition due to liberalization seems to have pushed local firms to rationalize their R&D activity and make it more efficient. Also, R&D spending seems to rise more than proportionally with firm size after a certain threshold level has been reached. The analysis brings out differences in the nature of R&D activity of MNE affiliates and local firms. Local firms direct their R&D activity primarily towards the assimilation of imported technology, and to providing a backup to their outward expansion via exports and FDI. MNE affiliates, on the other hand, focus on exploiting the advantages of India as an R&D platform for their parents. The paper is concluded with some policy implications of the findings. 相似文献
489.
D. H. Shah Ajay Kumar M. G. R. Rajan U. R. Thakare S. M. Sharma 《Indian journal of clinical biochemistry : IJCB》1989,4(1):14-22
An external quality assessment was conducted for RIA of thyroid related hormones. Thirtyfive laboratories (35 for T4, 34 for T3 and 23 for TSH) from different parts of country participated in the programme. Twentyfour samples (16 pools: 5 simple and 11 manipulated pools) in 8 batches, 3 per batch per month were sent for analysis of T4, T3 and TSH. Some of the samples were repeated 3 times at different occasions to assess the imprecision of the laboratory. The overall mean percent CV obtained for T4, T3 and TSH were 22.7, 36.32 and 52.38 respectively. The recovery for added T4 was 86.73% while that for T3 was 117.4%. A large variation was obtained for recovery of TSH. For T4 estimations, 13 laboratories had a desirable performance i. e. bias less than ±10.0% and variability of bias (VB) and imprecision (IP) less than 15.0%. None of the laboratories had a desirable performance for T3 or TSH. The number of laboratories with acceptable performance i. e. bias between ±10.0–15.0%, VB and IP between 20.0–25.0% for T4, T3 and TSH were 4, 3 and 0 respectively. The number of laboratories which required attention (bias between ±15.0–20.0%; VB and IP between 20.0–25.0%) were 5,7 and 1 respectively. The unacceptable results with larger bias, VB and IP for T4, T3 and TSH were 6, 18 and 17 respectively. Our results are in general agreement that the performance of T4 assay is better than T3 and both in turn are much better than TSH. Quantitation of circulating thyroid hormones (TH) viz. tri-iodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH), which form the largest percentage of hormones estimated in a clinical laboratory is mainly done by radio-immunoassay (RIA) procedures. The reliability and reproducibility of these assays are generally monitored by using internal quality control (IQC) samples in every assay batch. Thus, the IQC provides information whether the assay results are satisfactory and can be released. However, external quality assessment (EQA) is a procedure whereby an external agency undertakes evaluation of the quality of an analytical service by providing samples for analysis to individual laboratories performing the assay. The data gathered is analysed collectively. EQA therefore provides a means by which performance of a laboratory is assessed in relation to other laboratories and matching the assay unbiased by removing systematic error, if present. This is important since RIA procedure involves several steps (collection and storage of samples, quality of the reagents, procedure followed for performance of an assay, counting equipment used and the mode of the data analysis) and therefore prone to systematic errors. We therefore undertook the EQA programme for assessment of thyroid related hormones as a joint collaborative project of Bhabha Atomic Research Centre, India and International Atomic Energy Agency. 相似文献
490.
Satya Vati Rana Raj Kumar Singhal Kartar Singh Lata Kumar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):5-9
Adenosine deaminase activity (ADA) was estimated in cerebrospinal fluid (CSF) of 30 patients of tuberculous meningitis (TBM)
and 10 patients each of partially treated pyomeningitis (PTM), aseptic meningitis (AM) and pyogenic meningitis (PM). Mean
ADA levels in CSF of TBM patients were higher (18.22 U/L) as compared to 6.28 U/L, 3.43 U/L and 7.98 U/L in PTM, AM and PM
respectively. This difference of ADA values in CSF between TBM and other types of meningitis was statistically significant
(p<0.01) different. Sensitivity and specificity of ADA levels in CSF of children to diagnose tuberculous meningitis was 66.6%
and 90% respectively at 10 U/L cut off of ADA levels in CSF. ADA levels in CSF could also differentiate PTM, AM and PM from
TBM with a specificity of 90%, 100% and 80% respectively. 相似文献