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Patra SK Jain A Sherwal BL Khanna A 《Indian journal of clinical biochemistry : IJCB》2010,25(3):315-318
To detect the site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-TB by DNA sequencing. 50 MDR-TB patients
were enrolled in our study after informed written consent. Mycobacterial DNA was extracted from sputum samples by Universal
Sample Processing (USP) method and RRDR of rpo B gene was amplified by PCR using primers RP4T and RP8T and then sequenced
by automated DNA sequencing. The nucleotide sequences of RRDR of rpo B gene were compared with the reference sequence. We
observed three different types of mutation in the RRDR of rpo B gene. The frequency of mutation in codon 531 (TCG → TTG),
526 (CAC → TAC) and 516 (GAC → GTC) are 60, 26.6 and 6.6% respectively. Of the total cases studied, 6.6% cases, although resistant
to rifampicin, did not show any mutation in the RRDR of rpo B gene. Codon 531 (TCG → TTG) is the most common site of mutation
in RRDR of rpo B gene for rifampicin resistance in MDR-pulmonary tuberculosis followed by codon 526 (CAC → TAC) and codon
516 (GAC → GTC). 相似文献
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The popularity of forensic crime shows such as CSI has fueled debate about their potential social impact. This study considers CSI's potential effects on public understandings regarding DNA testing in the context of judicial processes, the policy debates surrounding crime laboratory procedures, and the forensic science profession, as well as an effect not discussed in previous accounts: namely, the show's potential impact on public understandings of DNA and genetics more generally. To develop a theoretical foundation for research on the "CSI effect," it draws on cultivation theory, social cognitive theory, and audience reception studies. It then uses content analysis and textual analysis to illuminate how the show depicts DNA testing. The results demonstrate that CSI tends to depict DNA testing as routine, swift, useful, and reliable and that it echoes broader discourses about genetics. At times, however, the show suggests more complex ways of thinking about DNA testing and genetics. 相似文献
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Behrmann BL 《The Journal of perinatal education》2003,12(4):vi-vx
In the second of a two-part article, the author encourages women to learn about their mothers' and grandmothers' birth experiences as a way to celebrate and record their family's legacy of childbirth. 相似文献