Adiponectin Gene Polymorphism and its Association with Type 2 Diabetes Mellitus

Mutations in different regions of adiponectin gene have been reported to be associated with obesity, atherosclerosis and type 2 diabetes mellitus. The present study was aimed to investigate the association among SNP 45 T > G of adiponectin gene and type 2 diabetes in South Indian population. 75 clinically diagnosed case of type 2 diabetes were studied and compared with 75 apparently healthy controls. The genotype frequency of SNP45 T > G in exon 2 of adiponectin gene was determined by PCR based restriction enzyme analysis using the restriction enzyme SmaI. (recognition site: CCC↓GGG). Three kind of genotypes: wild type TT (470 bp), heterozygous type TG (470 bp, 336 bp, 134 bp) and homozygote mutant type GG (336 bp, 134 bp) were studied. A positive association has been found between SNP45 T > G and type 2 diabetes in the study population (P = 0.010, OR = 3.797, 95% CI = 1.312–10.983). Therefore, SNP45T > G in adiponectin gene may be one of the risk factors for type 2 diabetes.     

Biomarkers of inflammation and cardiac remodeling: the quest of relevant companions for the risk stratification of heart failure patients is still ongoing

Heart failure (HF) is a frequent chronic disease in elderly people and represents a major health and economic burden. The accurate diagnosis of HF and the identification of high risk patients is mandatory to select the appropriate therapy and care. HF is associated to cardiac remodeling and inflammation and to the release of neurohormones. If natriuretic peptides remain the biomarkers of choice for the diagnosis and the prognosis of HF, several biomarkers related to cardiac remodeling and inflammation are available for testing and may add valuable information for the risk estimation of HF patients and for the selection of the most appropriated therapy. Furthermore, the quest for new efficient biomarkers of cardiac remodeling and inflammation is still ongoing and new candidates have been identified for a potential use in daily clinical practices. However, before their potential translation to routine parameters several challenges ranging form the analytical validation to the cost-effectiveness estimation should be considered.     

Shrink-film microfluidic education modules: Complete devices within minutes

As advances in microfluidics continue to make contributions to diagnostics and life sciences, broader awareness of this expanding field becomes necessary. By leveraging low-cost microfabrication techniques that require no capital equipment or infrastructure, simple, accessible, and effective educational modules can be made available for a broad range of educational needs from middle school demonstrations to college laboratory classes. These modules demonstrate key microfluidic concepts such as diffusion and separation as well as "laboratory on-chip" applications including chemical reactions and biological assays. These modules are intended to provide an interdisciplinary hands-on experience, including chip design, fabrication of functional devices, and experiments at the microscale. Consequently, students will be able to conceptualize physics at small scales, gain experience in computer-aided design and microfabrication, and perform experiments-all in the context of addressing real-world challenges by making their own lab-on-chip devices.     

Disposable parallel poly(dimethylsiloxane) microbioreactor with integrated readout grid for germination screening of Aspergillus ochraceus

In this work a disposable, parallel microbioreactor (MBR) suitable for screening in batch or continuous mode is presented. The reactor consists of five parallel microchambers made of poly(dimethylsiloxane) bonded to a glass substrate. A grid structure is engraved on each chamber, allowing subsequent morphology imaging. Measurements are recorded over the entire cultivation period with constant parameters, namely, position and focus in the z-axis. The microdevice may be used for either parallel, uni- or multiparametric screening, and overcomes the drawback of gridless microwell plates which require expensive equipment such as an inverted microscope with an automatic stage. To validate the scalability from laboratory scale to microscale, and thus the cultivation protocol in the MBR, the germination of fungal spores (A. ochraceus) is evaluated for two different key magnitudes (pH and temperature) and compared to the results obtained from conventional laboratory scale systems (flasks and agar plates). Information on germination capacity with regard to interspecies' variability allows for optimization of industrial processes as optimal pH and temperature matched to the mesoscopic cultivation systems. The germination conditions therefore remain unaffected inside the MBR, while providing the following advantages: (i) dramatic reduction of medium consumption, (ii) submerged cultivation with constant oxygen supply, (iii) assured low cost and disposability, and (iv) possibility of a continuous cultivation mode.     

Development and initial validation of the Psychological Characteristics of Developing Excellence Questionnaire

The development of talent is a complex process mediated by a host of psychological, social, physical, and environmental variables. Unfortunately, the multiple processes involved in talent development are frequently ignored by the systems and protocols employed in sport. Modern approaches to talent development are beginning to stress the initial possession, then subsequent development, of generic psychological characteristics (e.g. psychological characteristics of developing excellence; MacNamara, Button, & Collins, 2010a , 2010b ) as the best way to realize latent potential. Accordingly, this paper describes the development and initial validation of the Psychological Characteristics of Developing Excellence Questionnaire (PCDEQ). In the first phase, an initial list of 160 items was developed. A combination of expert panel reviews, cognitive interviews, and a pilot test was used to assess the relevance, representativeness, and validity of each item. Ninety-six items were retained following these steps. Exploratory factor analysis, with a sample of 363 athletes, revealed an interpretable 59-item, 6-factor solution with good internal consistency (0.870, 0.866, 0.847, 0.741, 0.749, and 0.701 respectively). The Psychological Characteristics of Developing Excellence Questionnaire would appear to hold promise as a useful tool to provide coaches and athletes with information about the psychological characteristics of developing excellence that are being properly addressed or neglected during different stages of development or in different contexts.     

Participation-related relative age effects in Masters swimming: a 6-year retrospective longitudinal analysis

Medic and colleagues (Medic, Starkes, & Young, 2007) found that relatively younger cohorts of Masters athletes had higher participation rates and achieved higher performances compared with a relatively older cohort. Considering that Medic and colleagues' (2007) study was cross-sectional in nature, the purpose of this investigation was to employ a retrospective longitudinal study design to examine the participation rates of Masters swimmers as a function of an individual's constituent year within any 5-year age category over a period of 6 years. Using archived data from the 2003 to 2009 US Masters Short Course National Championships, swimmers' attendance was followed for a period of six consecutive years. Results indicated that a participation-related relative age effect was observed among swimmers who, over a period of 6 years, competed in either at least one championship (N = 2596; Cochran's Q? = 64.16, r(s) = -0.92, both P < 0.0001) or at least three championships (N = 441; Cochran's Q? = 47.51, r(s) = -0.91, both P < 0.0001). Overall, effect size analyses indicated that the odds of a Masters swimmer participating in the championship during the first constituent year of any 5-year age category was more than two times greater than the odds of that athlete participating during the fifth constituent year.     

The effect of a supported exercise programme in patients with newly diagnosed Type 2 diabetes: a pilot study

The aim of this study was to examine the effectiveness of either a standard care programme (n = 9) or a 12-week supported exercise programme (n = 10) on glycaemic control, β-cell responsiveness, insulin resistance, and lipid profiles in newly diagnosed Type 2 diabetes patients. The standard care programme consisted of advice to exercise at moderate to high intensity for 30 min five times a week; the supported exercise programme consisted of three 60-min supported plus two unsupported exercise sessions per week. Between-group analyses demonstrated a difference for changes in low-density lipoprotein cholesterol only (standard care programme 0.01 mmol · L(-1), supported exercise programme -0.6 mmol · L(-1); P = 0.04). Following the standard care programme, within-group analyses demonstrated a significant reduction in waist circumference, whereas following the supported exercise programme there were reductions in glycosylated haemoglobin (6.4 vs. 6.0%; P = 0.007), waist circumference (101.4 vs. 97.2 cm; P = 0.021), body mass (91.7 vs. 87.9 kg; P = 0.007), body mass index (30.0 vs. 28.7 kg · m(-2); P = 0.006), total cholesterol (5.3 vs. 4.6 mmol · L(-1); P = 0.046), low-density lipoprotein cholesterol (3.2 vs. 2.6 mmol · L(-1); P = 0.028), fasting β-cell responsiveness (11.5 × 10(-9) vs. 7.0 × 10(-9) pmol · kg(-1) · min(-1); P = 0.009), and insulin resistance (3.0 vs. 2.1; P = 0.049). The supported exercise programme improved glycaemic control through enhanced β-cell function associated with decreased insulin resistance and improved lipid profile. This research highlights the need for research into unsupported and supported exercise programmes to establish more comprehensive lifestyle advice for Type 2 diabetes patients.     

Quadriceps muscle blood flow and oxygen availability during repetitive bouts of isometric exercise in simulated sailing

In this study, we wished to determine whether the observed reduction in quadriceps muscle oxygen availability, reported during repetitive bouts of isometric exercise in simulated sailing efforts (i.e. hiking), is because of restricted muscle blood flow. Six national-squad Laser sailors initially performed three successive 3-min hiking bouts followed by three successive 3-min cycling tests sustained at constant intensities reproducing the cardiac output recorded during each of the three hiking bouts. The blood flow index (BFI) was determined from assessment of the vastus lateralis using near-infrared spectroscopy in association with the light-absorbing tracer indocyanine green dye, while cardiac output was determined from impedance cardiography. At equivalent cardiac outputs (ranging from 10.3±0.5 to 14.8±0.86 L · min(-1)), the increase from baseline in vastus lateralis BFI across the three hiking bouts (from 1.1±0.2 to 3.1±0.6 nM · s(-1)) was lower (P = 0.036) than that seen during the three cycling bouts (from 1.1±0.2 to 7.2±1.4 nM · s(-1)) (Cohen's d: 3.80 nM · s(-1)), whereas the increase from baseline in deoxygenated haemoglobin (by ～17.0±2.9 μM) (an index of tissue oxygen extraction) was greater (P = 0.006) during hiking than cycling (by ～5.3±2.7 μM) (Cohen's d: 4.17 μM). The results suggest that reduced vastus lateralis muscle oxygen availability during hiking arises from restricted muscle blood flow in the isometrically acting quadriceps muscles.     

Creativity, expertise, and attention: exploring their development and their relationships

Recent evidence from neuroscience suggests that creativity is developed early in life and that the greatest improvements in creativity can be expected during this time. Therefore, the aim of this study was to investigate the creative development of children depending on their level of expertise and attention processes. Moreover, the significance of general and specific attention components and special training effects in the development of specific and non-specific creative thinking remain unclear. Thus, skilled (team handball) and non-skilled children (n = 120) aged 7, 10, and 13 completed two divergent thinking tasks (specific/non-specific) and two attention tasks (specific/non-specific) in a cross-sectional design. It is evident that general and sport-specific creativity have similar paths of development. Skilled players with high attention scores performed better than skilled players with low attention scores, in accordance with specific creative thinking abilities. In contrast, and in accordance with general creative thinking abilities, non-skilled players with less attentional skills outperformed non-skilled players.     

Effects of continuing professional development on urban elementary students' knowledge

This study supports the important role teacher training programs can play in increasing physical education knowledge. In this project, teachers were able to increase students' understanding of critical physical activity/fitness knowledge through physical education instruction, without changing the physical education contact time. This link to student needs as well as the school's overall mission is a promising one for students and schools alike.