Environmental learning in regions: a social capital based approach. The case of Latvia

How do people learn about the environment and behave accordingly? What is the cognitive process at the base of this learning mechanism? The present paper is a pilot work investigating the dynamics of individual environmental knowledge on the basis of social capital theory. Using Tsai and Ghoshal’s findings, a well known framework widely accepted in organizational studies which supports the existence of a relationship between structural, relational and cognitive dimensions of social capital, and between social capital and knowledge exchange, these dynamics are then tested in the context of environmental behaviour studies. The quantitative analysis is carried out by means of a structural equation model based on data collected in the Republic of Latvia. Results support main theoretical hypotheses, but several effects are found to be highly sensitive to control variables (residence place dimensions, ethnic affiliation, age, personal income), leading to hypothesise a strong dependency of environmental learning patterns on the socio-demographic, socio-economic and cognitive context.     

Rapid inertial solution exchange for enrichment and flow cytometric detection of microvesicles

Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered by difficulty in isolation and quantification due to their size and the complexity of biological samples. Conventional approaches to improved isolation require specialized equipment and extensive sample preparation time. Therefore, isolation and detection methods of exosomes will benefit biological and clinical studies. Here, we report a microfluidic platform for inline exosome isolation and fluorescent detection using inertial manipulation of antibody-coated exosome capture beads from biological fluids.     

Live capture and reuse of project knowledge in construction organisations

Knowledge Management Research & Practice - It is important that the knowledge generated on construction projects is captured and shared between project team members for continuous improvement,...     

Educational research at international level: Features, expectations and limitations

This paper offers a short review of the major characteristics and limitations of international educational research institutions. One of the major concerns of the paper is to constrast the role of international vis à vis national institutions in educational research. The complementarity of international and national institutions is manifested in the selection of topics, application of approaches, use of methods and organisation of work usually applied in international research programmes, but rarely used in national projects. The article concludes by drawing attention to the fact that the variety of international research institutions alone cannot satisfy evolving needs in the educational field, but should be accompanied by sufficient flexibility for continuous adaptation.     

High-throughput size-based rare cell enrichment using microscale vortices

Cell isolation in designated regions or from heterogeneous samples is often required for many microfluidic cell-based assays. However, current techniques have either limited throughput or are incapable of viable off-chip collection. We present an innovative approach, allowing high-throughput and label-free cell isolation and enrichment from heterogeneous solution using cell size as a biomarker. The approach utilizes the irreversible migration of particles into microscale vortices, developed in parallel expansion-contraction trapping reservoirs, as the cell isolation mechanism. We empirically determined the critical particle∕cell diameter D(crt) and the operational flow rate above which trapping of cells∕particles in microvortices is initiated. Using this approach we successfully separated larger cancer cells spiked in blood from the smaller blood cells with processing rates as high as 7.5×10(6) cells∕s. Viable long-term culture was established using cells collected off-chip, suggesting that the proposed technique would be useful for clinical and research applications in which in vitro culture is often desired. The presented technology improves on current technology by enriching cells based on size without clogging mechanical filters, employing only a simple single-layered microfluidic device and processing cell solutions at the ml∕min scale.