Fabrication of a gel particle array in a microfluidic device for bioassays of protein and glucose in human urine samples

This paper describes a simple method for fabricating a series of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures inside microfluidic channels as probe for proteins and glucose. In order to demonstrate the feasibility of this newly developed system, bovine serum albumin (BSA) was chosen as a model protein. PEG microcolumns were used for the parallel detection of multiple components. Using tetrabromophenol blue (TBPB) and the horseradish peroxidase/glucose oxidase reaction system, bovine serum albumin (BSA) and glucose in human urine were detected by color changes. The color changes for BSA within a concentration range of 1-150 μM, and glucose within a range of 50 mM-2 M could be directly distinguished by eyes or precisely identified by optical microscope. To show the practicability of the gel particle array, protein and glucose concentrations of real human urine samples were determined, resulting in a good correlation with hospital analysis. Notably, only a 5 μL sample was needed for a parallel measurement of both analytes. Conveniently, no special readout equipment or power source was required during the diagnosis process, which is promising for an application in rapid point-of-care diagnosis.     

The rating of perceived exertion predicts intermittent vertical jump demand and performance

The aims of this study were (a) to assess the ability of the rating of perceived exertion (RPE) to predict performance (i.e. number of vertical jumps performed to a fixed jump height) of an intermittent vertical jump exercise, and (b) to determine the ability of RPE to describe the physiological demand of such exercise. Eight healthy men performed intermittent vertical jumps with rest periods of 4, 5, and 6 s until fatigue. Heart rate and RPE were recorded every five jumps throughout the sessions. The number of vertical jumps performed was also recorded. Random coefficient growth curve analysis identified relationships between the number of vertical jumps and both RPE and heart rate for which there were similar slopes. In addition, there were no differences between individual slopes and the mean slope for either RPE or heart rate. Moreover, RPE and number of jumps were highly correlated throughout all sessions (r = 0.97-0.99; P < 0.001), as were RPE and heart rate (r = 0.93-0.97; P < 0.001). The findings suggest that RPE can both predict the performance of intermittent vertical jump exercise and describe the physiological demands of such exercise.     

绿色的田野

~~绿色的田野@T.吉尔基森!美国 @R.德尔!美国 @F.米勒 @陶桂年~~     

地理信息系统

1、地理信息系统(GIS)的基本概念 迈克尔,布莱克莫尔对制图自动化和地理信息系统的评论在(《Hluman Geograp-hy》)杂志上巳出现几年了,但现是首次对GIS进行较为高水平的全面评论。文章开头,对此领域进行一些说明是很适当的,尤其澄清目前此领域在性质和意义上的广泛混淆。同一个月里,美国地理学会主席可能会看到“区域地理、文化地理和历史地理……蜂拥前往地理     

鱼与熊掌如何兼得？

在过去20多年中，美国高校的本质发生了剧烈的变化。由于越来越多的人把高等教育视为形成自身优势的途径，所以各高校便借机不断提高教育收费．其结果是许多公立大专院校不再依赖州政府的财政拨款，其运作模式更像私立院校。与此同时，许多私立院校——特别是那些获得社会捐助很少、比以往更加依赖学费和研究补助收入的院校，事实上已经完全放弃了其对社会、州和国家应尽的文化与经济义务。     

The Intertidal Pulmonate, Onchidium Tumidium,Uses Different Strategies to Survive Short Term and Long Term Anoxia

Onchidiumtumidiumisaslug-likepulmonatecommonlyfoundinthebrackishmangroveswampsinSingapore.Itleadsanamphibiouslifeonthemudflatsintheintertidalzonewhereitisexposedonlyduringebbtideswhichlastsnomorethan12h.Druingthisperiod,itemergesfromitsburrow,andgrazesonthesurfacealgae.Asthetiderises,itburrowsanddisappearsintothemud.Hence,itisnaturallyexposedto6～12hofenvironmentalhypoxiaeveryday.Thisperiodofhypoxiacanalsobeextendedto24～48hduringcoolorrainydays,wherethepulmonateabstainsfromfeeding.SinceO.tu…