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41.
Specific binding and magnetic concentration of CD8+ T-lymphocytes on electrowetting-on-dielectric platform 总被引:1,自引:0,他引:1
In the quest to create a low-power portable lab-on-a-chip system, we demonstrate the specific binding and concentration of human CD8+ T-lymphocytes on an electrowetting-on-dielectric (EWOD)-based digital microfluidic platform using antibody-conjugated magnetic beads (MB-Abs). By using a small quantity of nonionic surfactant, we enable the human cell-based assays with selective magnetic binding on the EWOD device in an air environment. High binding efficiency (~92%)of specific cells on MB-Abs is achieved due to the intimate contact between the cells and the magnetic beads (MBs) produced by the circulating flow within the small droplet. MBs have been used and cells manipulated in the droplets actuated by EWOD before; reported here is a cell assay of a clinical protocol on the EWOD device in air environment. The present technique can be further extended to capture other types of cells by suitable surface modification on the MBs. 相似文献
42.
Naturalistic Language Recordings Reveal “Hypervocal” Infants at High Familial Risk for Autism 下载免费PDF全文
Meghan R. Swanson Mark D. Shen Jason J. Wolff Brian Boyd Mark Clements James Rehg Jed T. Elison Sarah Paterson Julia Parish‐Morris J. Chad Chappell Heather C. Hazlett Robert W. Emerson Kelly Botteron Juhi Pandey Robert T. Schultz Stephen R. Dager Lonnie Zwaigenbaum Annette M. Estes Joseph Piven the IBIS Network 《Child development》2018,89(2):e60-e73
Children's early language environments are related to later development. Little is known about this association in siblings of children with autism spectrum disorder (ASD), who often experience language delays or have ASD. Fifty‐nine 9‐month‐old infants at high or low familial risk for ASD contributed full‐day in‐home language recordings. High‐risk infants produced more vocalizations than low‐risk peers; conversational turns and adult words did not differ by group. Vocalization differences were driven by a subgroup of “hypervocal” infants. Despite more vocalizations overall, these infants engaged in less social babbling during a standardized clinic assessment, and they experienced fewer conversational turns relative to their rate of vocalizations. Two ways in which these individual and environmental differences may relate to subsequent development are discussed. 相似文献
43.
V. K. Verma V. Ramesh Satyendra Tewari R. K. Gupta Nakul Sinha C. M. Pandey 《Indian journal of clinical biochemistry : IJCB》2005,20(2):68-74
A study has been carried out on 250 CAD patients to see how the serum levels of three antioxidants i.e., Vitamin C, Bilirubin
and Ceruloplasmin are related to the CAD risk factors and characteristics in these patients. The number of severe category
CAD patients declined by 7–18% with increasing serum levels of the three antioxidants and, triple vessel disease declined
by 14–20%. A decline of 39% in Myocardial Infarction (MI) occurred with increasing serum Ceruloplasmin. Serum Ceruloplasmin
was significantly lower in the MI group compared to the non MI group CAD patients. There was a steady and a significant decline
in the mean values of serum levels of cholesterol, Triglyceride, VLDL cholesterol along with BMI with increasing serum level.
The study indicates that with increasing serum levels of the three antioxidants studied, the % MI along with % Triple vessel
disease and severity of CAD goes down suggesting that the modifiable risk factors have to be suitably modified in order to
maintain a reasonably high level of these antioxidants, as the risk factors are inversely related to the serum antioxidant
levels. 相似文献
44.
Rama Devi Mittal Amita Pandey Balraj Mittal Kailash Nath Agarwal 《Indian journal of clinical biochemistry : IJCB》2002,17(2):1-6
Eight weeks of latent iron deficiency in weaned female rats of Sprague Dawley strain maintained on experimental low-iron diet
(18–20 mg/Kg) did not significantly change the gross body weight and tissue weights of brain and liver. Packed cell volume
(PCV) and hemoglobin concentration remained unaltered. However, non-heme iron content in liver and brain decreased significantly
(P<0.001). The activities of glutamate dehydrogenase, glutamic acid decarboxylase, and GABA-transaminase (GABA-T) in brain
decreased by 15%, 11.4% and 25.7% respectively. However, this decrease was not statistically significant. Binding of3H Muscimol at pH 7.5 and 1 mg protein/assay increased by 143% (P<0.001) in synaptic vesicular membranes from iron-deficient
rats as compared to the controls.3H glutamate binding to the synaptic vesicles was also carried out under similar condition. However, the L-glutamate binding
was reduced by 63% in the vesicular membranes of iron deficient animals. These studies in dicate that iron plays an important
functional role in both excitatory and inhibitory neurotransmitter receptors. 相似文献
45.
Sangeeta Agnihotri S. B. Pachori D. N. Pandey M. C. Pant 《Indian journal of clinical biochemistry : IJCB》1990,5(2):91-94
The effects of regular feeding ofPyrus malus whole fruit and its juice on total serum cholesterol, HDL cholesterol, LDL cholesterol, triglycerides and phospholipids have been studied in normal albino rabbits. It was observed that the whole fruit as well as juice caused significant and progressive decrease in total serum cholesterol, LDL cholesterol and triglycerides within a period of 30 days. On the other hand, serum phospholipids and HDL cholesterol showed progressive and significant increase. Apparently, whole fruit appeared to bring about more per cent lowering in total cholesterol, LDL cholesterol and more increase in HDL cholesterol as compared to juice. Whole apple fruit also caused increase in body weight of rabbits on 30th day (p<0.05) while oral administration of juice showed insignificant change in body weight even on 30th day (p>0.05). 相似文献
46.
K. K. Mishra H. P. Pandey R. H. Singh 《Indian journal of clinical biochemistry : IJCB》2007,22(2):41-43
Present clinical study involved two groups of psychosomatic disorders, bronchial asthma and rheumatoid arthritis. In the study,
the levels of plasma cortisol, blood glucose, total cholesterol and triglycerides were estimated in 125 clinical subjects,
(50 normal controls, and 40 having bronchial asthma and 35 suffering from rheumatoid arthritis. The results showed a significant
change in the levels of plasma cortisol and blood glucose in both the stressed clinical groups' vis-à-vis normal controls.
The levels of atherogenic lipids (total cholesterol and triglycerides) were found quite elevated in both the diseased groups.
However, in rheumatoid arthritis, the physiological changes were relatively more pronounced. The findings of this study indicate
that rheumatoid arthritis is a relatively more chronic and late onset disorder as the functional performance of hypothalamopituitary-adrenocortical
axis gradually declines with passage of time and the ability of the adrenocortical response to return to normalcy becomes
impaired. 相似文献
47.
Ajai Kumar M. C. Pant H. N. Pandey J. L. Aggarwal R. S. Sharma L. D. Joshi S. Singh 《Indian journal of clinical biochemistry : IJCB》1993,8(1):47-50
Effect of feeding ripe and unripe papaya fruit pulps were investigated on blood lipid profile of normal male adult albino rabbits by giving them pulps separately at the dose of 15 and 30g per day along with standard diet over a period of six weeks. As compared to controls both fruit pulps resulted in highly significant (p<0.001) and consistent lowering of serum cholesterol, low density lipoprotein cholesterol and triglycerides and elevation in phospholipids and high density lipoprotein cholesterol levels at 1,3 and 6 weeks of fruit pulp feeding indicating a dose dependent effect. Observations clearly support medicinal value ofC. Papaya in the management of atherosclerosis, fatty liver, ischaemic changes and consequential myocardial infarction. 相似文献
48.
Despite considerable progress made, a significant proportion of children continue to drop out before reaching secondary school in India. This study investigates factors influencing parental decision-making with regard to children's secondary schooling in the context of a rural area of Uttar Pradesh. The study finds that cost, distance to the nearest secondary school, membership in traditionally marginalised social groups, and being a girl child have a significant effect on a child's chance of transition. It also finds that factors influencing participation at the primary level may influence the transition decision, having key implications for education policy formulation across education levels. 相似文献
49.
Arun Kumar Singh Sunita Tiwari Abhishek Gupta Kamla Kant Shukla Kumar Gaurav Chhabra Achileshwar Pandey Aditya Bhushan Pant 《Indian journal of clinical biochemistry : IJCB》2015,30(3):255-262
Metabolic syndrome (MetS) is a cluster of interrelated common clinical disorders. The role of resistin in insulin sensitivity and MetS is controversial till date. So, the aim of the present study was to investigate the relationship of plasma resistin levels with markers of the MetS in Indian subjects. In a case control study, total 528 subjects were selected for the study. 265 (194 male and 71 female) were cases (with MetS) and 263 (164 male and 99 female) were controls (without MetS). Required anthropometric measurements and calculations were carried out accordingly. All the Biochemical estimations were carried out according to standard protocol. Resistin level was measured by the standard protocol (By ELISA i.e. enzyme linked immunosorbent assay) as illustrated in the kit. Insulin level was also measured by the standard protocol as illustrated in the kit and insulin resistance was calculated by the standard procedures. Plasma resistin levels were significantly higher in cases compared with controls (male = 13.05 ± 4.31 vs. 7.04 ± 2.09 ng/ml; p ≤ 0.001 and female = 13.53 ± 4.14 vs. 7.42 ± 2.30 ng/ml; p ≤ 0.001). Plasma resistin levels were well correlated with waist circumference, glucose, triglycerides, waist/hip ratio, systolic and diastolic blood pressure, high density lipoprotein, total cholesterol, serum low density lipoprotein, serum very low density lipoprotein, insulin and insulin resistance. Plasma resistin levels were elevated in presence of the MetS and were associated with increased metabolic risk factors. 相似文献
50.
Vaibhav Pandey 《Resonance》2016,21(5):439-446
It is well known that the set C[0, 1] of real-valued continuous functions on the closed interval [0, 1] has a natural ring structure. Its maximal ideals are known to be points. Here we show that although there exist prime ideals which are not maximal, the zero set is again a singleton. In particular, each prime ideal is contained in a unique maximal ideal. 相似文献