冰球运动员的专项耐力

提高高级冰球运动员的运动水平有两个途径,一是提高运动员的身体机能;二是提高技、战术水平.如果提高身体机能,就必须准确地掌握运动员现有的专项耐力水平和专项耐力(包括有氧和无氧耐力)的提高过程.在冰球运动员的综合测试中使用在塑胶跑道上阶梯式增加跑程的测试方法.在测试过程中采用通用计量程序.在冰上进行专项测试时,让冰球运动员做54米×6(从一球门线到另一球门线)的往返跑.用这种方法来测定冰球运动员的无氧耐力.在做往返跑的过程中没     

运动员维生素供应状况的评定

现代大量实验资料证明,在完成大运动量及高强度负荷时,机体对维生素的需求量增加,其中主要有维C、维B_1、维B_2、维P等。在运动实践中,特别是在比赛前和比赛期,所增加的需求量是靠补充每天的摄入量来实现的,并没有考虑人体中维生素状态的特殊性,既补充单一品种维生素,又补充各种复合维生素。我们的研究目的是在准备比赛的最后阶段,在出现缺乏维生素的情况下,用专门食品作     

Dielectrophoretic differentiation of mouse ovarian surface epithelial cells, macrophages, and fibroblasts using contactless dielectrophoresis

Ovarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a non-tumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool.     

Mapping of hyperthermic tumor cell death in a microchannel under unidirectional heating

Hyperthermia can be used as an adjunctive method of chemotherapy, radiotherapy, and gene therapy to improve cancer treatment. In this study, we investigate the hyperthermic cell death of cervix cancer CaSki cells in a microchannel integrated with a directional heating scheme. Heat was applied from the inner end to the outer end of the channel and a temperature distribution from 60 °C to 30 °C was established. A three dimensional (3D) numerical model was conducted for the heat transfer simulation, based on which a simple fitting method was proposed to easily estimate the temperature distribution along the channel. Cell death along the channel was mapped 22 h after the heating treatment by dual fluorescent labeling and phase-contrast microscopy imaging. Upstream, where the temperature is higher than 42 °C, we observe necrotic death, late-stage and early stage apoptotic death in sequence along the channel. Downstream and in the middle of the channel, where the temperature is lower than 42 °C, significant cell detachment was noted. Vigorous detachment was observed even in the non-hyperthermic zone (temperature lower than 37 °C), which we believe is due to the direct effect of the hyperthermic zones (higher than 37 °C). The present work not only gives a vivid map of cell responses under a temperature gradient, but also reveals the potential interactions of the heated tumor cells and non-heated tumor cells, which are seldom investigated in conventional petri-dish experiments.     

Gravity-oriented microfluidic device for uniform and massive cell spheroid formation

We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours.     

The effects of Ramadan intermittent fasting on athletic performance: recommendations for the maintenance of physical fitness

The behavioural modifications that accompany Ramadan intermittent fasting (RIF) are usually associated with some alterations in the metabolic, physiological, and psychological responses of athletes that may affect sport performance. Muslim athletes who are required to train and/or compete during the month-long, diurnal fast must adopt coping strategies that allow them to maintain physical fitness and motivation if they are to perform at the highest level. This updated review aims to present the current state of knowledge of the effects of RIF on training and performance, focusing on key-factors that contribute to the effects of Ramadan on exercise performance: energy restriction, sleep deprivation, circadian rhythm perturbation, dehydration, and alterations in the training load. The available literature contain few studies that have examined the effects of RIF on physical performance in athletes and, to date, the results are inconclusive, so the effects of RIF on competition outcomes are not at present wholly understood. The diverse findings probably indicate individual differences in the adaptability and self-generated coping strategies of athletes during fasting and training. However, the results of the small number of well-controlled studies that have examined the effects of Ramadan on athletic performance suggest that few aspects of physical fitness are negatively affected, and where decrements are observed these are usually modest. Subjective feelings of fatigue and other mood indicators are often cited as implying additional stress on the athlete throughout Ramadan, but most studies show that these factors may not result in decreases in performance and that perceived exercise intensity is unlikely to increase to any significant degree. Current evidence from good, well-controlled research supports the conclusion that athletes who maintain their total energy and macronutrient intake, training load, body composition, and sleep length and quality are unlikely to suffer any substantial decrements in performance during Ramadan. Further research is required to determine the effect of RIF on the most challenging events or exercise protocols and on elite athletes competing in extreme environments.     

Achieving optimum sports performance during Ramadan: some practical recommendations

Muslim athletes should fast from sunrise to sunset each day throughout the 30 days of Ramadan. Most athletes will continue to train throughout Ramadan, and they may also be required to compete at this time, but they will also engage in the religious, cultural, and social activities that Ramadan represents. The available evidence indicates that high-level athletes can maintain performance during Ramadan if physical training, food and fluid intake, and sleep are appropriate and well controlled. Individualized monitoring of athletes may help to prevent fatigue and overtraining and to reduce the risk of consequent illness and injury. The timing and intensity of training may require adjustment to optimize the training response, and training close to or after sunset may have advantages, but this will vary between individual and team sports and between environments that are predominantly Muslim and those that are predominantly non-Muslim. Training late in the day allows nutrition interventions after training to promote adaptations to the training stimulus, to promote recovery, and might help to reduce muscle damage. Sleep deficits have a number of adverse effects on well-being and performance, and athletes should ensure adequate sleep throughout Ramadan. In non-Muslim majority environments, especially in team sports, coaches and athletes should be sensitive to the needs of their team-mates who may be fasting. Event organizers should take account of the needs of Muslim athletes when scheduling the dates and timings of sports competitions.     

African American preschool children's physical activity levels in Head Start

The purpose of this study was to describe the physical activity levels of urban inner city preschoolers while attending Head Start, the federally funded preschool program for children from low-income families. Participants were 158 African American children. Their physical activity during Head Start days was measured using programmed RT-3 accelerometers. Results revealed that the children spent the most time in sedentary and light physical activity, while their participation in moderate-to-vigorous physical activities was low. Given the sedentary class format and limited physical space for the Head Start programs observed, we suggest adding a structured physical activity component to Head Start schools to fight the overweight and obesity crisis.     

Calling controversy: assessing synthetic biology's conflict potential

Will synthetic biology elicit controversies similar to those of genetically modified crops before? Maybe, but where exactly are the analogies, and how can we gain substantial insights rather than mere guesses? We argue that as well as the intrinsic properties of the technologies at stake, the context of their implementation is decisive. To assess mechanisms of past and potential controversies, an investigative tool is presented. The Gate Resonance model, derived from older models of societal conflict, allows the identification of key elements of conflict generating processes. In monitoring the developing debate on synthetic biology using this model, analogies to the case of genetically modified crops appear less convincing. So far, there are only few indications that a controversy is imminent.