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Puchberger-Enengl D Podszun S Heinz H Hermann C Vulto P Urban GA 《Biomicrofluidics》2011,5(4):044111-044111-10
In this contribution, we present a system for efficient preconcentration of pathogens without affecting their viability. Development of miniaturized molecular diagnostic kits requires concentration of the sample, molecule extraction, amplification, and detection. In consequence of low analyte concentrations in real-world samples, preconcentration is a critical step within this workflow. Bacteria and viruses exhibit a negative surface charge and thus can be electrophoretically captured from a continuous flow. The concept of phaseguides was applied to define gel membranes, which enable effective and reversible collection of the target species. E. coli of the strains XL1-blue and K12 were used to evaluate the performance of the device. By suppression of the electroosmotic flow both strains were captured with efficiencies of up to 99%. At a continuous flow of 15 μl/min concentration factors of 50.17 ± 2.23 and 47.36 ± 1.72 were achieved in less than 27 min for XL1-blue and K12, respectively. These results indicate that free flow electrophoresis enables efficient concentration of bacteria and the presented device can contribute to rapid analyses of swab-derived samples. 相似文献
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Heinz Götze 《Publishing Research Quarterly》1997,13(1):52-72
Adapting to market changes after World War II, the German scientific publishing house Springer-Verlag decided to publish in
the English language long before setting up offices in the United States.
This material was adapted from Springer-Verlag: History of a Scientific Publishing House, Volume 2, 1945–1992, and appears with the permission of the publishers. 相似文献
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Heinz Draheim 《European Journal of Engineering Education》1981,6(3-4):203-206
“The Technical University of Budapest and the University of Karlsruhe, guided by a common desire to promote mutual academic and cultural relations and convinced that cooperation in teaching and research will be mutually beneficial, have agreed to the following:
that both universities declare their desire to institute and promote academic and cultural cooperation; that the areas and structure and the means for cooperation shall be decided by mutual consent.” 相似文献
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Should gifted and high-achieving students cooperate in homogeneous groups or do they learn better individually? Or should they learn together with less gifted peers in heterogeneous groups? Current empirical research is reviewed in this article and meta-analyses of available results in 12 published studies are performed. Results show that few methodologically sound studies can be found at present. In addition, researchers only considered a restricted spectrum of participants, focused on learning of relatively simple materials, measured only a restricted range of possible outcomes, and neglected processes and activities of the learners, which might determine outcomes of cooperative learning. Results therefore remain controversial even for fundamental issues like learning in homogeneous versus heterogeneous, mixed-ability groups. In spite of these limitations, analyses nevertheless show that cooperative learning offers strong potentials for further improving the quality of instruction with gifted and high-achieving students. Further empirical research is required to obtain more differentiated information on the various aspects of cooperative learning. 相似文献
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Diversifying the teaching population is of international concern. Ireland has experienced significant socio-demographic change in the last decades, but we lack adequate data on the backgrounds of student teachers, especially in relation to nationality and ethnicity. In this paper, we examine the nationality/ies, ethnicity/ies and experiences with diversity of Ireland’s 2014 undergraduate primary and postgraduate post-primary initial teacher education (ITE) applicants, entrants and non-entrants, part of the focus of the Diversity in Initial Teacher Education in Ireland national research project. The overwhelming majority of respondents claim Irish only nationality, and are of ‘White Irish’ ethnicity. Respondents’ constructions of their ethnicity privilege nationality and skin colour. The exclusionary constructions of ethnicity of those self-identifying as ‘White Irish’ reveal the heavy influence of Ireland’s post-colonial nationhood. A significant proportion reported having little or no experience with groups different to their own. We end by noting implications on policy, research and ITE programme levels. 相似文献