Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways

Mesenchymal stem cell(MSC)transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium,but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment.The aim of this study is to explore the cytoprotection of heat shock protein 90(Hsp90)against hypoxia and serum deprivation-induced apoptosis and the possible mechanisms in rat MSCs.Cell viability was determined by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis was assessed by Hoechst 33258nuclear staining and flow cytometric analysis with annexin V/PI staining.The gene expression of Toll-like receptor-4(TLR-4)and V-erb-b2 erythroblastic leukemia viral oncogene homolog 2(ErbB2)was detected by real-time polymerase chain reaction(PCR).The protein levels of cleaved caspase-3,Bcl-2,Bcl-xL,Bax,total-ERK,phospho-ERK,totaI-Akt,phospho-Akt,and Hsp90 were detected by Western blot.The production of nitric oxide was measured by spectrophotometric assay.Hsp90 improves MSC viability and protects MSCs against apoptosis induced by serum deprivation and hypoxia.The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways,but also enhances the paracrine effect of MSCs.These findings demonstrated a novel and effective treatment strategy against MSC apoptosis in cell transplantation.     

Neurochip based on light-addressable potentiometric sensor with wavelet transform de-noising

Neurochip based on light-addressable potentiometric sensor(LAPS),whose sensing elements are excitable cells,can monitor electrophysiological properties of cultured neuron networks with cellular signals well analyzed.Here we report a kind of neurochip with rat pheochromocytoma(PC12) cells hybrid with LAPS and a method of de-noising signals based on wavelet transform.Cells were cultured on LAPS for several days to form networks,and we then used LAPS system to detect the extracellular potentials with signals d...     

Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients

Objective  

The purpose of this study was to determine the unique and universal features of microsatellite instability-high (MSI-H) colorectal cancer (CRC) and MSI-H gastric cancer (GC) in the Chinese population.     

A facile approach to construct hybrid multi-shell calcium phosphate gene particles

The calcium phosphate (CaP) particles have attracted much attention in gene therapy. How to construct stable gene particles was the determining factor. In this study,hybrid multi-shell CaP gene particles were successfully constructed. First,CaP nanoparticles served as a core and were coated with DNA for colloidal stabilization. The ξ-potential of DNA-coated CaP nanoparticles was -15 mV. Then polyethylenimine (PEI) was added and adsorbed outside of the DNA layer due to the electrostatic attraction. The ξ-pot...     

利用GSM短消息实现热计量表远程无线抄表系统的设计

针对居民供暖系统采用分户控制、分户计量之后带来的抄表问题,提出利用GSM短消息设计热计量表远程无线抄表系统的方案.给出了系统的总体设计思路以及具体的软硬件设计方案,通过该系统实现了热计量表的远程无线抄表.     

中国法制史课程网上教学考核方案的实践与探析

为了适应＂中央广播电视大学人才培养模式改革和开放教育试点＂课程考核改革的需要,中国法制史课程网上教学考核方案的设计,充分考虑了开放教育学生在职学习的客观情况和本门课程的特点,力求把教、学、评紧密地结合起来,并在以滁州电大在线平台为中介的教学互动过程中,形成师生之间＂以教促学＂和＂以考促学＂的良性循环。使学生学习由考前突击向注重平时学习转变,由被动学习向主动学习转变,从而提高学生自主学习能力,达到提高教学质量的目的。通过形成性考核和终结性考试相结合的方式,全面检测评价学生的学习过程、学习行为和学习成果,促进学生学习目标的实现。     

用实数完备性证明闭区间上连续函数的有界性

用实数完备性定理（区间套定理、确界原理、单调有界定理、柯西收敛准则）,直接证明了闭区间上连续函数的有界性,从一侧面反映了实数完备性的6个基本定理是互相等价的。     

关于高校旅游专业基础课教学两难境地的思考

旅游专业的综合性使得专业基础课的教学面临基础学科与专业特色不能很好融合的矛盾,教学陷入两难境地。本文深入分析了这种两难困境产生的原因,并提出解决建议。     

新时期高校贫困大学生问题研究

本文主要分析贫困大学生现状,然后从工作机制、思想政治教育等方面提出加强贫困大学生教育问题的对策。     

克利姆特的绘画语言在加利亚诺服装设计中的应用

从点、线、面、色彩、装饰等方面入手,分析加利亚诺的服饰设计作品,解读其中蕴含的克利姆特绘画的精髓。加利亚诺延续了克利姆特形式多样的点、线、面,鲜艳的色彩,唯美的装饰。同时加入大量的金属片、宝石以加强点的效果,辅以鲜艳的色彩使得线更加生动,采用廓形设计突出面的立体效果,结合现代工艺和自然元素营造更加独特的装饰风格。对于克利姆特的绘画艺术,加利亚诺在吸收的基础上,完美地升华,将平面转化为立体,将意象转化为具象．将造型转化为服装。