Development and fertility studies on post-bio-electrosprayed Drosophila melanogaster embryos

Bio-electrosprays (BESs) provide a means of precisely manipulating cells and thus have the potential for many clinical uses such as the generation of artificial tissues∕organs. Previously we tested the biological safety of this technology with a variety of living cells and also embryos from the vertebrate model organisms Danio rerio (zebrafish) and Xenopus tropicalis (frog). However, the viability and fertility of the treated embryos could not be fully assessed due to animal licensing laws. Here we assay the viability and fertility of Drosophila melanogaster (fruit fly) embryos in conjunction with the bio-electrospray procedure. Bio-electrosprayed Drosophila embryos developed into fully fertile adult flies that were indistinguishable from wild-type. Thus, we demonstrate that the bio-electrospray procedure does not induce genetic or physical damage that significantly affects the development or fertility of a multicellular organism. This study along with our previous investigations demonstrates the potential of this approach to be developed for the precise manipulation of sensitive biological materials.     

Role of joint torques generated in an optimised Yurchenko layout vault

The purpose of the study was to quantify the muscle torques required in the performance of an optimised Yurchenko layout vault based on a five-segment rigid link model and using input data from an elite female gymnast. At impact, the wrist torque trajectory indicated an extension-flexion action while the shoulder was characterised by extension. The approximate 100 Nm (wrist flexor) and 125 Nm (shoulder extensor) respective peak torque magnitudes indicated that the impact action is not passive in nature. The contribution of joint torques to the adjoining segments was apportioned to the relative components namely; centripetal, gravity and net joint torque components. Despite the presence of both large wrist and shoulder joint torques, the net turning effect on the upper limb and hand segments about their centre of mass (CM) was small. The principal role of the upper limb joint torques was therefore to effect the appropriate joint motions and to support the weight of the gymnast. The performance of the optimum vault was primarily the result of the interplay between the centripetal and the net joint torque components at the wrist, hip and shoulder joints. This has implications to the performer in that successful execution of the vault is principally concerned with the ability to create a high angular momentum for horse impact and to then apply an appropriate level of joint torques that will make optimal use of the initial kinetic condition.     

Adoptive Gay Father Families: Parent–Child Relationships and Children's Psychological Adjustment

Findings are presented on a U.K. study of 41 gay father families, 40 lesbian mother families, and 49 heterosexual parent families with an adopted child aged 3–9 years. Standardized interview and observational and questionnaire measures of parental well‐being, quality of parent–child relationships, child adjustment, and child sex‐typed behavior were administered to parents, children, and teachers. The findings indicated more positive parental well‐being and parenting in gay father families compared to heterosexual parent families. Child externalizing problems were greater among children in heterosexual families. Family process variables, particularly parenting stress, rather than family type were found to be predictive of child externalizing problems. The findings contribute to theoretical understanding of the role of parental gender and parental sexual orientation in child development.