排序方式: 共有41条查询结果,搜索用时 31 毫秒
21.
Even though isoelectric focusing (IEF) is a very useful technique for sample concentration and
separation, it is challenging to extract separated samples for further processing. Moreover, the
continuous sample concentration and separation are not possible in the conventional IEF. To overcome
these challenges, free flow IEF (FFIEF) is introduced in which a flow field is applied in the
direction perpendicular to the applied electric field. In this study, a mathematical model is developed for FFIEF to understand
the roles of flow and
electric fields for
efficient design of microfluidic
chip for continuous separation of proteins from an initial well mixed solution. A finite volume based
numerical scheme is implemented to simulate two dimensional FFIEF in a microfluidic chip. Simulation results
indicate that a pH gradient forms as samples flow downstream and this pH profile agrees well with
experimental results validating our model. In addition, our simulation results predict the experimental behavior of
pI markers in a FFIEF microchip. This numerical model is used to predict the separation
behavior of two proteins
(serum albumin and cardiac troponin I) in a two-dimensional straight microchip. The effect of
electric field is
investigated for continuous separation of proteins. Moreover, a new channel design is presented to increase the separation
resolution by introducing cross-stream flow velocity. Numerical results indicate that the separation
resolution can be improved by three folds in this new design compare to the conventional straight
channel design. 相似文献
22.
Allan B. de Guzman Hye-Eun Shim Charmin Kathleen M. Sia Wilbart Harvey S. Siazon Mary Joyce Ann P. Sibal Joanna Brigitte Lorraine C. Siglos 《Educational gerontology》2013,39(4):265-291
Ego integrity, the last developmental task in Erikson's psychological theory, develops naturally among older people. However, the presence of loss–like physical disability–can considerably affect the quality of life, interactions, and well being of older adults. Hence, older people with physical disabilities need more assistance in accomplishing tasks of ego integrity. This study aimed to utilize traditional Filipino art to capture the essence of the lived experiences of a select group of older people with physical disabilities in relation to their ego integrity. Four Filipino elderly with physical disabilities were purposely selected for this study. Picture prompting and doodling sessions were held as pretest and posttest to ascertain the effectiveness of puni-making in facilitating ego integrity of participants. A series of interviews were pursued to elicit experiences and feelings of participants regarding their disability and old age. From the analysis of the triangulated set of data, two findings emerged. First, four activators of ego integrity, namely: work, family, belief in self and belief in God, were identified as essentials in the formation of the participants' ego-integrity. Second, shifts related to the activators were identified indicating ego integrity's source, meaning and connection elements have become more grounded, certain and integrated. The influence of puni-making in understanding the ego integrity of older people with disabilities was found to be a sound and novel recreational therapy. It can stimulate a select group of Filipino elderly for openness to share their subjective and yet illuminating life experiences. 相似文献
23.
Jenna Min Shim 《课程研究杂志》2013,45(6):739-758
At the current historical juncture in which differences and inequalities are surfacing greater than ever in the world, societies, and schools, the main goal of this essay is to revisit the aspects of structuralism that can potentially contribute productively to understanding the invisible structures and forces that everyone carries (mostly unknowingly) with them at all times and in all places including in multicultural curricular and social justice work. By doing so, this essay also attempts to dispute with liberal humanistic notions of the self (teachers and students) in multicultural curricular studies that continues to support the dominant framework of curriculum. This essay is grounded on the idea that progressive change is predicated on (although never guaranteed by) uncovering and understanding as fully as possible the social, political, and economic organization of the world, which is always mediated by individual selves who are located within the world in specific ways. 相似文献
24.
Minsun Shim 《亚洲交流杂志》2015,25(1):48-64
Much research on risk perception and health behavior has examined cognitive dimensions of risk but not affective dimensions. To address this gap, this study examines both cognitive risk perception (perceived risk of susceptibility and severity) and affective risk perception (worry) in the context of food safety risks in East Asia. We investigate their roles in independently and jointly predicting intention to consume outbreak-associated food products, as well as mediating the influences of news exposure and attention on intention. Data from a nationwide survey in South Korea (N = 1500) lent overall support for our hypotheses in both cases of processed food from China and seafood from Japan. Our findings show: (1) both perceived risk and worry were negatively associated with food consumption intention, and the association between perceived risk and intention was stronger among those higher in worry; (2) news attention had stronger associations with perceived risk and worry than news exposure, and attention moderated the relationship between news exposure and perceived risk; and (3) perceived risk and worry mediated the associations between news use and food consumption intention. Implications and limitations of the findings are discussed. 相似文献
25.
Evaluation of a lower-body compression garment 总被引:1,自引:0,他引:1
Doan BK Kwon YH Newton RU Shim J Popper EM Rogers RA Bolt LR Robertson M Kraemer WJ 《Journal of sports sciences》2003,21(8):601-610
The aims of this study were to determine how custom-fit compression shorts affect athletic performance and to examine the mechanical properties of the shorts. Ten male and 10 female track athletes on a university's nationally competitive track team, specializing in sprint or jump events, participated in the study. Testing utilized the compression shorts with loose-fitting gym shorts as the control garment. Several significant effects were revealed for the custom-fit compressive garment. Although 60 m sprint time was not affected, hip flexion angle was reduced. Skin temperature increased more and at a faster rate during a warm-up protocol. Muscle oscillation was decreased during vertical jump landing. Countermovement vertical jump height increased when the participants were wearing the custom-fit compression garment. In materials testing, the elasticity of the compressive garment provides increased flexion and extension torque at the end range of extension and flexion, respectively, and may assist the hamstrings in controlling the leg at the end of the swing phase in sprinting. The compressive garment significantly reduced impact force by 27% compared with American football pants alone. Through various mechanisms, these findings may translate into an effect on athletic performance and a reduction in injuries. 相似文献
26.
Seung-hwan Shim 《Educational Philosophy and Theory》2020,52(9):973-985
AbstractThis study explores the views of death in the ideas of Kierkegaard and Heidegger to discuss the educational meaning of death and the direction of death education. What both thinkers have in common is, first, that death is not universal, but that each individual is independently aware of his or her own death. Second, both thinkers observe that we should face death and suffer from anxiety or despair, a kind of existential limitation, which serves as an opportunity for real existence. Third, both thinkers observe that individuals can transform their lives and create an opportunity to renew their relationships with people or things around them through their existential insights into death. Death education based on the viewpoints of the two thinkers should first awaken each individual to his or her own authenticity and individuality by means of the associative perception of life and death. Second, each student should actively face the ‘crisis of death’ without circumventing it or escaping from it, thus, learn to have a sincere attitude toward one’s own life and relationships with others. To this end, it is necessary to use various educational materials related to death for regular subject curriculum and non-subject experience activities to induce students to ask existential questions and to reflect on them on their own. 相似文献
27.
Jenna Min Shim 《课程研究杂志》2017,49(6):830-847
Reading psychoanalytic theory of the unconscious desire for wholeness in the light of the notion of white racial supremacy, this study explores a constituted difficulty that self-reflection around the issues of race and racism confronts by exploring three white male pre-service teachers’ emotional experiences inscribed in their responses to the process of engaging in self-reflection. The pervasiveness of strong emotional reactions to the process of engaging in self-reflection spoke volumes not only about how uncomfortable the participants felt in the face of ambivalence, incommensurability and vulnerability but also about their active resistance against their emotional states to defend their unconscious desire for wholeness that is intensified by the forces of white racial supremacy. This study also seeks to elaborate problems inherent in teachers’ self-reflection and why and how unknown and unconscious emotional world filters and at times hinders the participants’ willingness to inquire into their own relationship to race and racism. In the final section, the author discusses how the findings may be used to imagine how teachers’ unknown and unconscious emotional world can become subjective curriculum which in turn becomes a foundation for students’ learning or lack thereof and also informs the field of teacher education engaging in critical self-reflection. 相似文献
28.
This study proposes the ideal role of teachers through the examination of Plato, Confucius, Buber, and Freire on the subject. Teachers not only contribute to the development of individuals and societies but also attain self-realization through teaching. As such, the role of teachers is important as a goal as well as a means. To examine such role, this study selects four major approaches to understanding teaching. Plato regards teaching as guidance into objective knowledge through the reasoned understanding of causes. Confucius regards teaching as leading self-cultivation. Buber sees the role of teachers as building a relationship with their students. Freire focuses on critical consciousness towards oppressed situations. This study regards the role of teachers as a complex of various aspects. Thus, it tries to show a more complete understanding of the role of teachers through the synthesis and comparison of these four aforementioned approaches. 相似文献
29.
Gyoungho Lee Jaesool Kwon Sang‐Suk Park Jung‐Whan Kim Hyeok‐Gu Kwon Hac‐Kyoo Park 《科学教学研究杂志》2003,40(6):585-603
Based on conceptual change theory, cognitive conflict is known as an important factor in conceptual change even though there are still questions about its positive and negative effects on science learning. However, there is no reliable method by which to assess the cognitive conflict students experience in their learning. The purpose of this research was to develop an instrument for measuring secondary students' cognitive conflict levels as they learned science. The results of this study indicate that our instrument is a valid and reliable tool for measuring cognitive conflict levels. Factor analysis supported the model that cognitive conflict consists of four constructs: recognition of an anomalous situation, interest, anxiety, and cognitive reappraisal of the conflict situation. Implications for instruction and possibilities for future research are discussed. © 2003 Wiley Periodicals, Inc. J Res Sci Teach 40: 585–603, 2003 相似文献
30.
Seung-hwan Shim 《Religious education (Chicago, Ill.)》2017,112(5):557-568
This study will examine the significance of spirituality and how to cultivate it in this modern era, in which introspection on the meaning of life is lacking due to issues including consumerism, violence, terror, hedonism, and the influences of an instrumental education environment, through Augustine's Confessions. This study suggests the significance of spiritual education as the sharing of stories of spiritual life to sublimate the joy of spiritual learning achieved after overcoming vulnerability and limitations as a human to the joy of sharing and teaching through Confessions, by Augustine. 相似文献