Anti-angiogenesis effect of generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide on breast cancer in vitro

Objective: To study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAMNEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells. Methods: We examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells. Results: The compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly. Conclusion: With a low toxicity, high safety, and high transfection rate, G4PAMAMNEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies.     

论孔子哲学思想的天命鬼神观

孔子的天命鬼神观思想一直是学术界争论不休的一个问题,这一思想在孔子的哲学思想中也占据着重要的地位。文章主要从孔子对“天”、“命”、“天命”的理解和对“鬼神”的态度;“知命”、“顺命”、“乐天”和“知其不可为而为之”的关系;天命鬼神观所体现出的注重人事和重视现实的精神;天命鬼神现在孔子学说中的地位及其影响四个方面试图全面而又在最大程度上认清并分析孔子的天命鬼神观。     

元代神仙道化剧的道家情怀及其文化基础(上)

元杂剧中通过美化神仙和道教境界的方式来否定现实人生的剧本可称之为神仙道化剧.儒家和道家政治思想和政治精神就作为社会政治和人生理想的传统而被后来人分别继承发扬.元代杂剧在对这两种文化精神的体现和发扬中,更倾向于道家人文情怀.道家情怀展现在文学中,常常体现为自然潇散的人生形式和超然忘我的人生境界,以及由此化生出的混同自然、任性无为、超然不受物累的生命态度,这一倾向在神仙道化剧中体现更突出,而许多人神恋爱方式的剧作所表达的倾向则大多是绝意尘世归依自然,其中主要是对自然的美和隐逸生活加以称赞,并通过这赞美使人世的繁华暗然失色.     

元代神仙道化剧的道家情怀及其文化基础（下）

元杂剧中通过美化神仙和道教境界的方式来否定现实人生的剧本可称之为神仙道化剧.儒家和道家政治思想和政治精神就作为社会政治和人生理想的传统而被后来人分别继承发扬.元杂剧在对这两种文化精神的体现和发扬中,更倾向于道家人文情怀.道家情怀展现在文学中,常常体现为自然潇散的人生形式和超然忘我的人生境界,以及由此化生出的混同自然、任性无为、超然不受物累的生命态度,这一倾向在神仙道化剧中体现更突出,而许多人神恋爱方式的剧作所表达的倾向则大多是绝意尘世归依自然,其中主要是对自然的美和隐逸生活加以称赞'并通过这赞美使人世的繁华暗然失色.     

Morphology evolution of two-phase Cu-Ag alloys under different conditions

Cu-Ag filamentary microcomposites with different Ag contents were prepared by cold drawing and intermediate heat treatments. The microstructure characterization and filamentary distribution were observed for two-phase alloys under different conditions. The effect of heavy drawing strain on the microstructure evolution of Cu-Ag alloys was investigated. The results show that the microstructure components consist of Cu dendrites, eutectic colonies and secondary Ag precipitates in the alloys containing 6%-24% （mass fraction） Ag. With the increase in Ag content, the eutectic colonies in the microstructure increase and gradually change into a continuous net-like distribution. The Cu dendrites, eutectic colonies and secondary Ag precipitates are elongated in an axial direction and developed into the composite filamentary structure during cold drawing deformation. The eutectic colonies tend to evolve into filamentary bundles. The filamentary diameters decrease with the increase in drawing strain degree for the two-phase alloys, in particular for the alloys with low Ag content. The reduction in filamentary diameters becomes slow once the drawing strain has exceeded a certain level.     

高等农业院校硕士研究生数学课程教学改革方向的研究

文章论述了现行高等农业院校硕士研究生数学课程教学中存在的问题,阐述了数学教学改革的研究动态和数学在农业生产、科学研究中的应用现状,在此基础上,提出了适合创新人才培养的高等农业院校硕士研究生数学课程教学改革的方向。     

二阶心理理论和执行功能在儿童反语理解中的作用

以6-10岁儿童为被试,考察二阶心理理论和执行功能在儿童反语理解中的作用。结果表明,二阶心理理论和执行功能在6-10岁儿童反语理解的不同阶段起不同作用,两者均制约儿童对反语讲话者态度和话语含义的理解,但二阶心理理论还制约儿童对反语现象的解释,执行功能对反语现象的解释无显著影响。     

文化视野中高校思想政治教育的内涵、功能及特征

文化对高校思想政治教育工作具有基础性、战略性意义。在文化裂变图新与整合发展的新世纪,高校必须从文化传播的纬度思考探索思想政治教育工作的内涵、功能和特征,赋予其不凡的时代文化精神。     

城市群众体育锻炼情况调查研究——以石家庄市为例

应用文献资料法、问卷调查法和数理统计法,从锻炼时间、频次、场所、项目、花费、家人态度、锻炼效果、健身指导等方面,对石家庄市区群众参加体育锻炼的情况进行了调查,了解了现阶段城市群众体育锻炼的现状,发现了存在问题,并有针对性地提出了建议。     

傅里叶变换在信号设定门限分解中的应用

针对BRATUMASS系统信号分析中存在的问题,从广义函数中弱函数的Hermite基函数分解出发,对信号的频谱幅度按照某种设定门限序列进行划分,提出对信号分解的方法;还从信号谱幅度概率分布的角度,讨论了确定门限序列下限的方法．从给出的实际信号分解和背景下限确定的过程来看,分离能很好地显示同特性组织回波的合成情况,其提取的下限也能很好地逼近实际的组织特性参数值．