节能服务企业成长动力机制的实证研究

属于生产性服务行业的节能服务企业专业技术性强、智力密集度高,寄望于引领我国节能环保这一战略性新兴产业,其快速成长对于促进节能减排有着重要意义。运用理论建模与数理验证相结合的方法,对动力要素对节能服务企业成长绩效的影响进行实证研究。结果显示,对节能服务企业成长最具显著作用的是支持政策,其次是整合能力、技术人才和资金来源。针对这些研究结果提出相应的对策建议。     

The Undergraduate Research Student Self-Assessment (URSSA): Validation for Use in Program Evaluation

This article examines the validity of the Undergraduate Research Student Self-Assessment (URSSA), a survey used to evaluate undergraduate research (UR) programs. The underlying structure of the survey was assessed with confirmatory factor analysis; also examined were correlations between different average scores, score reliability, and matches between numerical and textual item responses. The study found that four components of the survey represent separate but related constructs for cognitive skills and affective learning gains derived from the UR experience. Average scores from item blocks formed reliable but moderate to highly correlated composite measures. Additionally, some questions about student learning gains (meant to assess individual learning) correlated to ratings of satisfaction with external aspects of the research experience. The pattern of correlation among individual items suggests that items asking students to rate external aspects of their environment were more like satisfaction ratings than items that directly ask about student skills attainment. Finally, survey items asking about student aspirations to attend graduate school in science reflected inflated estimates of the proportions of students who had actually decided on graduate education after their UR experiences. Recommendations for revisions to the survey include clarified item wording and increasing discrimination between item blocks through reorganization.Undergraduate research (UR) experiences have long been an important component of science education at universities and colleges but have received greater attention in recent years, as they have been identified as important ways to strengthen preparation for advanced study and work in the science fields, especially among students from underrepresented minority groups (Tsui, 2007 ; Kuh, 2008 ). UR internships provide students with the opportunity to conduct authentic research in laboratories with scientist mentors, as students help design projects, gather and analyze data, and write up and present findings (Laursen et al., 2010 ). The promised benefits of UR experiences include both increased skills and greater familiarity with how science is practiced (Russell et al., 2007 ). While students learn the basics of scientific methods and laboratory skills, they are also exposed to the culture and norms of science (Carlone and Johnson, 2007 ; Hunter et al., 2007 ; Lopatto, 2010 ). Students learn about the day-to-day world of practicing science and are introduced to how scientists design studies, collect and analyze data, and communicate their research. After participating in UR, students may make more informed decisions about their future, and some may be more likely to decide to pursue graduate education in science, technology, engineering, and mathematics (STEM) disciplines (Bauer and Bennett, 2003 ; Russell et al., 2007 ; Eagan et al. 2013 ).While UR experiences potentially have many benefits for undergraduate students, assessing these benefits is challenging (Laursen, 2015 ). Large-scale research-based evaluation of the effects of UR is limited by a range of methodological problems (Eagan et al., 2013 ). True experimental studies are almost impossible to implement, since random assignment of students into UR programs is both logistically and ethically impractical, while many simple comparisons between UR and non-UR groups of students suffer from noncomparable groups and limited generalizability (Maton and Hrabowski, 2004 ). Survey studies often rely on poorly developed measures and use nonrepresentative samples, and large-scale survey research usually requires complex statistical models to control for student self-selection into UR programs (Eagan et al., 2013 ). For smaller-scale program evaluation, evaluators also encounter a number of measurement problems. Because of the wide range of disciplines, research topics, and methods, common standardized tests assessing laboratory skills and understandings across these disciplines are difficult to find. While faculty at individual sites may directly assess products, presentations, and behavior using authentic assessments such as portfolios, rubrics, and performance assessments, these assessments can be time-consuming and not easily comparable with similar efforts at other laboratories (Stokking et al., 2004 ; Kuh et al., 2014 ). Additionally, the affective outcomes of UR are not readily tapped by direct academic assessment, as many of the benefits found for students in UR, such as motivation, enculturation, and self-efficacy, are not measured by tests or other assessments (Carlone and Johnson, 2007 ). Other instruments for assessing UR outcomes, such as Lopatto’s SURE (Lopatto, 2010 ), focus on these affective outcomes rather than direct assessments of skills and cognitive gains.The size of most UR programs also makes assessment difficult. Research Experiences for Undergraduates (REUs), one mechanism by which UR programs may be organized within an institution, are funded by the National Science Foundation (NSF), but unlike many other educational programs at NSF (e.g., TUES) that require fully funded evaluations with multiple sources of evidence (Frechtling, 2010 ), REUs are generally so small that they cannot typically support this type of evaluation unless multiple programs pool their resources to provide adequate assessment. Informal UR experiences, offered to students by individual faculty within their own laboratories, are often more common but are typically not coordinated across departments or institutions or accountable to a central office or agency for assessment. Partly toward this end, the Undergraduate Research Student Self-Assessment (URSSA) was developed as a common assessment instrument that can be compared across multiple UR sites within or across institutions. It is meant to be used as one source of assessment information about UR sites and their students.The current research examines the validity of the URSSA in the context of its use as a self-report survey for UR programs and laboratories. Because the survey has been taken by more than 3400 students, we can test some aspects of how the survey is structured and how it functions. Assessing the validity of the URSSA for its intended use is a process of testing hypotheses about how well the survey represents its intended content. This ongoing process (Messick, 1993 ; Kane, 2001 ) involves gathering evidence from a range of sources to learn whether validity claims are supported by evidence and whether the survey results can be used confidently in specific contexts. For the URSSA, our method of inquiry focuses on how the survey is used to assess consortia of REU sites. In this context, survey results are used for quality assurance and comparisons of average ratings over years and as general indicators of program success in encouraging students to pursue graduate science education and scientific careers. Our research questions focus on the meaning and reliability of “core indicators” used to track self-reported learning gains in four areas and the ability of numerical items to capture student aspirations for future plans to attend graduate school in the sciences.     

Breaking the Cycle: Future Faculty Begin Teaching with Learner-Centered Strategies after Professional Development

The availability of reliable evidence for teaching practices after professional development is limited across science, technology, engineering, and mathematics disciplines, making the identification of professional development “best practices” and effective models for change difficult. We aimed to determine the extent to which postdoctoral fellows (i.e., future biology faculty) believed in and implemented evidence-based pedagogies after completion of a 2-yr professional development program, Faculty Institutes for Reforming Science Teaching (FIRST IV). Postdocs (PDs) attended a 2-yr training program during which they completed self-report assessments of their beliefs about teaching and gains in pedagogical knowledge and experience, and they provided copies of class assessments and video recordings of their teaching. The PDs reported greater use of learner-centered compared with teacher-centered strategies. These data were consistent with the results of expert reviews of teaching videos. The majority of PDs (86%) received video ratings that documented active engagement of students and implementation of learner-centered classrooms. Despite practice of higher-level cognition in class sessions, the items used by the PDs on their assessments of learning focused on lower-level cognitive skills. We attributed the high success of the FIRST IV program to our focus on inexperienced teachers, an iterative process of teaching practice and reflection, and development of and teaching a full course.     

The use of economic classifiers for the indexing of scientific publications

The experience from the application of the OKVED 2 economic classifier for indexing scientific publications is considered. More than 9500 articles on the subject of “Automation. Computer Engineering” in authoritative Russian magazines were the material for the study. Some means for the comparative analysis of economic statistics and results of the classification of scientific publications are shown.     

Gay On-Screen: The Relationship Between Exposure to Gay Characters on Television and Heterosexual Audiences' Endorsement of Gay Equality

The current study, grounded in the parasocial contact hypothesis, employed a survey of emerging adults to investigate the relationship between exposure to gay characters on television and heterosexuals' endorsement of gay equality. A positive relationship existed between exposure to on-screen gay characters and gay equality endorsement. The relationship was stronger for racial minority participants than for White participants, and for participants who had no or few interpersonal relationships with openly gay individuals than for participants who had more than three respective relationships. Results are discussed in terms of the parasocial contact hypothesis and television as an agent of social change.     

Shifting the Perspective of the Research Librarian: A CoResearch Paradigm

This article proposes the coresearch engagement model (CoRE), which addresses how the academic research librarian can become a coresearch partner with any patron. The CoRE model is an interpersonal process and is founded upon the concepts of mutual respect, collaboration, reciprocity, and empowerment. Grounded in Howard Gardner’s multiple intelligences theory, Malcolm Knowles’ andragogical model, and guidelines from the Reference and User Services Association, CoRE has the potential to result in engagement for the librarian and patron. The CoRE model can be utilized in many ways including self-awareness and development for those who need and want to expand their abilities as a research librarian. It provides a theoretical framework and a knowledge base, in addition to giving a context for further development by expanding, comparing the related theories for further research, and implementing various applications suggested by this article.     

Thermally multiplexed polymerase chain reaction

Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously—each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel.     

Disrupting the wall accumulation of human sperm cells by artificial corrugation

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very different from that observed in the bulk. Swimming along walls is beneficial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells ∼60 μm long, strongly confined to ∼25 μm shallow chambers. We investigate the nature of the cell trajectories between the confining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confirmed by depth resolved confocal microscopy studies. We show that the introduction of artificial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an effect which we hypothesize may be valuable for microfluidic applications in biomedicine.     

Microfluidic sampling system for tissue analytics

We have developed a microfluidics based sampling system for tissue analytics. The proof-of-concept of the sampling system was demonstrated by extracting lipid samples from tissue biopsies. The sample collection system consists of a disposable silicon based multiport microneedle integrated with polymer microfluidics. The polymethyl methacrylate polymer microfluidic chip has a 10 μl sample reservoir and actuation membranes for liquid pumping. A special automated robotic system was developed to control the positioning of the needle and the sampling procedure on preselected spots on the tissue. Real breast cancer tissue samples were used to test the feasibility of the sampling system. We successfully measured indicative cancer biomarkers from the tissue surface. Phosphatidylcholine and phosphoethanolamine were extracted from the tissue membrane with methyl tert-butyl ether solvent and detected by mass spectrometry. In the future, this tool could be used in characterization of preoperative biopsies and tumour tissues removed during surgery.     

Robust fluidic connections to freestanding microfluidic hydrogels

Biomimetic scaffolds approaching physiological scale, whose size and large cellular load far exceed the limits of diffusion, require incorporation of a fluidic means to achieve adequate nutrient/metabolite exchange. This need has driven the extension of microfluidic technologies into the area of biomaterials. While construction of perfusable scaffolds is essentially a problem of microfluidic device fabrication, functional implementation of free-standing, thick-tissue constructs depends upon successful integration of external pumping mechanisms through optimized connective assemblies. However, a critical analysis to identify optimal materials/assembly components for hydrogel substrates has received little focus to date. This investigation addresses this issue directly by evaluating the efficacy of a range of adhesive and mechanical fluidic connection methods to gelatin hydrogel constructs based upon both mechanical property analysis and cell compatibility. Results identify a novel bioadhesive, comprised of two enzymatically modified gelatin compounds, for connecting tubing to hydrogel constructs that is both structurally robust and non-cytotoxic. Furthermore, outcomes from this study provide clear evidence that fluidic interconnect success varies with substrate composition (specifically hydrogel versus polydimethylsiloxane), highlighting not only the importance of selecting the appropriately tailored components for fluidic hydrogel systems but also that of encouraging ongoing, targeted exploration of this issue. The optimization of such interconnect systems will ultimately promote exciting scientific and therapeutic developments provided by microfluidic, cell-laden scaffolds.