Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

Individual and community practices for constructing communicative resilience: exploring the communicative processes of coping with parental alienation

ABSTRACT

Parental alienation occurs after parents divorce and intentionally or even unintentionally persuade their children to distance themselves from or reject the other parent. Framed by the communication theory of resilience, this study explores the communicative practices that enable and constrain the targeted alienated parents’ ability to create normalcy at the individual and online community levels. We also examine the relationships between individual-level and online community-level resilience. Findings from 40 narrative interviews reveal communicative practices/processes that enable and constrain alienated parents at both the individual and online support group levels. Findings also suggest that there is a robust relationship between resilience practices at the individual and community levels. Theoretical implications and practical applications are discussed.     

Children's Experiences of Bullying Victimization and the Influence on Their Subjective Well-Being: A Multinational Comparison

The study used data from the second wave of the Children's Worlds Survey to explore the relation between children's experiences of bullying victimization (physical and psychological) and their subjective well-being (SWB) across three age groups (8, 10, and 12) and 15 global geographical regions. The sample consists of 47,029 children randomly selected from 15 countries. Data were analyzed using structural equation modeling and multigroup confirmatory factor analysis. The results show appropriate fit structure for the overall model, and that the combined influence of physical and psychological bullying made a significant contribution to SWB across age groups and geographical regions. The findings are interpreted using Cummins's (1995) Homeostasis Theory of SWB.     

Understandingg in terms of information processing

Psychometric g, the general factor in individual differences in all types of tests and performances involving any mental ability, has much wider importance and implications than are encompassed by the field of psychometrics. It is argued that the nature of g must be understood in terms of information processes rather than in terms of the specific knowledge and skills that are seen in the content of conventional mental tests. The wide range of individual differences in g and disparities in the distribution of g in different subpopulations have important implications for understanding some of the major problems confronting public education.     

Research and teaching in the universities of Denmark: Does such an interplay really exist?

The unity of research and teaching is an ingrained principle in university policy in Denmark, but this principle has also been subjected to increasing criticism. This article presents an investigation, consisting of about 50 interviews with teachers from higher educational institutions in Denmark, aiming at the illumination of this issue. According to the conclusions of this investigation it is the basic experience of the teachers that an important interplay exists between research and teaching of the individual teacher, and that almost none would prefer to work in a pure research institution. At the same time the teachers have some crucial reservations. Firstly it can be difficult to administer this twofold task. Secondly the interrelationships are dependent on several factors, the most important of which is the level of teaching and the disciplinary context. This latter factor is explained by differences pertaining to the very nature of varying disciplines - demonstrated and analyzed with reference to Thomas Kuhn and Basil Bernstein. Finally, the importance of the interrelationships between research and teaching for higher educational and research policies in broader perspective is discussed.     

The Viability of Using Rapid Judgments as a Method of Deception Detection

Rapid Judgments (RJs) are quick assessments based on indirect verbal and nonverbal cues that are known to be associated with deception. RJs are advantageous because they eliminate the need for expensive detection equipment and only require minimal training for coders with relatively accurate judgments. Results of testing on two different datasets showed that trained coders were reliably making RJs after watching both long and short interaction segments but their judgments were not more accurate than the expert interviewers. The RJs did not discriminate between truth and deception as hypothesized. This raises more questions about the conditions under which making RJs from verbal and nonverbal cues achieves accurate detection of veracity.