Improvement in the Orthogonal Protein Degradation in Escherichia coli by Truncated mf-ssrA Tag

SsrA peptide tag from Mycoplasma florum has been developed as a versatile biotechnology tool to control orthogonal degradation of tagged proteins in Escherichia coli. Here, using the systematic deletion mutants of mf-ssrA tag, we demonstrated that the residues in two separate regions have different functions in mf-Lon-mediated specific orthogonal target protein degradation in E. coli. The deletion of multiple residues, up to six amino acids, did not fatally abolish its specific degradation activity, instead of being able to improve the stability of the tagged protein in the presence of endogenous proteases before mf-Lon expression in E. coli. Except for previously identified essential residues, the region adjacent to the C-terminal of the mf-ssrA tag was involved in mf-Lon and endogenous protease-mediated degradation. Moreover, the deletion of specific residues made the mf-ssrA tag more effective and compact. The mf-ssrA tag can be implemented in synthetic biology and bioengineering for development of synthetic circuits.     

简析"人的根本就是人本身"对思想政治教育的启示意义

马克思在《〈黑格尔法哲学批判〉导言》中有一段经典论述,这段论述对于揭示思想政治教育的合法性、有效性及根本途径有着重要的启示意义,其中“人的根本就是人本身”是这段论述的核心和精髓,对我们今天的思想政治教育工作富有重大的启示意义。     

《嫉妒》的隐藏艺术

罗布·格里耶的小说《嫉妒》隐藏了许多内容,不仅隐藏了中心人物,还隐藏了最核心的情节,这些都是由小说的叙事方式,由叙述者观察的位置和角度决定的.罗布·格里耶可能要告诉读者:所谓的真实、真相是不存在的,是无法弄清的,不存在传统小说中所谓的真实.     

关于网络教材设计的几点思考

随着现代教育技术的发展与普及，教材设计也开始突破传统模式，与网络、多媒体等技术结合起来。章对网络教材的设计思想、设计原则以及在设计过程中应考虑的一些因素做了分析和讨论，同时，也对网络教材的开发提出了自己的看法和具体的步骤。     

新课改背景下的中学地理"课堂讨论法"

构建开放式地理课程是《全日制义务教育地理课程标准》的一个基本理念,是新课程改革的要求,中学地理课堂讨论的教学方法与野外调查法和多媒体教学法相比,在形式、内容、时间等方面具有很大的优势:课堂讨论与学生的实际生活联系紧密,能让学生积极主动的学习,培养学生具有终身学习的能力及逐步形成正确的世界观、人生观和价值观。     

基于语音识别的汉语发音自动评分系统的初步设计

语音识别技术的发展使得人与计算机的交互成为可能,文章结合了语音识别的相关技术,提出了在对外汉语教学领域中,对汉语自动发音水平评价系统的设计,详细地介绍了系统的结构以及所使用的关键技术。     

Preliminary experimental study on solid-fuel rocket scramjet combustor

Liquid or gaseous fuel scramjet technology has made great progress, and there has been some research attention to solid-fuel scramjet. A new scramjet configuration using solid fuel as propellant, namely solid-fuel rocket scramjet, is tested experimentally. It consists of two combustors. One is a rocket combustor used as gas generator, and the other is a supersonic combustor used for secondary combustion. The experiment simulates a flight Mach number of 4 at high altitude (stagnation temperature and pressure are 1170 K and 1.16 MPa, respectively), and metalized solid fuel is used as propellant. The results reveal that fuel-rich gas from the gas generator can burn with air in the supersonic combustor. Preliminary evaluation results show that the combustion efficiency of the propellant is about 90%, and the total pressure recovery coefficient in the supersonic combustor is about 0.6. These results indicate that the configuration of solid-fuel rocket scramjet is feasible.     

提高教师继续教育效果的几点思考

教师继续教育对提高中小学教师素质有着重要意义,提高教师继续教育效果可从三个方面努力:合理确定教师继续教育的目标;丰富培训形式,使教师继续教育灵活多样;打造核心竞争力,彰显培训机构资源整合力。     

抱怨信函中的主位推进

文章主要通过系统功能语法中的主住推进模式，对主住推进在外贸信函——抱怨信函中的应用情况进行分析和探讨，并总结出了其使用特点。     

孔子的礼法思想

先秦时代的礼是那个时代的法。从《左传》等文献中,我们看到了它的存在;再从孔子对于礼的论述中,我们明白了礼在那个时代就是客观存在着的法。礼法不仅限制人们的外在行为,而且约束人们的思想。冠礼是对成年男子的责任教育,婚礼是对妇女进行贞节和柔顺的教化,祭礼是对整个社会的周密设计和对所有的人的精心安排。孔子礼法思想深刻而普遍地影响着中国人。