面向东盟的物流专业教学改革研究与实践

本文就目前东盟物流及广西物流的发展现状分析现代物流对课程教学的要求,提出面向东盟的现代物流专业人才教学模式,在课程内容上,设置具有地方特色的课程;在教学方式上,提出“竞赛式课堂”的教学模式,通过改革,培养适应本土经济发展的区域性物流人才。     

高校材料类专业选修课的教学改革与探索

本文以材料专业选修课为例,从学校管理、教师和学生几个方面分析了专业选修课教学过程中存在的问题,并针对存在的问题进行了研究和探索,提出相应的建议和改进措施。     

骨科临床见习教学模式转变的探索与创新

探索骨科临床见习教学模式新方法．让见习生提高临床实践操作能力,临床分析能力,解决临床问题的能力以及树立见习生正确的医德医风．促进见习生学习的积极性、主动性。     

An augmented Lagrangian algorithm for total bounded variation regularization based image deblurring

The augmented Lagrangian strategy has recently emerged as an important methodology for image processing problems. In this paper, based on this strategy, we propose a new projected gradient algorithm for image deblurring with total bounded variation regularization. The convergence property of our algorithm is discussed. Numerical experiments show that the proposed algorithm can yield better visual quality than the Rudin–Osher–Fatemi (ROF) method and the split Bregman iteration method.     

经济与环境协调发展的模型分析与计量检验

为了分析经济与环境间能否实现协调发展,本文首先将资源环境因素纳入内生经济增长模型,运用最优控制方法得出了可持续发展能否实现以及实现条件。在此基础上,以我国1995-2011年30个省的CO2排放为例,通过构建联立方程模型和运用3SLS法对EKC假说进行了计量检验。结果表明:(1)当消费跨期替代弹性等满足一定条件时,即可实现经济与环境间的协调发展;(2)虽然现阶段经济增长与CO2排放呈正向变动,但EKC假说仍是成立的,即我国最终能实现两者间的协调发展;(3)工业结构、贸易开放度和城市化水平对碳排放不利,能源强度、一次能源消费结构和环境规制则正好相反,其中能源强度是影响碳排放的关键因素。     

多维异质网络局部性交叉项补偿信道分配算法

在多维异质网络中,由于承载不同的协议和网络,导致信道拥挤和分配不均衡。传统的多维异质网络信道分配算法采用基于信道切换的动态分配算法,在动态切换过程中易导致数据丢失和信道失衡。提出一种基于数据交叉项补偿机制的多维异质网络信道分配算法,进行多维异质网络信道协议分析与模型分析和求解,考虑用户间接收SINR的公平性,构建信道性能因子,引入一个局部性交叉项补偿信道切换中的失衡性实现算法改进。实验结果表明,改进算法使异质网络生命周期得到扩展,有利于数据的准确传输,避免在信道分配动态切换过程中导致的数据丢失和信道失衡,提高了分配效率和准确性,有效地保证了各用户接收SINR的公平性,信道分配性能上优越于传统算法,展示了较好的应用价值。     

杭州市产业集群的知识产权保护机制研究

随着产业集群的不断发展,企业间缺少独立知识产权保护制度。本文以杭州为例,选取了相对典型的传统产业集群和消费品集群进行样本调查及分析,提出了建立行业自律和行政干预相结合的新型知识产权保护制度,对于增强产业核心竞争力,实现产业转型升级具有重要的意义且迫在眉睫。     

A highly parallel microfluidic droplet method enabling single-molecule counting for digital enzyme detection

Although digital detection of nucleic acids has been achieved by amplification of single templates in uniform microfluidic droplets and widely used for genetic analysis, droplet-based digital detection of proteins has rarely been reported, largely due to the lack of an efficient target amplification method for protein in droplets. Here, we report a key step towards digital detection of proteins using a highly parallel microfluidic droplet approach for single enzyme molecule detection in picoliter droplets via enzyme catalyzed signal amplification. An integrated microfluidic chip was designed for high throughput uniform droplet generation, monolayer droplet collection, incubation, detection, and release. Single β-galatosidase (β-Gal) molecules and the fluorogenic substrate fluorescein di-β-D-galactopyranoside were injected from two separated inlets to form uniform 20 μm droplets in fluorinated oil at a frequency of 6.6 kHz. About 200 000 droplets were captured as a monolayer in a capture well on-chip for subsequent imaging detection. A series of β-Gal solutions at different concentrations were analyzed at the single-molecule level. With no enzyme present, no droplets were found to fluoresce, while brightly fluorescent droplets were observed under single-enzyme molecule conditions. Droplet fluorescence intensity distribution analysis showed that the distribution of enzyme molecules under single-molecule conditions matched well with theoretical prediction, further proving the feasibility of detecting single enzyme molecules in emulsion droplets. Moreover, the population of fluorescent droplets increased as the β-Gal concentration increased. Based on a digital counting method, the measured concentrations of the enzyme were found to match well with input enzyme concentration, establishing the accuracy of the digital detection method for the quantification of β-Gal enzyme molecules. The capability of highly parallel detection of single enzyme molecules in uniform picoliter droplets paves the way to microdroplet based digital detection of proteins.     

我国教育经济与管理学科研究全景透视:热点、方法及演变

使用CiteSpace II和NetDraw软件对2001-2010年我国教育经济与管理学科的热点研究领域、方法及演变进行可视化分析,发现该学科以高等教育和基础教育领域为主,对职业技术教育等领域关注不足;形成了教育管理、教育经济和教育政策三个相对独立的研究维度,其中,以问题为导向的教育政策研究正在发挥着学科整合功能;教育经济、教育改革、高等教育及学校管理四个主题成为研究热点,但研究重心呈多元化趋向;主要研究方法逐步从质性向量化演变.     

浅议当前我国高校中大学生感恩教育的现状及对策

感恩是中华民族的传统美德,是高校思想政治教育的重点内容之一,对促进大学生成长成才、形成健康的人生观和道德观有着积极的推动作用。文章通过对当今大学生感恩意识的现状分析,提出社会、家庭、学校三位一体式的感恩教育模式。