Herceptin functionalized microfluidic polydimethylsiloxane devices for the capture of human epidermal growth factor receptor 2 positive circulating breast cancer cells

Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (～80% capture yield) HER2 positive cells from full blood.     

我的生活理念(英文)

Life is like a mulfifacefed prism,which gives different colored images,when viewed from different angles.Look how it appears to different people:     

Reading comprehension interventions for middle school students with learning disabilities: a synthesis of 30 years of research

The authors conducted a synthesis of studies of reading comprehension interventions for middle school students (Grades 6-8) identified with a learning disability. They identified 12 studies between 1979 and 2009 with treatment and comparison designs and 2 single-participant studies. Findings from the studies indicate large effect sizes for researcher-developed comprehension measures. Few studies (n = 4) reported standardized measures of reading comprehension, which indicated medium effect sizes. The majority of study treatments (n = 13) utilized strategy instruction related to main idea or summarization.     

Transient analysis of steam generator in PWR nuclear power plant

ＮｏｍｅｎｃｌａｔｕｒｅＡＡｒｅａ（ｍ２）ＤｈＨｙｄｒａｕｌｉｃＤｉａｍｅｔｅｒ（ｍ）ＥＥｎｅｒｇｙＣｏｎｔｅｎｔ（Ｊ）ｆＰｒｅｓｓｕｒｅＬｏｓｓＣｏｅｆｉｃｉｅｎｔαＶｏｉｄＦｒａｃｔｉｏｎＨＳｐｅｃｉｆｉｃＥｎｔｈａｌｐｙ（Ｊ／ｋｇ）ＩＩｎｅｒｔ...     

电脑杀手(3)

_*-;“;__’__-H;·_:;;_;《____∞“■ih‘;;_;‘#‘_i～早餐时,詹妮发现爸把松饼薄片放进箱中时手不住地上摆动,表情很痛苦。发}生了什我刚刚被烤箱织旅磁雹了一下。会不会是齐佩在烤箱的电线里做了手脚?中午,在学校餐厅里, 艾普詹妮见到了两 丽尔,个伙伴。道格,弃佩可能已经开始向我们报复了。下午放学,詹妮回到家,看见妈妈正用手在厨房的洗涤槽里拨弄着什么。几分神前废物处理器还在运转.呢7妈妈!快把手拿出来!快!一声巨响,废物处理器启动了。再晚___。步,凯乐太太的手就要变成肉酱了。詹妮拨通了艾普丽尔的电话。 电话里传来一个熟悉…     

蘇聯普通學校中的综合技術教育

蘇聯共產黨第十九次代表大會的指示為蘇聯學校開闢了廣闊發展的前途。在普通學校系統中實行綜合技術教育,對於新生一代的教育有特別重大的意義。 綜合技術教育的原則,是馬克思和恩格斯提出來並加以論證的;這一原則,在列寧和斯大林的著作中又得到了進一步的發揮。 馬克思認為,綜合技術教育就是熟悉全部生產過程的基本原理,使少年兒童掌握使用各種簡單生產工具的熟練技巧。 綜合技術教育的原則,是共產主義科學理論的不可分割的一部分,它是在分析了工業發展的客觀過程之後,並根據大工業生產對人們提出的要求而提出來的。綜合技術教育是保證人的各種才能在自由的社會裏能够得到全面發展的重要手段之一。在蘇聯,工業生產正在高度的技術基礎上,在先進的蘇聯科學的各種成就的基礎上,得到不斷的改善。特別是最近幾年來,技術得到了突飛猛進的發展,工業部門得到了新的機器和設備,採用了更加完善的技術操作過程,實行了更加合理的生產組織。