Protein nanotechnology — A powerful futuristic diagnostic technique

Healthcare can be maintained well, when diagnosis is quick, accurate, cost-effective and painless. DNA and RNA based diagnosis may not reveal the right information for certain diseases. Identification and quantification of proteins and their folding mechanism are very important in diagnosis of diseases. Small quantities of proteins, which generally escape from detection and are responsible for the diseases, now can be quantified by protein nanotechniques which aids in the diagnosis. In this review, we have summarized the recent developments in nanotechnologies such as protein microarrays, biosensors etc. and their application in diagnosis of diseases at proteomics level have also been discussed.     

A review of Wolff-Michael Roth’s “In search of meaning and coherence: A life in research”

Wolff-Michael Roth’s In Search of Meaning and Coherence: A Life in Research, presents a selection of his previously published key works layered with current commentary and analysis. In this book review, we explore themes that emerge through the text to trace Roth’s development as a theoretician and as a researcher. We recommend this book for all those who are interested in Wolff-Michael Roth and the trajectory of his career, in particular those who are just beginning their journey towards academic scholarship.

Nanopore Technology: A Simple,Inexpensive, Futuristic Technology for DNA Sequencing

In health care, importance of DNA sequencing has been fully established. Sanger’s Capillary Electrophoresis DNA sequencing methodology is time consuming, cumbersome, hence become more expensive. Lately, because of its versatility DNA sequencing became house hold name, and therefore, there is an urgent need of simple, fast, inexpensive, DNA sequencing technology. In the beginning of this century efforts were made, and Nanopore DNA sequencing technology was developed; still it is infancy, nevertheless, it is the futuristic technology.     

To Establish the Reference Intervals of Lipid Profile in Punjab

Overnight fasting samples of 1,031 apparently healthy people of Punjab visiting the hospital over a period of 3 years were tested for serum lipid profile. The mean ± SD of serum total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol and very low density lipoprotein cholesterol in mg/dl were 182.2 ± 33.9, 122.4 ± 33.4, 44.1 ± 6.8, 113.9 ± 32.0, 24.6 ± 7.1 respectively. When these subjects were grouped according to age and sex, no appreciable difference was observed between most of the groups. Serum triglycerides were found to be low and HDL-C was high in females when compared with males of similar age and vice versa. With advancing age, total cholesterol and low density lipoprotein cholesterol levels were found to be higher in women. The present study suggests that the obtained lipid values should be taken into consideration during clinical evaluation.     

Serum Paraoxonase (PON1) Activity in North-West Indian Punjabi’s with Acute Myocardial Infarction

Human serum paraoxonase-1 (PON1), an enzyme on HDL prevents oxidation of LDL thereby preventing the development of atherosclerosis. Studies done so far have lead to conflicting results. As studies are lacking in North-West Indian Punjabi’s, a distinct ethnic group with high incidence of coronary artery disease, we determined PONase activity in this population. It has been postulated that sudden lowering of serum PONase may lead to precipitation of acute myocardial infarction. We determined serum PONase activity and lipids in 100 patients each of AMI (within 24 h of onset), stable CAD and 100 age and sex matched healthy controls. These were again determined after 6 weeks in AMI patients. The mean serum PONase activity was lowest in AMI patients (23.26 U/ml) followed by stable CAD patients (102.0 U/ml) where as in controls was highest (179.8 U/ml). In patients with AMI, activity was significantly higher at 6 weeks as compared to that after acute event (49.39 %; p < 0.05). Sudden lowering of serum PONase activity in a population which already has lower activity may be one of the risk factors for development of AMI.     

Prediction of vibrations induced by trains on line 8 of Beijing metro

This paper mainly discusses the problem of ground-borne vibrations due to the planned line 8 of Beijing metro which passes under the National Measurement Laboratory. A lot of vibration sensitive equipments are placed in the laboratory. It is therefore necessary to study the impact of vibrations induced by metro trains on sensitive equipments and important to propound a feasible vibration mitigation measure. Based or1 the coupled periodic finite element-boundary element （FE-BE） method, a 3D dynamic track-tunnel-soil interaction model for metro line 8 has been used to predict vibrations in the free field induced by trains running at variable speeds between 30 km/h and 80 km/h. Four types of track structures commonly used on the Beijing metro network have been considered： （1） high resilience direct fixation fasteners, （2） Vanguard fasteners, （3） a floating slab track and （4） a floating ladder track. For each of these track types, the vibration isolation efficiency has been compared. The results of the numerical study can be used to predict vibrations in nearby buildings and to decide upon effective vibration countermeasures.     

Examining the complexities of school-museum partnerships

We examine the research conducted by Kang, Anderson and Wu by discussing it in a larger context of science museum-school partnerships. We review how the disconnect that exists between stakeholders, the historical and cultural contexts in which formal and informal institutions are situated, and ideas of globalization, mediate the success for formal-informal partnerships to be created and sustained.     

Hyperinsulinemia and Hypoadiponectinemia are Associated with Increased Risk for Occurrence of Ovarian Cancer in Non-diabetic Women of North Indian Population

Ovarian cancer has been emerged as a most common and lethal gynecological malignancy in India. High serum insulin and low adiponectin have been associated with increased risk of ovarian cancer. But their role in development of ovarian cancer is conflicting and little evidence is available. We aimed to evaluate blood levels of insulin and adiponectin in epithelial ovarian cancer (EOC) patients and their association with the risk to develop EOC. The study included following three groups; Group 1: fifty cases of cytohistopathologically confirmed cases of EOC, Group 2: fifty age matched cases of benign ovarian conditions and Group 3: fifty ages matched healthy controls with no evidence of any benign or malignant ovarian pathology as ruled out by clinical examination and relevant investigations. Cytohistopathologically confirmed and newly diagnosed cases of EOC and benign ovarian cancer were included in this study. The median value of fasting serum insulin was significantly high (15.0 µlU/ml, P = 0.02) and adiponectin were significantly low (5.1 µg/ml, P < 0.001) in ovarian cancer patients compared to benign ovarian tumors and healthy controls group. A significant increase risk of ovarian cancer was found in high tertile (≥ 18.7 µlU/ml) of serum insulin level (OR = 2.7; 95% CI = 1.00–6.67, P = 0.04) and lower tertile (≤ 5.45 µg/ml) of adiponectin level (OR = 3.2; 95% CI = 1.10–9.71, P = 0.03). High serum insulin level and low adiponectin levels were significantly associated with increased risk for development of ovarian cancer.     

Effect of short term vitamin E supplementation on lipid peroxidation in acute myocardial infarction

In view of sufficient body of evidence to substantiate the role of free radical mediated oxidative injury in myocardial infarction (M.I.), the utility of exogenous supplementation with antioxidant substance, vitamin E was studied. Forty patients of acute myocardial infarction were divided into two equal groups, one who received vitamin E and the other who did not. Plasma MDA levels showed a reduction in patients of M.I. who received vitamin E as well as in those who did not, however, the decrease was significantly more in the vitamin E treated subgroup.