Evaluation of the BD Vacutainer? RST blood collection tube for routine chemistry analytes: clinical significance of differences and stability study

Introduction:

Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer^® Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) blood collection tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer^® RST blood collection tube in reference with the BD Vacutainer^® Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).

Materials and methods:

Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality.

Results:

Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C.

Conclusions:

Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST tube provides shorter clotting time.     

A polystyrene-based microfluidic device with three-dimensional interconnected microporous walls for perfusion cell culture

In this article, we present a simple, rapid prototyped polystyrene-based microfluidic device with three-dimensional (3D) interconnected microporous walls for long term perfusion cell culture. Patterned 3D interconnected microporous structures were created by a chemical treatment together with a protective mask and the native hydrophobic nature of the microporous structures were selectively made hydrophilic using oxygen plasma treatment together with a protective mask. Using this polystyrene-based cell culture microfluidic device, we successfully demonstrated the support of four days perfusion cell culture of hepatocytes (C3A cells).     

中国体育产业上市公司与文化产业上市公司生存状态的比较研究

通过文献资料、数理统计及比较分析等方法,对我国17家体育产业上市公司和35家文化产业上市公司的生存状态进行比较研究。结果显示:2011年中国体育产业上市公司的总体发展规模、盈利能力与投资回报能力较文化产业上市公司仍存在较大差距,但在营运能力和偿债能力方面则比文化产业上市公司更具优势。就各财务指标而言,体育产业和文化产业上市公司的资产规模、利润总额、净利润率、存货周转率及流动比率的区间分布特征较为相似,但两类上市公司的总资产周转率与总资产回报率的分布特征却存在较大差异。体育产业上市公司的净资产收益率、净利润率、每股收益和每股净资产均普遍低于文化产业上市公司,而资产负债率则低于文化产业上市公司。整体来看,文化产业上市公司在多数财务指标上的竞争实力明显强于体育产业上市公司,并且文化产业的规模经济发展效益也显著高于体育产业。     

L型区域特征值问题WILSON元展开及外推的数值实验

文献[1]中对Poisson方程的特征值采用Wilson非协调元进行展开,从误差展开式的形式上推测是下界。利用Wilson元,计算L型区域上的Poisson方程的近似特征值,并对数据进行分析,验证了推测是正确的,然后通过对误差展开式外推,得到了高精度的解。     

“转折”:二年级学生发展的主题词——基于北京高校学生发展调查数据的实证分析

二年级学生发展研究是整个大学阶段学生发展研究的薄弱环节。本研究建构了以独立性形成和目标规划发展为两个维度的二年级学生转折发展指标,通过对北京市22所高校3828名二年级本专科生的问卷调查和相关研究揭示了二年级学生转折发展的情况。研究发现,从高校特征来看,来自民办高校、一般本科院校、工学的男生未完成转折的比例更高;从家庭背景特征来看,来自农村、父亲受教育程度较低的非独生子女未完成转折的比例更高。所以应以这些完成转折有困难的群体为学生工作的主要对象。未完成转折的学生在学业成就和能力发展方面处于劣势地位,而帮助学生完成转折对二年级学生发展具有重要影响,能够显著提高个人学习成绩、促进各方面能力的发展。所以研究提出了高校二年级学生工作开展的具体路径:增加师生之间、同伴之间的互动,提高高校部分学生工作、教学和行政服务工作的质量,让学生更满意,从而有效地帮助他们顺利完成转折。     

中国区域竞技体育发展的外部环境综合测评与分类特征

通过相关文献资料构建我国区域竞技体育发展的外部环境综合评价体系,运用R型聚类、主成分分析及判别分析等多元数理统计方法,对区域竞技体育发展的外部环境进行综合评价与分类。结果显示:区域竞技体育发展的外部环境可归纳为6个维度,全国31个地区竞技体育发展的外部环境可分为5种类型,且第1种类型区域竞技体育发展的外部环境和另外4种类型存在非常明显的差异性。根据各区域竞技体育发展的外部环境特征,提出针对性的建议,以供相关部门决策参考。     

文化教育程度、国民收入水平与体育用品制造业发展的实证研究——基于面板数据的分析方法

基于2003-2009年间省级面板数据设定面板协整模型,实证检验我国居民文化教育程度、人均收入水平与体育用品制造业发展的相互关系.结果表明:2003-2009年国民整体文化素质提升较快,人均收入水平增长势头良好,体育用品制造业整体发展状况较好;文化教育程度、国民收入水平与体育用品制造业发展均存在长期协整关系,我国绝大部分地区(区域)居民文化教育程度和人均收入水平都对体育用品制造业的发展产生长期正向的促进作用,全国及各地区国民收入水平对体育用品制造业发展的影响系数均高于文化教育程度,居民文化教育程度、人均收入水平对体育用品制造业发展的影响程度在不同地区(区域)间存在明显的差异性.最后就所得结论展开分析和讨论,并归纳出基本结论.     

Differential electronic detector to monitor apoptosis using dielectrophoresis-induced translation of flowing cells (dielectrophoresis cytometry)

The instrument described here is an all-electronic dielectrophoresis (DEP) cytometer sensitive to changes in polarizability of single cells. The important novel feature of this work is the differential electrode array that allows independent detection and actuation of single cells within a short section ( ～ 300?μm) of the microfluidic channel. DEP actuation modifies the altitude of the cells flowing between two altitude detection sites in proportion to cell polarizability; changes in altitude smaller than 0.25 μm can be detected electronically. Analysis of individual experimental signatures allows us to make a simple connection between the Clausius-Mossotti factor (CMF) and the amount of vertical cell deflection during actuation. This results in an all-electronic, label-free differential detector that monitors changes in physiological properties of the living cells and can be fully automated and miniaturized in order to be used in various online and offline probes and point-of-care medical applications. High sensitivity of the DEP cytometer facilitates observations of delicate changes in cell polarization that occur at the onset of apoptosis. We illustrate the application of this concept on a population of Chinese hamster ovary (CHO) cells that were followed in their rapid transition from a healthy viable to an early apoptotic state. DEP cytometer viability estimates closely match an Annexin V assay (an early apoptosis marker) on the same population of cells.     

社区教育经费投入不足的公共产品属性特征分析

社区教育是一种具有较强个人收益的竞争性、排他性准公共产品,强调国家和个人合理分担成本,因此可能在政府干预优先次序等级中排在比较后的位置,容易导致经费供应不足。教育部2015年社区教育工作情况调查统计数据也表明,社区教育经费没有列入经常性财政开支中,并且从最高值、平均值、中位值、最低值均远远低于普通教育、职业教育等教育形式,直接影响了社区教育政策的执行。     

学位类型与研究生资助——我国硕士研究生资助政策的瞄准效果

结合政策文本和大规模硕士研究生调查数据,从国家整体设计、院校实践和个人获得三个层面,分析我国研究生资助在不同学位类型硕士研究生中的配置问题。研究发现,当前国家层面的研究生资助政策关注资助体系的建立,不提倡对同学历、不同学位类型的研究生给予不同的资助待遇。但在院校实践中,学术型硕士和专业学位硕士面临不同的资助条件。虽然我国研究生资助的覆盖面逐渐广泛,资助规模逐渐增大,但是不同学位类型的研究生获得资助的情况存在着明显差异。学术硕士不仅学费低于专业硕士,而且其获得总资助、赠予型资助和报酬型资助的比例和金额都比专业硕士要高。