Fiber optic biosensor of immobilized firefly luciferase

Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. TheK _m′ for D-luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25°C respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10⁻⁹–10⁻⁵ mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.     

提高思想政治理论课教学实效性的思考

思想政治理论课教学是大学生思想政治教育的主渠道和主阵地。为了提高新形势下思想政治理论课教学的实效性，必须通过以下环节和措施来实现：重点提高思想政治理论课教师的政治素养，进一步优化教学内容，改进教学方法，完善实践和考试环节，使思想政治理论课教学在思想道德建设方面起到独特的作用。     

高等职业教育专业设置特色分析

专业设置是高等职业教育的基础。在教育比较的基础上，分析了高等职业教育专业设置的原则特色、结构特色、层次特色、名称的形式特色和机制特色，并就特色专业建设进行了探讨。力求为高等职业教育建设特色专业提供思路。     

试论李白的写景诗

李白写景诗的风格外观是多色调的,有神奇的美、壮丽的美,也3有清丽含蓄的美。李白写景诗的内涵是时代精神充分个性化的、形象的表现,盛唐气象通过李白的“济世”热情和飘逸个性,转化为空间时间化的动态意象。在艺术创造上,李白继承了“兴寄”和“自然”两大写景传统,在个性表现和自然生成的基础上,一方面尽量呈现对景物的原初感觉,另一方面又象征寄托着深厚的时代内容,是比兴兼得的典范。     

Introduction to this Special Issue

In 2001, for the first time in the history of the International Association for Counselling, International Conference was held in a developing Asian country–India, with the theme Families in Transition–Counselling in Transition–Meeting the Challenges Ahead. This special issue contains some of the presentations made by Indian participants during this conference which best reflect the challenges and diversities of counseling efforts with families in India. The first article was specially written for this issue in order to give an international relationship of this journal an in depth contextual understanding of Indian families in transition and the challenges they pose to the contemporary Indian counselor. Two articles are reflected to counseling families affected by socio-politial and economic changes. Five authors have written on counseling related to physical and mental disability/addiction and last two articles are reflected to authors experiences as family counselors.     

Kinetics study on biomass pyrolysis for fuel gas production

Kinetic knowledge is of great importance in achieving good control of the pyrolysis and gasification process and optimising system design. An overall kinetic pyrolysis scheme is therefore addressed here. The kinetic modelling incorporates the following basic steps: the degradation of the virgin biomass materials into primary products (tar, gas and semi-char), the decomposition of primary tar into secondary products and the continuous interaction between primary gas and char. The last step is disregarded completely by models in the literature. Analysis and comparison of predicted results from different kinetic schemes and experimental data on our fixed bed pyrolyser yielded very positive evidence to support our kinetic scheme. Project (No. 2001CB409600) Supported by the National Key Basic Research Program (973) of China     

天光云影共徘徊——申跃中创作命运思考(上)

申跃中几十年起伏多变的创作命运,始终没有离开过中国当代这一特定历史时期时代因素的缠绕。这一因素既把他涌向潮头,也把他推向低谷,使他与时代、与社会结下了永远的不解之缘。在这一过程中,作家对创作道路的自觉选择和对艺术境界的自觉追求对创作命运具有重要意义,时代感召和主体抉择的共同作用,使作家走上了一条成功之路。     

基于Windows2000环境下的缓冲区溢出

通过对Windows内存结构及进程空间分配情况的介绍,详细描述了Win2000环境下缓冲区溢出的原理,并提出了预防缓冲区溢出的有效方法。     

普通话二字组词语的动态声调

普通话二字组词语不同的声调组合方式 ,使前后位声调产生不同的动态变化形式。前位声调的动态变化明显表现在上声调变为低降调和中升调上 ;后位声调的动态变化则表现在调值的明显改变上。后位声调的动态变化更为普遍 ,应该引起足够的重视