高瞻远瞩

吸引了全世界眼球的党的十七大已经胜利闭幕,但胡总书记在报告中强调的:要"优先发展教育,建设人力资源强国。"使"现代国民教育体系更加完善,终身教育体系基本形成,全民受教育程度和创新人才培养水平明显提高。""发展远程教育和继续教育,建设全民学习、终身学习的学习型社会。""要努力使全体人民学有所教、劳有所得、病有所医、老有所养、住有所居。"至今仍回响在耳际,这是高瞻远瞩的盛世宏论。     

唐代音乐诗的表现艺术鉴赏

音乐是听觉艺术，无形无状，难于描写。唐代的音乐诗因音乐的繁荣而繁荣，诗人们在这个题材上表现出高超的技艺。本初步总结出四种表现手法，即“以声拟声”、“以形写声”、“侧面烘托”、“曲中有人”。     

Green Fluorescent Protein Recombinant Nisin as a Probe for Detection of Gram-Positive Bacteria

A great amount of foodborne pathogens were Gram-positive(G+) bacteria, a threat to public health. In this study, considering the binding ability of nisin towards G+ bacteria and the stable fluorescent ability of EGFP protein, a fluorescent nisin–EGFP protein probe was constructed by a gene engineering method. Nisin and EGFP were used as the receptor and fluorophore, respectively, to detect G+ bacteria. The nisin and egfp gene were amplified separately according to the sequence published in Gen Bank using unique primers. The two genes were cloned into a pET-28b(+) vector resulting in apET-28b(+)–nisin–egfp vector. The vector was transferred into Escherichia coli(E. coli) BL21(DE3) for expression. The expressed protein was extracted, purified by a Ni–NTA column, and then tested by the SDS-PAGE method to confirm its molecular weight. Listeria monocytogenes(L.monocytogenes), Staphylococcus aureus(S. aureus), and Micrococcus luteus(M. luteus) were used as the representations of G+ bacteria. E. coli O157, representing the gram-negative(G-) bacteria, was used as a negative control. The binding specificity of the recombinant protein was performed on two types of bacteria and then detected through fluorescent microscopy. The results indicated that the nisin–EGFP probe could detect G+ bacteria at 10~8CFU/mL.     

数字化学习资源的时空特点及作用机理

随着计算机和网络在教育教学中广泛深入地应用,数字化学习资源开始影响我们的学习和生活。研究者对于数字化学习资源投入了更多关注的目光,本文从数字化学习资源的内涵与特征出发,分析其时空特点和作用机理,以期对数字化学习资源的有效建设和应用提供指导。     

非线性Fredholm积分方程与分岔

本文研究了非线性Frcdholm积分方程的解数随参数k变化而发生分岔的规律:Ⅰ)当k〈0时,方程至少有一个负解;当0〈k〈k_1时,方程至少有一个正解。Ⅱ)当k〉k_n时,方程至少有(2n—1)个正解。并将这些结果应用到非线性Sturm—Iiouville问题。     

棒打鸳鸯原型及其成因探析

爱情悲剧在我国举不胜举。本文以爱情悲剧中的一种 (棒打鸳鸯 )作为论述对象 ,运用原型批评方法 ,将棒打鸳鸯原型概括为三个情节阶段 :男女相爱———棒从天降———劳燕纷飞 ,并探讨了其背后的真善美标准。     

用"四多四少"优化学生成长氛围

青少年学生的成长,需要老师的精心呵护。从育人的实践经验看,“四多四少”的做法,是我们常用的优化成长氛围的技巧之一。所谓“四多四少”,就是多鼓励,少否定;多谈心,少隔膜;多     

A Two-State Study of Family Child Care Engagement in Quality Rating and Improvement Systems: A Mixed-Methods Analysis

ABSTRACT

Research Findings. The current study is a mixed-methods investigation of family child care provider participation in voluntary Quality Rating and Improvement Systems (QRIS) in 2 states. Study 1 is an analysis of matched QRIS and child care licensing administrative data extracted from both states in May, 2014. Poverty and population density variables were added to the dataset by aligning U.S. Census data and Rural Urban Commuting Area codes (RUCA) to the matched state child care data. Participation patterns differed in the 2 states relative to provider characteristics (subsidy receipt, years licensed, and location). Study 2 consists of focus groups with participating (n = 22) and non-participating (n = 19) providers. Professionalism and financial incentives emerged as significant in attracting family child care providers to QRIS. Although family child care providers identified several clear benefits to QRIS, many more challenges emerged that negatively impact their participation. Practice or Policy. As most states are employing a QRIS framework to integrate quality improvement efforts, perspectives from this sector of early care and education are often overlooked. Study findings suggest that QRIS can be more responsive and effective with family child care providers if more consideration of their unique features are considered in systems design and implementation.     

对当前中小学教师继续教育课程设置问题的思考及建议

本文立足于思考当前中小学教师继续教育课程设置问题,主要论析:当前中小学教师继续教育课程设置的前提限定性、结构合理性及应遵循的原则等问题。     

对体育教育专业必修田径课程设计的探讨

以转变教育思想和观念为先导,以打破竞技体育为中心的课程设计方案为前提,建立一个以学校体育为中心,以培养合格体育师资为目标的,适用于体育教育专业需要的必修田径课程设计方案,推动田径运动课程建设和教学改革的深入发展,并配以教学方法的改革,落实必修田径课的课程目标和具体任务。