Agreement in activity energy expenditure assessed by accelerometer and self-report in adolescents: variation by sex, age, and weight status

The agreement between self-reported and objective estimates of activity energy expenditure was evaluated in adolescents by age, sex, and weight status. Altogether, 403 participants (217 females, 186 males) aged 13-16 years completed a 3-day physical activity diary and wore a GT1M accelerometer on the same days. Partial correlations (controlling for body mass) were used to determine associations between estimated activity energy expenditure (kcal · min(-1)) from the diary and accelerometry. Differences in the magnitude of the correlations were examined using Fisher's r to z transformations. Bland-Altman procedures were used to determine concordance between the self-reported and objective estimates. Partial correlations between assessments of activity energy expenditure (kcal · min(-1)) did not differ significantly by age (13-14 years: r = 0.41; 15-16 years: r = 0.42) or weight status (normal weight: r = 0.42; overweight: r = 0.39). The magnitude of the association was significantly affected by sex (Δr = 0.11; P < 0.05). The agreement was significantly higher in males than in females. The relationship between activity energy expenditure assessed by the objective method and the 3-day diary was moderate (controlling for weight, correlations ranged between 0.33 and 0.44). However, the 3-day diary revealed less agreement in specific group analyses; it markedly underestimated activity energy expenditure in overweight/obese and older adolescents. The assessment of activity energy expenditure is complex and may require a combination of methods.     

The force-time profile of elite front crawl swimmers

In this study, we used recently developed technology to determine the force-time profile of elite swimmers, which enabled coaches to make informed decisions on technique modifications. Eight elite male swimmers with a FINA (Federation Internationale de Natation) rank of 900+ completed five passive (streamline tow) and five net force (arms and leg swimming) trials. Three 50-Hz cameras were used to video each trial and were synchronized to the kinetic data output from a force-platform, upon which a motorized towing device was mounted. Passive and net force trials were completed at the participant's maximal front crawl swimming velocity. For the constant tow velocity, the net force profile was presented as a force-time graph, and the limitation of a constant velocity assumption was acknowledged. This allowed minimum and maximum net forces and arm symmetry to be identified. At a mean velocity of 1.92+0.06 m s?1, the mean passive drag for the swimmers was 80.3+4.0 N, and the mean net force was 262.4+33.4 N. The mean location in the stroke cycle for minimum and maximum net force production was at 45% (insweep phase) and 75% (upsweep phase) of the stroke, respectively. This force-time profile also identified any stroke asymmetry.     

Perceptions of change and certainty regarding the self-as-exerciser: a multistudy report

The purpose of these studies was to examine the relationship between perceptions of exercise-related changes (i.e., perceived mastery and physical change) and certainty with regard to the self-as-exerciser. It was hypothesized that seeing "change" would be associated with more favorable levels of exercise self-certainty and behavior relative to "no change." Online surveys were repeatedly administered across 4 months (Study 1) and 4 weeks (Study 2) to 196 university students (M(age) = 20.17), and 250 community dwellers (M(age) = 38.44), respectively. Data were analyzed via latent variable modeling procedures. Consistent with hypotheses, latent classes (i.e., subgroups) reflecting interindividual differences in levels and trajectories of perceived change were associated with distinct patterns of self-certainty and exercise behavior. The findings suggest that adults who experience mastery of skills and physiological changes also have greater self-certainty and exercise more regularly than those who do not see progress or feel as certain of their exercise identity.     

Perception and action in golf putting: skill differences reflect calibration

We assessed how golfers cope with the commonly observed systematic overshoot errors in the perception of the direction between the ball and the hole. Experiments 1 and 2, in which participants were required to rotate a pointer such that it pointed to the center of the hole, showed that errors in perceived direction (in degrees of deviation from the perfect aiming line) are destroyed when the head is constrained to move within a plane perpendicular to the green. Experiment 3 compared the errors in perceived direction and putting errors of novice and skilled players. Unlike the perceived direction, putting accuracy (in degrees of deviation from the perfect aiming line) was not affected by head position. Novices did show a rightward putting error, while skilled players did not. We argue that the skill-related differences in putting accuracy reflect a process of recalibration. Implications for aiming in golf are discussed.     

Separation and enrichment of sodium-motile bacteria using cost-effective microfluidics

Many motile bacteria are propelled by the rotation of flagellar filaments. This rotation is driven by a membrane protein known as the stator-complex, which drives the rotor of the bacterial flagellar motor. Torque generation is powered in most cases by proton transit through membrane protein complexes known as stators, with the next most common ionic power source being sodium. Sodium-powered stators can be studied through the use of synthetic chimeric stators that combine parts of sodium- and proton-powered stator proteins. The most well studied example is the use of the sodium-powered PomA-PotB chimeric stator unit in the naturally proton-powered Escherichia coli. Here we designed a fluidics system at low cost for rapid prototyping to separate motile and non-motile populations of bacteria while varying the ionic composition of the media and thus the sodium-motive force available to drive this chimeric flagellar motor. We measured separation efficiencies at varying ionic concentrations and confirmed using fluorescence that our device delivered eightfold enrichment of the motile proportion of a mixed population. Furthermore, our results showed that we could select bacteria from reservoirs where sodium was not initially present. Overall, this technique can be used to implement the selection of highly motile fractions from mixed liquid cultures, with applications in directed evolution to investigate the adaptation of motility in bacterial ecosystems.     

Bandpass sorting of heterogeneous cells using a single surface acoustic wave transducer pair

Separation and sorting of biological entities (viruses, bacteria, and cells) is a critical step in any microfluidic lab-on-a-chip device. Acoustofluidics platforms have demonstrated their ability to use physical characteristics of cells to perform label-free separation. Bandpass-type sorting methods of medium-sized entities from a mixture have been presented using acoustic techniques; however, they require multiple transducers, lack support for various target populations, can be sensitive to flow variations, or have not been verified for continuous flow sorting of biological cells. To our knowledge, this paper presents the first acoustic bandpass method that overcomes all these limitations and presents an inherently reconfigurable technique with a single transducer pair for stable continuous flow sorting of blood cells. The sorting method is first demonstrated for polystyrene particles of sizes 6, 10, and 14.5 μm in diameter with measured purity and efficiency coefficients above 75 ± 6% and 85 ± 9%, respectively. The sorting strategy was further validated in the separation of red blood cells from white blood cells and 1 μm polystyrene particles with 78 ± 8% efficiency and 74 ± 6% purity, respectively, at a flow rate of at least 1 μl/min, enabling to process finger prick blood samples within minutes.     

A Systematic Review of Sequential Multiple-Assignment Randomized Trials in Educational Research

Educational Psychology Review - The purpose of this systematic review is to describe the state of the art of sequential multiple-assignment randomized trials in education research. An iterative,...     

An emerging community in online mathematics teacher professional development: an interactional perspective

Online collaborative and content-focused professional development (PD) is becoming an increasingly important setting for supporting mathematics teachers’ professional learning. The purpose of this study was to better understand the process by which a community emerges in such a PD setting by examining how the cohesiveness of 21 mathematics teachers’ social network evolves and associated shifts in the quality of mathematics teachers’ mathematical discourse. We employed social network analysis (SNA) to examine the evolving cohesiveness of mathematics teachers’ social network and coding procedures to examine teachers’ mathematical discourse. A key finding was the documentation of an emergent divide between participation in the core and periphery during initial weeks of the PD and then a reduced divide and emergence of a social network that resembles a community. We argue that the instructor’s pattern of participation that included distributing their interactions across the subgroups while sending a common message regarding expectations for mathematical discourse in the PD may have contributed to the community formation process. We propose the Interaction Assessment Model, which outlines an approach for PD facilitators to use SNA as a feedback mechanism to differentiate facilitation of online collaborative and content-focused PD and build online communities.