Tunable spatial heterogeneity in structure and composition within aqueous microfluidic droplets

In this paper, we demonstrate biphasic microfluidic droplets with broadly tunable internal structures, from simple near-equilibrium drop-in-drop morphologies to complex yet uniform non-equilibrium steady-state structures. The droplets contain an aqueous mixture of poly(ethylene glycol) (PEG) and dextran and are dispensed into an immiscible oil in a microfluidic T-junction device. Above a certain well-defined threshold droplet speed, the inner dextran-rich phase is "stirred" within the outer PEG-rich phase. The stirred polymer mixture is observed to exhibit a near continuum of speed and composition-dependent phase morphologies. There is increasing interest in the use of such aqueous two-phase systems in microfluidic devices for biomolecular applications in a variety of contexts. Our work presents a method to go beyond equilibrium phase morphologies in generating microfluidic "multiple" emulsions and at the same time raises the possibility of biochemical experimentation in benign yet complex biomimetic milieus.     

A functional agility short-term fatigue protocol changes lower extremity mechanics

The purpose of this study was to evaluate the effects of a functional agility fatigue protocol on lower extremity biomechanics between two unanticipated tasks (stop-jump and sidestep). The subjects consisted of fifteen female collegiate soccer athletes (19±0.7 years, 1.67±0.1 m, 61.7±8 kg) free of lower extremity injury. Participants performed five trials of stop-jump and sidestep tasks. A functional short-term agility protocol was performed, and immediately following participants repeated the unanticipated running tasks. Lower extremity kinematic and kinetic values were obtained pre and post fatigue. Repeated measures analyses of variance were conducted for each dependent variable with an alpha level set at 0.05. Knee position post-fatigue had increased knee internal rotation (11.4±7.5° vs. 7.9±6.5° p=0.011) than pre-fatigue, and a decreased knee flexion angle (-36.6±6.2° vs. ?40.0±6.3°, p = 0.003), as well as hip position post-fatigue had decreased hip flexion angle (35.5±8.7° vs. 43.2±9.5°, p = 0.002). A quick functional fatigue protocol altered lower extremity mechanics of Division I collegiate soccer athletes during landing tasks. Proper mechanics should be emphasized from the beginning of practice/game to aid in potentially minimizing the effects of fatigue in lower extremity mechanics.     

BMI group-related differences in physical fitness and physical activity in preschool-age children: a cross-sectional analysis

In the Ballabeina study, we investigated age- and BMI-group-related differences in aerobic fitness (20 m shuttle run), agility (obstacle course), dynamic (balance beam) and static balance (balance platform), and physical activity (PA, accelerometers) in 613 children (M age = 5.1 years, SD = 0.6). Normal weight (NW) children performed better than overweight (OW) children in aerobic fitness, agility, and dynamic balance (all p <.001), while OWchildren had a better static balance (p < .001). BMI-group-related differences in aerobic fitness and agility were larger in older children (p for interaction with age = .01) in favor of the NW children. PA did not differ between NW and OW (p > or = .1), but did differ between NW and obese children (p < .05). BMI-group-related differences in physical fitness can already be present in preschool-age children.     

Relationship between serum interleukin-1beta levels and acute phase response proteins in patients with familial Mediterranean fever

Introduction:

The aim of this study was to investigate whether serum levels of interleukin-1β (IL–1β) has any possible correlation on inflammatory parameters such as C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and fibrinogen concentration in patients with familial Mediterranean fever (FMF) patients during attack-free period.

Materials and methods:

The serum levels of IL-1β, as an indicator of cytokines status, and the acute phase response proteins, CRP, ESR and fibrinogen levels were evaluated in 35 attack-free patients with FMF and 25 healthy volunteers.

Results:

Serum IL-1β levels were significantly higher in patients with FMF than control subjects (P = 0.018). There was no statistically significant difference in the serum levels of ESR, CRP and fibrinogen between two groups (P = 0.181, P = 0.816, P = 0.686, respectively). There was a significant correlation between IL-1β and CRP (r = 0.513, P = 0.002) values of FMF group.

Conclusions:

In conclusion, our results confirm the presence of increased IL-1β levels in FMF patients during attack-free period. Serum IL-1β values seems to correlate with CRP levels. The elevation of IL-1β levels may be important in monitoring subclinical inflammation of attack free period in FMF patients.     

The sciences of subjectivity

In historical and ethnographic studies of the making of scientific knowledge, there has been a long-standing fascination with deflating certain stories about objectivity. Among the resources used to achieve that deflation have been the notions of subjectivity, which has been treated more as a trouble for objectivity than as a knowledge-making mode open to systematic study. I describe notions of subjectivity implicated in that inattention; I trace potentially constructive links between contemporary science studies and resources in 18th-century philosophical aesthetics; I draw notice to available engagements with the mode of subjectivity known as taste, and, especially, gustation and olfaction; and I suggest ways in which we might study the achievement of intersubjectivity in these domains.     

When humans are the exception: cross-species databases at the interface of biological and clinical research

Cross-species comparison has long been regarded as a stepping-stone for medical research, enabling the discovery and testing of prospective treatments before they undergo clinical trial on humans. Post-genomic medicine has made cross-species comparison crucial in another respect: the 'community databases' developed to collect and disseminate data on model organisms are now often used as a template for the dissemination of data on humans and as a tool for comparing results of medical significance across the human-animal boundary. This paper identifies and discusses four key problems encountered by database curators when integrating human and non-human data within the same database: (1) picking criteria for what counts as reliable evidence, (2) selecting metadata, (3) standardising and describing research materials and (4) choosing nomenclature to classify data. An analysis of these hurdles reveals epistemic disagreement and controversies underlying cross-species comparisons, which in turn highlight important differences in the experimental cultures of biologists and clinicians trying to make sense of these data. By considering database development through the eyes of curators, this study casts new light on the complex conjunctions of biological and clinical practice, model organisms and human subjects, and material and virtual sources of evidence--thus emphasizing the fragmented, localized and inherently translational nature of biomedicine.     

From 'following the push of nature' to 'restoring one's proper sex'--cortisone and sex at Johns Hopkins's Pediatric Endocrinology Clinic

In 1955, a paradigm shift in the conception of sex is said to have taken place, when psychologist John Money at Johns Hopkins's Pediatric Endocrinology Clinic argued that 'hermaphroditic' children could be assigned a sex contradictory to their biological sex. Rather than being born male or female, he claimed, these children learned to be boys or girls. Money was subsequently credited the invention of the term gender role. However, Money only confirmed a practice that was established at the clinic several years before his intervention. The clinic's director Lawson Wilkins (1894-1963) had already recommended that certain children, virilized by congenital adrenal hyperplasia, should be raised in the male sex, even though they were by all medical standards of the time female. What mattered for him was assigning the sex that seemed 'better' for these children. What constituted the 'better sex' was contingent on the child's psyche and habitus, social expectations, and on the range of medical and surgical interventions available at the time.     

科学与搜寻罪犯

现代科学在许多方面应用于解决犯罪问题。其范围包括血迹的实验室检验, 指纹的分类与搜寻,以及犯罪调查系统的计算机坐阵指挥。作者为科学在这个有趣领域的应用提供了某些例证。     

微营养素和肉品质量的关系

日粮中微营养素硒(Se)能够引起肌肉组织的生理变化,从而改变畜禽肉品的品质.本文着重阐明硒和VE在细胞膜和细胞质的抗氧化中独特应用,进而对肉品的形成和贮存过程中保持肉品的完整性起明显作用.