集装箱桥吊前大梁主铰冲击特征分析技术的研究

应用小波包分析技术,结合Hilbert包络技术,提出了小波包能量包络技术.该技术能够很好地表征桥吊在工作时产生的振动冲击等非平稳信号的状态特征,为桥吊的状态监测和安全评估提供了一种抗噪能力强、特征表述灵敏的特征抽取技术,并在已开发的状态监测和评估系统中得到了实践,取得了很好的应用.     

Herceptin functionalized microfluidic polydimethylsiloxane devices for the capture of human epidermal growth factor receptor 2 positive circulating breast cancer cells

Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (～80% capture yield) HER2 positive cells from full blood.     

Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry

A method for monitoring the biological exocytotic phenomena on a microfluidic system was proposed. A microfluidic device coupled with functionalities of fluorescence imaging and amperometric detection has been developed to enable the real-time monitoring of the exocytotic events. Exocytotic release of single SH-SY5Y neuroblastoma cells was studied. By staining the cells located on integrated microelectrodes with naphthalene-2,3-dicarboxaldehyde, punctuate fluorescence consistent with localization of neurotransmitters stored in vesicles was obtained. The stimulated exocytotic release was successfully observed at the surface of SH-SY5Y cells without refitting the commercial inverted fluorescence microscope. Spatially and temporally resolved exocytotic events from single cells on a microfluidic device were visualized in real time using fluorescence microscopy and were amperometrically recorded by the electrochemical system simultaneously. This coupled technique is simple and is hoped to provide new insights into the mechanisms responsible for the kinetics of exocytosis.     

买方市场势力、市场竞争环境与研发投入——基于高新技术上市公司的经验证据

  客户作为企业重要的利益相关者,对企业行为具有广泛而深刻的影响。买方市场势力是客户的重要特征之一,对企业的创新决策发挥着不可忽视的作用。本文以2012—2016年沪深两市A股中的610家高新技术企业上市公司为样本,从市场竞争强度和市场动态性两个角度实证检验了不同市场竞争环境下买方市场势力对企业创新研发投入的影响。结果表明：市场竞争程度越激烈,买方市场势力对创新研发投入产生的“促进激励效应”越明显;企业所处行业的市场动态性越高,买方市场势力对创新研发投入产生的“促进激励效应”越显著。     

城市群规划引导下的政府合作与区域创新能力

本文以11个城市群规划的颁布作为一项准自然实验,采用多期双重差分法,从创新视角考察城市群的经济效应。结果显示：（1）城市群规划的实施对城市群的区域创新活动具有正向推动作用。（2）原先集群程度和一体化程度越高、内部技术水平越接近的城市群收益越大;相比较大城市和中心城市,非中心城市和规模越小的城市在城市群规划实施后创新活动的增长更明显。（3）这其中的作用机制在于城市群规划能够引导地方政府积极合作,打破物理边界,寻求区域创新合作,促进内部一体化程度加深。     

不同负荷跑台训练对大鼠大脑海马形态结构及VEGF、ET、eNOS表达的影响

目的探讨不同负荷跑台训练对大鼠大脑皮质海马区形态结构及VEGF、eNOS表达的影响.方法将45只大鼠随机分为正常对照组、一般负荷训练组和过度负荷训练组,每组15只,训练8周后,对海马组织进行形态学观察并进行VEGF水平测定以及内皮型一氧化氮合酶(eNOS)灰度值扫描.结果(1)过度负荷训练组大鼠大脑海马组织中锥体细胞数目较正常对照组与一般负荷训练组有明显的下降(P<0.01);(2)过度负荷训练组大鼠大脑海马区组织形态出现病理现象;(3)运动组ET、VEGF含量较对照组显著性增高(P<0.01),过度负荷训练组ET、VEGF含量较一般负荷训练组有明显增高(P<0.05);(4)运动组eNOS表达较对照组明显增强(JP<0.01),而一般负荷训练组与过度负荷训练组eNOS表达无明显差异.结论适宜的运动对海马组织信息的传递、学习、记忆过程以及调节脑血流量,提高脑血管膜自身保护都产生积极、良好的影响,而过度负荷的运动使脑组织缺血缺氧,导致海马组织中细胞的损伤.     

传统武德的当代教育价值对塑造大学生健康人格的积极作用

传统武德作为传统的道德规范具有良好的教育功能,其基本内容＂尊师重道,精忠报国;讲礼守信,重义轻利;立身正直,谦和仁爱;自强不息,厚德载物＂可以从多方面影响并塑造大学生的健康人格。     

试探中国武术的功能与价值

中国武术发展到今天已经不仅仅是作为一种社会形态而存在,它更是作为一种文化、一种信仰深入国人的生活世界,成为国人心中不可或缺的、血脉相连的一部分。它在经济上与生产劳动密切相关,在政治上与维护社会稳定密切相关,在军事上与强大国防不可分割,在文化上更是不仅丰富了人民生活而且具有极大的教育和学术价值。文章通过对中国武术在经济、政治、文化、军事等方面价值和功能的揭示能为武术未来的发展方向提供一点思路,并使更多的人能够更好地了解、应用和感受武术。     

原地双手后抛实心球的技术分析与训练方法研究——关于体育高考术科考试项目分析

原地双手后抛实心球是广西体育高考术科考试中必考的一个项目,文章通过对原地双手后抛实心球技术进行详细分析,并针对它的动作结构进行系统的科学训练,以期为提高考生考试成绩和广大体育教师的业余训练提供可靠依据。     

羽毛球运动员灵敏素质训练的内容和方法

随着现代羽毛球运动训练手段的日趋科学化,在提高专项技术水平的同时,人们已经开始高度重视发展羽毛球运动员的各项素质,特别是灵敏素质。文章运用文献资料法和专家访谈法,根据羽毛球运动的特点和生物学原理指出灵敏训练对羽毛球运动训练的重要性,并且从不同的角度对羽毛球运动员运动训练中的灵敏素质的训练内容和方法进行了阐述,以期为专业羽毛球运动员身体灵敏素质训练提供参考,增进其羽毛球运动水平。