bmo-miR-0001 and bmo-miR-0015 down-regulate expression of <Emphasis Type="Italic">Bombyx mori</Emphasis> fibroin light chain gene in vitro

Based on bioinformatic analysis, we selected two novel microRNAs (miRNAs), bmo-miR-0001 and bmomiR-0015, from high-throughput sequencing of the Bombyx mori larval posterior silk gland (PSG). Firstly, we examined the expression of bmo-miR-0001 and bmo-miR-0015 in 12 different tissues of the 5th instar Day-3 larvae of the silkworm. The results showed that the expression levels of both bmo-miR-0001 and bmo-miR-0015 were obviously higher in the PSG than in other tissues, implying there is a spatio-temporal condition for bmo-miR-0001 and bmo-miR-0015 to regulate the expression of BmFib-L. To test this hypothesis, we constructed pri-bmo-miR-0001 expressing the plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-0001-SV40] and pri-bmo-miR-0015 expressing the plasmid pcDNA3.0 [ie1-egfp-pribmo-miR-0015-SV40]. Finally, the BmN cells were harvested and luciferase activity was detected. The results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected by pcDNA3.0 [ie1-egfp-pri-bmomiR-0001-SV40] or pcDNA3.0 [ie1-egfp-pri-bmo-miR-0015-SV40] with pGL3.0 [A3-luc-Fib-L-3′UTR-SV40], suggesting that both bmo-miR-0001 and bmo-miR-0015 can down-regulate the expression of BmFib-L in vitro.     

新时期班主任工作初探

新时期,尤其是提倡素质教育以来,教育理念,教学方式和育人方法发生了深刻的变化,班主任只有转变观念和方法,不断探索才能适应时代,否则将被淘汰.     

应用型大学培养高技能人才课程模式的反思与构建--以天津工程师范学院"本科+技师"培养模式为例

针对现有高技能人才课程模式在课程类、形、质的结构上存在的弊端,天津工程师范学院机械系机械制造及自动化专业以“技术 人文”为理念,“本科 技师”为目标,构建应用型大学培养高技能人才的“模块 系列”模式,其中“模块”包括通识教育课模块、专识教育课模块和特识教育模块“;系列”包括必修课和选修课系列。     

高分子网络法制备In2O3超细粉的工艺研究

以硝酸铟为起始原料, 利用高分子网络法合成纯的单相In2O3纳米晶. 采用X射线粉末衍射(XRD)和透射电镜(TEM)等检测手段对所得粉体的组成、大小和微观形貌进行了表征和分析. 同时, 根据XRD衍射图谱结果进一步讨论了不同原料质量浓度、煅烧温度和煅烧时间对产物粒径的影响. 实验结果表明所得晶粒晶相单一, 粒度均匀并且可以选择适宜的条件制备所需粒径的纳米氧化物.     

基于课程群的电子信息工程创新实验体系改革探索

针对现阶段高等工程专业实践教学环节普遍存在的不足,旨在全面深化实验教学体系改革,提出并构建了一种基于课程群的电子信息工程层次化实验体系,详细地描述了新体系的构建思想、实施方案及其构建过程中需要注意的主要问题。新体系在学生专业实践能力与工程创新意识培养方面均取得了显著成效,在全校范围内起到了较好的示范作用,并为其他高校在同类实践教学改革方面提供了新的思路。     

以创新能力培养为导向的机械类专业实践课程改革研究

文章根据现代工程教育理念,分析了当前机械类专业教学中存在的主要问题及形成的原因,提出了以创新能力培养为导向的机械类专业实践课程改革与研究的思路、总体目标和改革的主要内容,以组织学生参加全国大学生机械创新设计大赛为例总结了已取得的经验。     

Maximizing modern distribution of complex anatomical spatial information: 3D reconstruction and rapid prototype production of anatomical corrosion casts of human specimens

Anatomical corrosion casts of human specimens are useful teaching aids. However, their use is limited due to ethical dilemmas associated with their production, their lack of perfect reproducibility, and their consumption of original specimens in the process of casting. In this study, new approaches with modern distribution of complex anatomical spatial information were explored to overcome these limitations through the digitalization of anatomical casts of human specimens through three‐dimensional (3D) reconstruction, rapid prototype production, and Web‐based 3D atlas construction. The corrosion cast of a lung, along with its associated arteries, veins, trachea, and bronchial tree was CT‐scanned, and the data was then processed by Mimics software. Data from the lung casts were then reconstructed into 3D models using a hybrid method, utilizing both “image threshold” and “region growing.” The fine structures of the bronchial tree, arterial, and venous network of the lung were clearly displayed and demonstrated their distinct relationships. The multiple divisions of bronchi and bronchopulmonary segments were identified. The 3D models were then uploaded into a rapid prototype 3D printer to physically duplicate the cast. The physically duplicated model of the lung was rescanned by CT and reconstructed to detect its production accuracy. Gross observation and accuracy detection were used to evaluate the duplication and few differences were found. Finally, Virtual Reality Modeling Language (VRML) was used to edit the 3D casting models to construct a Web‐based 3D atlas accessible through Internet Explorer with 3D display and annotation functions. Anat Sci Educ. © 2012 American Association of Anatomists.     

面向创新能力培养的服装设计与工程专业实验教学体系研究与实践

在分析专业人才培养方案和服装产业现状的基础上,从重组实验教学内容、改革实验教学模式、改变实验教学手段和完善实验考核方式等4个方面入手,构建了与创新能力培养目标相适应的多层次、模块化服装设计与工程专业实验教学新体系,提高了实验教学的效率和学习效果,培养了学生的动手能力、产业适应能力和创新、创业能力。     

Forecasting performance of grey prediction for education expenditure and school enrollment

GM(1,1) and GM(1,1) rolling models derived from grey system theory were estimated using time-series data from projection studies by National Center for Education Statistics (NCES). An out-of-sample forecasting competition between the two grey prediction models and exponential smoothing used by NCES was conducted for education expenditure and school enrollment under the assumption that grey prediction was as promising as NCES's forecasting technique in dealing with univariate time-series data while some other determinants of the variables under examination were excluded. The purpose of this study, therefore, was to verify that the GM(1,1), and GM(1,1) rolling models would provide forecasts that were at least as accurate as the NCES's approach to extrapolating education expenditure and school enrollment. The findings revealed that the forecasting efficiency of GM(1,1) rolling model was superior to exponential smoothing and GM(1,1) model. The results can offer valuable insights and provide a basis for further research in model building for short-term estimation on educational statistics.     

《看不见的人》中的布鲁斯哲学

布鲁斯是美国黑人民族文化的象征,也是黑人文学文本研究的原始依据和文化源泉。拉尔夫·埃利森的著作《看不见的人》以其独特的风格和艺术魅力成为当代美国小说的经典之作。埃里森创造性地将黑人布鲁斯音乐融会贯通于整部作品之中,以布鲁斯精髓深刻地阐释出黑人寻求自我身份及其文化身份的小说主题。本文介绍了布鲁斯所包含的三个相对独立又彼此相关的概念,并着重分析了布鲁斯哲学在小说中的体现,指出布鲁斯不仅是美国黑人苦难的生动诉说,更是其坚韧不屈、勇往直前的力量之源。