Tandem Biocatalysis by CotA-TJ102@UIO-66-NH2 and Novozym 435 for Highly Selective Transformation of HMF into FDCA

2,5-Furandicarboxylic acid(FDCA) is a potential biorenewable chemical for applications including plastics, polyamides, drugs, etc. The selective biosynthesis of FDCA from 5-hydroxymethylfurfural(HMF) by a specific enzyme poses a great challenge. In this study, we reported an efficient strategy to produce FDCA from HMF by the tandem biocatalysis of laccase(Cot A-TJ102@UIO-66-NH_2) and Novozym 435. For the first step, a nanoparticle metal–organic framework was synthesized as a carrier to immobilize Cot A-TJ102@UIO-66-NH_2, which was assigned for the production of 5-formyl-2-furancarboxylic acid(FFCA) and featured an enzyme loading of 255.54 mg/g, specific activity of 135.90 U/mg, and solid loading ratio of 99.65%. Under optimal conditions, an ideal FFCA yield of 98.5% was achieved, and the Cot A-TJ102@UIO-66-NH_2 presented a high recycling capacity after 10 cycles. For the second step, Novozym 435 was applied for the further conversion of FFCA into FDCA, presenting a high FDCA yield of 95.5% under the optimized conditions. Novozym 435 also exhibited a high recyclability after eight cycles. As a result, the tandem biocatalysis strategy provided a 94.2% FDCA yield from HMF, indicating its excellence as a method for FDCA production.     

职业教育发展的市场调节与政府干预

近来,有关政府与市场的关系问题一直是国家发展领域颇有争议的话题.本文通过分析市场经济条件下职业教育市场机制和政府干预各自的局限性,探讨新形势下我国职业教育发展的动力机制.     

太原市12319城建服务热线系统的设计构想

高科技助力“为民热线”随着科技的快速发展,城市便民电话服务系统运用现代通信、网络技术和计算机技术,建立起了综合的热线服务平台及调度管理网络。     

析我国合同法中的缔约过失责任

缔约上的过失责任制度,已为世界大多数发达国家法律所确认,我国合同法对此出作了相应规定.该规定填补了我国民法中对缔约过失责任无明文完整规定的空白,对于保护缔约阶段遭受损失的一方当事人的利益,完善债法理论体系,具有极其重要的意义.     

论建立与社会主义市场经济相适应的道德价值观

市场经济遵循等价交换、自由竞争、利润最大化等原则 ,但社会主义市场经济在肯定个人利益的同时 ,必须加强集体主义的价值导向     

自由教育的历史演变及当代特征

始于古希腊时期的自由教育思想，直到今天仍然在教育理论和实践领域发挥着影响力，考察自由教育的历史演变及当代特征，有助于准确地理解和把握自由教育思想。     

校园网建设要注重前期的规划与设计

提出在校园网建设前期应明确其使用对象及功能；讨论了其规划与设计中在基础建设、软件建设、队伍建设及依据学校自身特点等方面应注重的几个主要问题。     

非英语专业研究生跨文化交际能力的培养

综述了国内外学者对跨文化交际能力研究的现状,构建了培养研究生跨文化交际能力的教学模式,提出了培养方法,目的是提高研究生的跨文化交际能力。     

浅析财务会计和管理会计的关系

会计是一个信息系统,近代的会计,一般是以企业会计作为主体,财务会计和管理会计是企业会计的两个重要领域。二者相辅相成,相互补充,既有联系,又有区别。只有正确认识财务会计和管理会计的区别和联系,才能充分发挥各自在企业管理中应有的职能。本文就这一问题进行了初步的探讨。     

Can Pragmatic Competence Be Taught in an EFL Classroom?

An L2 learner’s pragmatic competence is an inseparable part of his communicative competence.Large quantities of aspects of pragmatic competence are teachable.Classroom is the place where an L2 learner’s pragmatic competence can be developed.Though classroom instruction has some negative effect,efforts could be done to arrange relevant awareness-raising and communication-practice activities.