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991.
本文介绍了河北钢铁集团唐钢热轧1700mm生产线的在线行走式板型仪的测试原理、设备构成、主要技术性能、应用范围.整套系统结构简单,成本低廉,抗干扰性好,调试方便. 相似文献
992.
在理论上将知识产权战略实施与区域经济社会发展诸项战略相结合,明确了"夯实竞争基础、增强竞争潜力、强化竞争实力"目标定位,而且在应用上更具操作性和测度性,可凸显区域振兴知识产权事业的显示度,以利于区域科技优势向知识产权优势,再向区域经济可持续发展优势的转变。 相似文献
993.
994.
A method for monitoring the biological exocytotic phenomena on a microfluidic system was proposed. A microfluidic device coupled with functionalities of fluorescence imaging and amperometric detection has been developed to enable the real-time monitoring of the exocytotic events. Exocytotic release of single SH-SY5Y neuroblastoma cells was studied. By staining the cells located on integrated microelectrodes with naphthalene-2,3-dicarboxaldehyde, punctuate fluorescence consistent with localization of neurotransmitters stored in vesicles was obtained. The stimulated exocytotic release was successfully observed at the surface of SH-SY5Y cells without refitting the commercial inverted fluorescence microscope. Spatially and temporally resolved exocytotic events from single cells on a microfluidic device were visualized in real time using fluorescence microscopy and were amperometrically recorded by the electrochemical system simultaneously. This coupled technique is simple and is hoped to provide new insights into the mechanisms responsible for the kinetics of exocytosis. 相似文献
995.
Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (~80% capture yield) HER2 positive cells from full blood. 相似文献
996.
We demonstrated a simple method for the device design of a staggered herringbone micromixer (SHM) using numerical simulation. By correlating the simulated concentrations with channel length, we obtained a series of concentration versus channel length profiles, and used mixing completion length Lm as the only parameter to evaluate the performance of device structure on mixing. Fluorescence quenching experiments were subsequently conducted to verify the optimized SHM structure for a specific application. Good agreement was found between the optimization and the experimental data. Since Lm is straightforward, easily defined and calculated parameter for characterization of mixing performance, this method for designing micromixers is simple and effective for practical applications. 相似文献
997.
传统武德作为传统的道德规范具有良好的教育功能,其基本内容"尊师重道,精忠报国;讲礼守信,重义轻利;立身正直,谦和仁爱;自强不息,厚德载物"可以从多方面影响并塑造大学生的健康人格。 相似文献
998.
中国武术发展到今天已经不仅仅是作为一种社会形态而存在,它更是作为一种文化、一种信仰深入国人的生活世界,成为国人心中不可或缺的、血脉相连的一部分。它在经济上与生产劳动密切相关,在政治上与维护社会稳定密切相关,在军事上与强大国防不可分割,在文化上更是不仅丰富了人民生活而且具有极大的教育和学术价值。文章通过对中国武术在经济、政治、文化、军事等方面价值和功能的揭示能为武术未来的发展方向提供一点思路,并使更多的人能够更好地了解、应用和感受武术。 相似文献
999.
1000.
随着现代羽毛球运动训练手段的日趋科学化,在提高专项技术水平的同时,人们已经开始高度重视发展羽毛球运动员的各项素质,特别是灵敏素质。文章运用文献资料法和专家访谈法,根据羽毛球运动的特点和生物学原理指出灵敏训练对羽毛球运动训练的重要性,并且从不同的角度对羽毛球运动员运动训练中的灵敏素质的训练内容和方法进行了阐述,以期为专业羽毛球运动员身体灵敏素质训练提供参考,增进其羽毛球运动水平。 相似文献