澳大利亚职业教育迅速发展的原因探析

20世纪70年代以来，澳大利亚职业教育迅速发展的主要原因是：重视职业教育，建立完整发达的职业教育体系，加大职业教育改革的力度，采取多种措施，加强政府统筹和宏观调控；确立能力本位的职业教育思想，引入竞争机制，建立市场化机制，采取形式多样的灵活的办学体制，不断增加职业教育投资，多渠道筹措职业教育经费，重视职业教育师资队伍建设。     

湖州非物质文化遗产与乡村旅游融合发展研究

非物质文化遗产需要传承和弘扬,地方旅游业发展需要文化支撑,如何将二者融合发展,值得研究。本文收集、整理湖州非物质文化遗产资料,对其数量、类型、分布等进行分析,并实地调查了湖州旅游发展过程中对非物质文化遗产的利用现状,提出湖州乡村旅游应充分利用非物质文化遗产,可从打造舞台盛宴、创新导游解说、打造非遗属性旅游纪念品三个方面提升乡村旅游的文化内涵。这样,既可以更好地保护地方非物质文化遗产,又可以推动地方旅游文化事业,二者的结合是双赢之举。     

Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.     

培养学生质疑问难能力策略研究

小学阶段是培养学生质疑问难能力的基础和关键。培养学生质疑问难能力是素质教育的需要，有助于学生良好个性的形成。培养学生质疑问难能力，要研究学生学习心理、学习环境和自身的知识和能力，同时也要教给学生质疑问难的方法。     

高职院校教学管理工作的探索

在新世纪新形势高职院校教学管理工作有新的要求; 目前高职院校在教学管理中存在的问题; 加强高职院校教学管理工作的建议和措施。     

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.     

面向大规模定制的产品族设计

在大规模定制生产模式下,基于产品平台的产品族开发战略是企业在竞争中确定胜利的关键。在产品平台上建立的产品族结构能够尽可能多的满足不同市场单元的要求,同时保证了产品设计的相对稳定性。本文介绍了产品族和产品平台的概念,以及基于产品平台的产品族开发战略。     

新时代高校思政课教师继续教育现状及对策研究

2018年《关于全面深化新时代教师队伍建设改革的意见》对教师继续教育提出了新要求,也为思政课教师的继续教育指明新方向和创新的新源泉.因此,文章在基于高校思政课教师继续教育现状分析的基础上,依据文件精神,紧紧把握政治方向,将文件精神融入继续教育全过程中,提出立德树人,强化继续教育德育功能;融合情景教育教学,构建"逆序创新"教育模式等对策,以解决新时代高校思政课教师继续教育所存在的问题.     

对"数学教师专业化问题"的几点思考

数学教师专业化是当前数学教育研究的热点问题。专业化数学教师要做到师范性与学术性相统一，应具备数学、教育心理学等修养。专业化数学教师要注重教育科研，重视初等数学研究，注重自身能力结构的改善。开展实验研究是培养专业化数学教师的有效途径。     

审美与创新

新的世纪,经济全球化,科技创新国际化,已成了知识经济时代的总体趋势。创新即生存、即发展,是支撑一个国家、一个民族的脊梁和灵魂。江泽民总书记强调指出:“创新是一个民族进步的灵魂,是一个国家兴旺发达的不竭动力。”很多专家学者或从经济发展、社会进步的角度,或从文化知识的角度,或从科技发展的角度,或从人类创造实践的角度,从理性上对创新进行了有益的研究和探讨,其中不乏真知灼见。但从审美的角度来对创新进行研究和探讨,却鲜有人为。本文拟从审美的角度,就创新谈自己的一管之见。