Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

理学人格对宋代山水画审美理想的影响

宋代理学家们所倡导的理想人格不仅仅是社会伦理的内涵,它包含着宋人对人生形而上的思考。虽然山水画学中的“理”和理学的“理”含义不完全相同,但它无疑是理学这个时代强音中的一个音符。画家笔下的层峦叠嶂、烟柳渔浦、寒林远水表达的是创作主体对世界与人生的思考,以有形之象呈现内在之理,画家在“理趣”的玩味中得到性情的陶染和灵魂的提升。宋代山水精、妙、神、逸诸品众多当与其兼备理、气、趣有着直接关系。而理、气、趣又来自创作主体所受到的理学人格的薰陶。宋代山水画取得的辉煌成就,不能无视宋代理学的积极影响。     

大学课程设计自学指南:如何设计课程以促进意义深远的学习(续)

作为大学老师,为了达到好的教学效果,我们必须能够做好两项活动,既要做好课程设计,又要做好师生互动。就这两项活动而言,我们的课程设计能力常常是制约因素。《创造意义深远的学习经历:大学课程整合设计方案》一书作者,美国奥克拉荷马大学教学发展项目部主任迪.芬克博士撰写了一份大学课程设计的自学指南,旨在给大家介绍一套系统有效的课程设计程式,帮助大家学会怎样进行课程设计。指南包括课程整合设计的三个主要阶段,每阶段包括开篇的介绍性评价,读者可以加以使用的工作簿,以及读者在课程设计过程中可以对自己提出的一些问题,以对自己的设计实践进行推敲。     

Extending validity evidence for multidimensional measures of coaching competency

This study extended validity evidence for multidimensional measures of coaching competency derived from the Coaching Competency Scale (CCS; Myers, Feltz, Maier, Wolfe, & Reckase, 2006) by examining use of the original rating scale structure and testing how measures related to satisfaction with the head coach within teams and between teams. Motivation, game strategy, technique, and character building comprised the dimensions of coaching competency. Data were collected from athletes (N = 585) nested within intercollegiate men's (g = 8) and women's (g = 13) soccer and women's ice hockey (g = 11) teams (G = 32). Validity concerns were observed for the original rating scale structure and the predicted positive relationship between motivation competency and satisfaction with the coach between teams. Validity evidence was offered for a condensed post hoc rating scale and the predicted relationship between motivation competency and satisfaction with the coach within teams.     

Three-axis platform simulation: Bond graph and Lagrangian approach

A bond graph model is derived for the geometric constraints of a three-axis flight table. Gimbal dynamics are easily added even in asymmetrical and unbalanced cases. A method is introduced to make the local dependent inertias computable. The bond graph compares favourably to the Lagrangian approach as to modelling effort and accessibility of intermediate variables as well as having computational advantages.     

Infant approach behavior as related to attachment.

The chief purpose of this study was to identify characteristics that distinguish approach as an attachment behavior from approach serving other behavioral systems. Locomotor approaches of 16 male and 10 female white middle-class infants to an attachment figure (the mother) and to a nonattachment figure (the visitor-observer) were examined under naturalistic conditions at home. Observations were made during 4-hour home visits at 3-week intervals; those from 26 to 54 weeks were used. When approach was examined in a free-choice situation and without regard to behavioral context, infants approached the mother proportionally (though slightly) more often than the visitor (p less than .05). In 2 behavioral contexts, however, spontaneous infant approaches were sharply differential to the mother; approaches accompanied by crying and approaches terminating in a pickup appeal were directed almost exclusively to the mother (p less than .0001 for both). Touching upon completion of the approach was not differential, except in the context of a pickup appeal. Object-oriented approaches were more often directed to the visitor than to the mother (p less than .002) and may be either exploratory or affiliative. It is suggested that neither approach nor touching can be assumed to serve the attachment system without consideration of context- both environmental and behavioral.