生物大分子的共振光散射分析

文章介绍了共振光散射技术及其在生物大分子定量分析中的应用,并讨论了其发展方向和前景。     

形象地翻译活动词 实现语篇叙述功能

本文以实现叙述功能的活动词的运用为例 ,通过对两个英语语篇中一些活动的汉语翻译比较 ,揭示出只有形象地翻译活动词 ,才能更好地实现语篇中的叙述功能 ,才能使源语中的活动词在译语中依然体现出它们在语篇中的动态与活力     

How do people read the passages during a reading comprehension test? The effect of reading purpose on text processing behavior

In this study, we investigate the effect of reading purpose on students’ processing behavior during a reading comprehension test. In a repeated measures design, sixty undergraduates answered multiple-choice (MC) reading comprehension questions in a condition with no overarching goal for reading and in an alternate condition where the same students were first provided with the goal of summarizing the text before answering MC questions. Results from eye tracking analysis showed that when students read and answered questions without an overarching goal, they spent much less time reading the passages before answering the questions, more time re-reading the texts while answering the questions, and more time on parts of the text that were not necessary to answer the questions. We conclude that providing examinees without an explicit goal for reading may inadvertently encourage a “search for the answer” reading process, rather than on building a coherent mental model of text content.     

Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.     

信息社会下高等职业院校图书馆服务模式创新研究——以湖州职业技术学院图书馆为例

图书馆是信息资源中心,特别是高等职业院校图书馆,因其馆藏技术性、实用性强,一直在我国职业教育中发挥着举足轻重的作用。信息网络技术的迅猛发展对图书馆信息服务形成了巨大冲击。本文在分析传统图书馆服务弊端、信息化网络化对传统图书馆的冲击以及新形势对图书馆服务新要求的基础上,分析了新形势下图书馆服务的转型方式。并以湖州职业技术学院图书馆转型升级为例,总结了其该图书馆服务创新的举措,说明了其服务创新的效果,分析了仍存在的问题和完善措施,阐述了在信息化网络化的新背景下,图书馆工作人员要以读者中心、以服务为根本,通过提高自身素质、转变服务理念,才能让图书馆更好地发挥其信息资源中心的作用。     

个人知识信息管理的实施过程探析

从个人知识管理的定义出发，分析了知识时个人知识管理的提升作用．最后总结出了个人知识管理具体实施过程的七个方面。     

滚翻垫球后“再后倒”现象的讨论

对滚翻垫球后“再后倒”现象进行理论分析 ,可找出出现问题的根源 ,以便在教学中解决这一问题     

辽代政权中的汉族官僚集团

汉人官僚是辽政权中的一股重要政治力量，在辽的兴衰荣辱中起了重要作用。因此着重就辽政权中汉人官僚的来源、地位及对辽社会的影响做一论述。     

我国信贷传导渠道受阻的原因分析及对策建议

造成信贷传导渠道日趋萎缩的主要原因有二：一是巨大存差的存在；二是资金分布的结构性矛盾。要从根本上解决该问题，政府相关部门、中央银行和商业银行应相互协调，促使信贷传导渠道的疏通。     

基于ASP的WEB数据库安全初探

本文针对当前应用广泛的基于ASP的WEB数据库技术,讨论了WEB数据库的自身安全和ASP的访问问题,并提出了实现基于ASP的WEB数据库网站安全问题的解决策略。