Study on the expression and mutation of human telomeric repeat binding factor ( hTRF1 ) in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells (0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease. Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science Foundation of China (No. 39870339)     

大学校长全球峰会

为了响应联合国秘书长安南提出的“高等院校应该更多地参与国际公共政策和事务”倡议,纽约地区5所高校发起成立了大学校长全球峰会。首届峰会于2005年1月18日至19日在哥伦比亚大学举行,全球著名研究型大学的40多位校长和学者参加了会议,并于6月27日发表了《学术自由宣言》,倡议全球大学校长联合签名,共同创建自己认同的学术自由氛围和环境。     

三羟甲基乙烷和新戊二醇固态相转变贮热的研究

本文用DSC法研究了三羟甲基乙烷（PG）和新戊二醇（NPG）及其混合物的贮热性能，结果表明它们是较好的固-固相变贮热材料．     

对高等学校教学名师评选的分析与思考

高等学校教学名师的评选,对促进高等教育的发展尤其是教育质量的提高具有积极作用。然而笔者在对教育部两届高等学校教学名师的评选结果和评选指标体系进行分析后发现,在评选中,存在着忽视高职高专院校教师的缺陷。对此笔者认为,随着高职教育的蓬勃发展,教学名师评选的指标体系应该有所调整,需要增加高职高专院校候选人名额,或者另外制定一套适合高职高专院校教师的评选指标体系,才能使一批既有较高教学水平,又有行业实践能力的“双师型”教师入选,并通过他们的示范和辐射作用,进一步提高高职高专教师的整体素质和高职高专教育的人才培养质量。     

黑盒软件测试的用例设计技术

主要讨论软件测试中的黑盒测试,介绍了黑盒测试中的四种用例设计技术中的等价类划分和边界值分析,并以两个实际的测试项目对其加以说明。     

柑桔属果皮精油的GC-MS分析 Ⅰ.广西容县沙田柚精油化学成分的研究

以气相色谱-质谱-计算机联用技术研究了广西容县柚皮精油的化学成分,从其总离子流色谱峰鉴定了36个化合物,其中部分倍半萜化合物(萨剔烯、艾里莫芬烷、异可拉酮、长叶蒎烯等)在该精油中为首次发现。     

基于GIS产品的软件测试方法研究

随着信息技术的飞速发展，GIS软件产品已广泛应用于与地理坐标相关的几乎所有领域，软件的规模和复杂度都有很大的提高。因此，提高软件质量及可靠性已成为当前软件工程领域的重要任务．软件测试是保障软件质量的有效手段，面向对象软件测试是面向对象软件开发的不可缺少的一环。笔者从面向对象程序的特点出发，描述如何对GIS软件的分析和设计模型进行测试，强调将软件测试思想融合到软件开发的各个阶段。     

Do rice water weevils and rice stem borers compete when sharing a host plant?

The rice water weevil (RWW) Lissorhoptrus oryzophilus Knsehel (Coleoptera: Curculionidae) is an invasive insect pest office Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis (Walker) (Lepi-doptera: Pyralidae) and pink stem borer Sesamia inferens (Walker) (Lepidoptera: Noctuidae) to rice, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the rice. At tillering stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers (bearing 5 leaves) compared with small tillers (bearing 24 leaves), but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated nega-tively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.     

一种测量钛酸钡/聚氨酯材料电致伸缩性能的新方法

提出利用数字散斑相关方法测量材料的电致伸缩性能的新方法.通过数字散斑相关方法得到面内位移,从而由几何三角关系得到离面位移,实现电致伸缩应变的测量.采用该方法对高电场作用下钛酸钡/聚氨酯复合高分子材料的电致伸缩性能进行了测量.在高电场作用下,对重复加电放电过程进行分析,得到电致伸缩应变,从而获取材料的电致伸缩系数.实验中采用双线性插值方法获取亚象素灰度值,以提高测量精度,并将结果与曲面拟合算法的计算结果进行了比较.结果表明:钛酸钡的加入提高了聚氨酯材料的电致伸缩性能;数字散斑相关方法可以有效地实现材料的电致伸缩系数的测量.该方法为电致伸缩性能的研究提供了一种有效的工具.     

在Authorware中解决重复调用外部文件的方法

重复调用外部程序会占据大量的系统资源,甚至会导致系统不堪重负而崩溃。本文介绍了在Authorware中,如何借助外部扩展函数,通过程序的类名或标题名获得外部程序窗口的句柄,然后用函数操作该句柄,来改变程序窗口的显示状态,从而避免程序被多次同时执行的方法。