中考满分话题作文例谈

2011年中考虽然已经尘埃落定,而且我们哈尔滨市的中考作文没有出现话题作文。可它仍然是我们训练的重点和全国各地中考作文试题的考点。     

精神上的鸢尾花

祖国啊 我亲爱的祖国（节选） 我是你河边上破旧的老水车 数百年来妨着疲惫的歌 我是你颤上熏黑的矿灯 照你在历史的隧洞里娲行摸索 我是千瘪的稻穗;是失修的路基     

一只猫的悲剧

编辑导语 生活中不缺少阿谀,但生活决不能因阿谀而缺少公正的评判;生活里不缺乏温情,但生活绝不能因溺爱而丧失公正的原则。一只猫的悲剧,虽是无奈的畅想,但谁又说这不是在折射我们的生活？     

S.H.E三个天使一台戏

一加一等于二,一加二等于什么?等于一个篱笆三个桩、一个好汉三个帮,等于团结就是力量,SHE。S:Selina,H:Hebe,E:Ella,三个天使般可爱的女孩热热闹闹地组成了"S.H.E",翻译成中文就是"她",她们代表了所有的青春女孩。     

呵护你的眼睛像婴儿

我们都知道眼睛是心灵的窗户,眼睛对于我们来说具有很重要的意义,除了通过这扇窗户了解世界,还要通过眼睛来进行交流。我们总是希望看见一双明亮有神的眼睛,尽管很多女孩子都希望自己有一双大大的眼睛,但事实上,真正能够攫住别人的还是一双明亮有神,自信飞扬的眼睛。     

Study on the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if h TRF1 mutation is one of the factors of the activation oftelomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing.Real-time RT-PCR was used to detect the expression ofhTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of h TRF1 in 10 cell line cells. Results: h TRF1 gene, mapped to 8q 13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108～0.0749) than in normal mononuclear cells (0.0493, 0.0369～0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1,2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.     

挖掘乡土资源 优化思品教学

<正>义务教育思想品德课程标准(2011年版)》(以下简称《课标》)指出,课程资源既包括学校内的教育资源,也包括学校外的各类社会机构和各种教育渠道所蕴含的多种教育资源。教师应建立融合、开放、发展的课程资源观,整合并优化课程资源,充分发挥各种课程资源的人文教育功能,使之为课程实施和教学服务。因此,思想品德教师应认真分析校情和学情,结合《课标》和教材内容要求,学习本地历史,了解     

西汉徙民实边与唐初羁縻政策辨析

纵观中国二千多年的封建社会,中原王朝的兴盛与边疆关系息息相关。历代帝王都极为慎重地采取对策,以安疆强国。历史上出现了素被史家赞誉的汉唐盛世,然考察汉唐两代在对西北边疆问题上,竟采取了两种截然不同的政策,即西汉实行大规模的徙民实边,而唐初则采取羁縻政策,并成为对各少数民族的主要政策。究竟是怎样的历史因素,使汉唐两代在对待同一问题上采取了两种不同的方式以及它们之间有什么关系?本     

长大要签保险单

长大是我曾经最盼望的事,而现在有些迷惑了。我害怕,害怕长大的过程,它使我变得很偏激,甚至对很多事都有点不屑一顾的味道。     

灵感

灵感像流星,从空中滑过哪怕是细微的,我也必须抓住可每一次它都淘气地滑过我的手让我很难触摸到它只在手掌中留下那若有若无的余温灵感是只有半边的图画留给我完成的那一半是多么的美灵感$广州市小北路小学六年级@纯蓝野幻~~