Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.     

Why extraversion is not enough: the mediating role of initial peer network centrality linking personality to long-term academic performance

Academic performance (i.e., grade point average) determines career entry factors as well as career success and is thus crucial for students’ future careers. Besides individual factors such as personality traits, individuals’ social embeddedness has been shown to enhance performance. Regarding academic performance, relationships to fellow students (peers), which bundle into one’s developmental network, are a valuable source of psychosocial and career support because occupying central positions within a social network provides the benefit of being able to access career-enhancing resources. Integrating individual and social-contextual factors for the purpose of examining academic performance is therefore plausible. Research results indicate that personality, especially extraversion, might predict performance through network centrality. In this study, we examined this assumption by focusing on extraversion and centrality in the peer developmental network of recently acquainted German psychology bachelor students (N?=?47, 15% male). In a longitudinal design, we analyzed the impact of extraversion and centrality on students’ academic performance at the end of their studies. Results revealed that centrality (i.e., popularity) mediates the relationship of extraversion with academic performance, indicating that extraverted students (regardless of their agreeableness) are more popular among their peers, which, in turn, enhances their academic performance. That is, the likelihood of getting superior final grades depends on whether students manage to attract peers at the very beginning of their university life, which is easier for extraverts. These findings emphasize the importance of the social embeddedness of people, highlight its long-term effects on performance, and yield several implications for research and practice.     

The contribution of epistemology to curriculum construction in the sciences

Summary The paper focuses upon curriculum planning in the scientific disciplines at university level, although it is claimed the argument may be of wider applicability. Drawing upon the writings of philosophers of education from several decades ago (notably Schwab and Scheffler) whose work is too often overlooked in contemporary debates about the curriculum, and using illustrative examples from the author’s own experience, it is argued that too often the focus of science curriculum planning is the “rhetoric of conclusions” or the “substantive structure” — the current state of knowledge at the forefront of the respective disciplines — to the neglect of what Schwab called the “syntactical structure” of the sciences (which roughly approximates their epistemology). This aspect of these disciplines is essential for the general student trying to become familiar with the nature of science as a broad field of knowledge, for prospective teachers, and — contra Scheffler’s view — for students who aim at careers as researchers.

---

Zusammenfassung Der Beitrag der Epistemologie zur Curriuculumkonstruktion in den Naturwissenschaften Der Aufsatz fokussiert auf die Curriculumplanung für den naturwissenschaftlichen Unterricht in der universit?ren Lehrerausbildung, wenngleich behauptet wird, dass dieses Argument weitreichendere Anwendbarkeit besitzt. Der Text knüpft an erziehungswissenschaftlichen Schriften (insbesondere von Schwab und Scheffler) an, deren Ver?ffentlichung zwar einige Dekaden zurückliegt, deren Beitrag in den aktuellen Debatten aber oft übersehen wird. Darüber hinaus werden einige illustrative Beispiele aus dem Erfahrungsschatz des Autors genutzt, um zu zeigen, dass der Fokus der Curriculumplanung für die Naturwissenschaften — dem augenblicklichen Wissensstand der zu berücksichtigenden Disziplinen zufolge — zu oft in einer „Rhetorik der Schlussfolgerung“ oder „substantivischen Struktur“ besteht, was dazu führt, dass das, was Schwab die „syntaktische Struktur“ der Naturwissenschaften nennt (und ihrer Epistemologie ziemlich nahe kommt), vernachl?ssigt wird. Dieser Aspekt jener Disziplinen ist besonders wichtig für Studierende, die allgemeinbildend vertraut werden m?chten mit den Naturwissenschaften, für angehende Lehrer und — entgegen Schefflers Ansicht — für Studenten, die eine Karriere als Forscher anstreben.

---

---

“When walking in quicksand country, carry a stout pole — it will help you get out should you need to. As soon as you start to sink, lay the pole on the surface of the quicksand. Flop onto your back on top of the pole. Work the pole to a new position: under your hips and at right angles to your spine. Take the shortest route to firmer ground, moving slowly.” Piven/Borgenicht 1999, p. 18

---

This paper was presented at the conference Silence Between the Disciplines, Berlin-Brandenburg Academy of Sciences, Berlin, October 2002.     

Students’ Views Regarding their Participation in University Governance: Implications for Distributed Leadership in Higher Education

The paper examines the views of students regarding the extent of their participation in the management of their university and their satisfaction with the degree of this participation. After an examination of the literature on student participation in university governance, the author presents the results of a survey based on data collected from 135 students of the University of Cyprus in 2002. According to the findings, respondents believed that their involvement in the management of their institution was very limited. This applied to both high and low levels of decision making, even though respondents recognised that their input was greater in less important decisions. The perceived limited involvement resulted in feelings of frustration and dissatisfaction among students, with the majority of respondents demanding a higher level of participation for all three decision making situations considered in the study. The paper discusses the implications of the findings for the practice of distributed leadership at contemporary universities, with emphasis on the need to abandon outdated leadership models. Specifically, it proposes measures for increasing student participation in university governance in the framework of a distributed leadership approach designed to empower the key stakeholders of higher education.     

Connectedness among taiwanese middle school students: a validation study of the hemingway measure of adolescent connectedness

Theories of adolescent connectedness suggest that adolescents strive to become connected by engaging with and valuing the people, activities, and worlds in their social ecology. The purpose of this study was to examine the psychometric properties of a measure designed to assess these worlds of connectedness among 320 junior high school students in Taiwan. The subscales and composite scales evidenced satisfactory reliability and concurrent validity. A hypothesized three-factor, higher order structural model of connectedness was cross-validated. Girls were generally more connected than boys. Both connectedness to school and to friends explained more variance in connectedness to self than did family connectedness. There was mixed support for separation-individuation processes. The measure appears promising in terms of future research on adolescent social development in the Asia Pacific.     

The Effect Teaching Experience Has on Perceived Effectiveness of Interactive Television as a Distance Education Model for Elementary School Science Teacher’s Professional Development: Another Digital Divide?

The first year of a 5 year professional development project for elementary teachers in two mid-western states integrated a bridge of two distinctly different distance education networks (T-1 and fiber optics) to provide science professional development for elementary school teachers in rural communities. Interactive television (ITV), the title given to the distance component, consisted of a series of twenty-four, 2 h presentations by scientists and content specialists. It provided expansion to the science pedagogical content knowledge of the elementary teachers involved. Eighty-five teachers in 38 school districts from the two states completed individual surveys following the final ITV session. Analysis of variance of participants post-session survey scores yielded differences (p < 0.05) on the subscales measuring perceived effectiveness of using distance education for professional development purposes and years of teaching experience. Teachers with over 20 years of classroom experience perceived the use of distance education technologies for science professional development to be more effective than teachers with 16–20 years of classroom experience. These results might suggest another digital divide.     

求解化学反应动力学参数的一种方法

反应级数的确定在化学动力学研究中非常重要,现给出了利用计算机和用微分法确定反应级数的方法.为了使编程的程序简短、高效,采用MATLAB语言作为编程语言.     

Fiber optic biosensor of immobilized firefly luciferase

Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. TheK _m′ for D-luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25°C respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10⁻⁹–10⁻⁵ mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.