CIDE gene expression in adipose tissue,liver, and skeletal muscle from obese and lean pigs

The expression of the cell death-inducing DNA fragmentation factor α-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes’ expression in pigs. Here, we hypothesized that different fat accumulation between lean (Duroc×Landrace×Yorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig. Lantang pigs had higher white adipose and skeletal muscle Cidea and Cidec mRNA abundance, and hepatic and muscle Cideb mRNA than DLY pigs. Lipid metabolism-related genes including sterol regulatory element binding protein 1c (SREBP-1c), hepatocyte nuclear factor-4α (HNF-4α), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), fatty acid synthase (FASN), diacylglycerol O-acyltransferase 1 (DGAT1), and DGAT2 showed a higher expression level in adipose tissue from obese pigs than in that from lean pigs. Lantang pigs exhibited higher mRNA abundance for liver SREBP-1c, HNF-4α, and PGC-1α, and higher skeletal muscle SREBP-1c, HNF-4α, PGC-1α, and DGAT2 expression, as compared with DLY pigs. However, the perlipin2 mRNA levels in adipose tissues, liver, and skeletal muscle were significantly lower in obese pigs than in their lean counterparts. Furthermore, plasma non-esterified fatty acid (NEFA), glucose, and triacylglycerol (TAG) levels were greater in obese pigs than in lean pigs. Finally, data from correlation analysis further found that CIDE mRNA expression was positively correlated with back fat thickness (BFT), abdominal fat mass (AFM), and the levels of NEFA, TAG, and glucose in the two breeds. Collectively, these data revealed that the porcine CIDEs possibly modulated lipid metabolism and contributed to the development of fat deposition and obesity in Lantang pigs.     

The impact of reading at rapid rates on inference generation

Aims

Speed reading is advertised as a way to increase reading speed without any loss in comprehension. However, research on speed reading has indicated that comprehension suffers as reading speed increases. We were specifically interested in how processes of inference generation were affected by speed reading.

Methods

We examined how reading speed influenced inference generation in typical readers, trained speed readers and participants trained to skim read passages. Passages either strongly or weakly promoted a bridging or predictive inference. After reading, participants performed a lexical decision task on either a nonword, neutral or inference‐related word.

Results

Typical readers responded to strong and weak inference words faster than neutral words. There were no statistical differences in reaction time between inference‐related and neutral words for speed and skim readers.

Conclusions

These findings provide no substantive evidence that the appropriate inferences are generated when reading at rapid speeds. Thus, speed reading may be detrimental to normal integrative comprehension processes.     

Successive reversals of a discriminated preference for signaled tailshock

Three rats received unmodifiable tailshock at random intervals in a shuttlebox. In a continuous-choice situation, Ss could choose between an auditory signal immediately preceding or immediately following the tailshock. Over repeated daily 3-h sessions, each S acquired a spatial discrimination indicating a strong preference for the signal preceding tailshock. This preference continued undiminished through two successive reversals of the position associated with signaled shock. This demonstration precludes explanations of the preference-for-signaled-shock phenomenon based upon primary reinforcement value or acquired value of the signal, position preferences, and overt modification of the aversiveness of the reinforcer through such means as postural adjustments. An explanation of recent failures to obtain the preference-for-signaled-shock effect is offered.     

Conversion of CO<Subscript>2</Subscript> to useful substances with composite iron,nickel, and copper catalysts

Composite materials of Fe/Al₂O₃, which consist of small particles of iron supported by thermally stable alumina even at 500–700 °C, have been widely used in the water-gas shift reaction for natural gas reforming. Therefore, Fe/Al₂O₃ is one of the promising candidates for re-transformation of exhausted CO₂ into fuels such as alcohols and hydrocarbons. The development of a CO₂ reforming system using the composite materials of Fe/Al₂O₃ through CO₂ reduction to CO, dissociation of water into hydrogen, and methanol synthesis has been investigated. It was found that dry and steam (i.e. wet) reforming of CO₂ produced almost the same amount of CO. At a temperature above 500 °C, maximal and saturated yields of CO and H₂ from CO₂ and water were obtained. However, this CO₂ reforming system requires higher-pressure conditions from several tens to hundreds standard atmospheric pressure in order to achieve high yield and selectivity for methanol production. In this study we developed the modified CO₂ reforming system by the utilization of Ni and/or Cu instead of Fe in order to obtain other types of useful products such as CO, CH₄, and carbon, more efficiently and selectively under atmospheric pressure. When Ni or Cu was used, conversion of CO₂ was reduced to 76%, while 9% of methane was detected in the case of Ni. On the other hand, though the CO₂ conversion reduced half of the Fe, the selectivity of CO from CO₂ increased to 95% in the case of Cu.     

Numerical investigation of cavitating flow behind the cone of a poppet valve in water hydraulic system

Computational Fluid Dynamics (CFD) simulations of cavitating flow through water hydraulic poppet valves were performed using advanced RNGk-epsilon turbulence model. The flow was turbulent, incompressible and unsteady, for Reynolds numbers greater than 43000. The working fluid was water, and the structure of the valve was simplified as a two dimensional axisymmetric geometrical model. Flow field visualization was numerically achieved. The effects of inlet velocity, outlet pressure, opening size as well as poppet angle on cavitation intensity in the poppet valve were numerically investigated. Experimental flow visualization was conducted to capture cavitation images near the orifice in the poppet valve with 30° poppet angle using high speed video camera. The binary cavitating flow field distribution obtained from digital processing of the original cavitation image showed a good agreement with the numerical result. Project supported by the National Natural Science Foundation of China (No. 59835160) and Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry of China (No. 50175097)     

Operationalizing and validating disciplinary literacy in secondary education

The goal of this study was to define the construct and establish the validity of disciplinary literacy, which has recently gained attention from the implementation of the Common Core State Standards (National Governors Association Center for Best Practices & Council of Chief State School Officers in Common Core State Standards for English language arts & literacy in history/social studies, science, and technical subjects [PDF]. Authors, Washington, DC, 2010). After defining disciplinary literacy in the four core disciplines of English language arts, science, history and social studies, and mathematics, scales were developed and administered to a snowball sample of professionals nationwide, with 857 respondents. The data showed evidence of disciplinary literacy as a multidimensional construct with three related factors: source literacy, analytic literacy, and expressive literacy. Based on EFA and CFA results, we can conclude that there are at least three types of literacy in operation among the four core disciplines. The three factors of literacy varied significantly by the four core disciplines of English/language arts (ELA), science, history and social studies, and mathematics, supporting the notion that each discipline uses literacy uniquely. This is the first study of its kind to attempt to define, quantify, and validate the construct of disciplinary literacy.     

Classifications of college students’ protean and boundaryless orientation to work

Increasingly, individuals navigate their own careers in self-directed (protean) and boundaryless contexts. These concepts have been applied to adult employees but have seen limited application with young adults. Therefore, 205 college students were assessed on the Protean and Boundaryless Career Attitudes scales (Briscoe, Hall, and DeMuth, 2006); i.e., self-directed career management, values-driven, boundaryless mindset, and organizational mobility preferences. Scale items were reworded to focus on college experience. Cluster analysis organized respondents into five distinct protean-boundaryless career types. Three types were similar to those hypothesized for adults, while two types were unique to our sample.     

Filtration of micro-particles within multi-fiber arrays by adhesive DEM-CFD simulation

A 3D multi-time scale discrete element method-computational fluid dynamic (DEM-CFD) coupling approach was applied to investigate the filtration of micron-sized particles by different types of fiber arrays. Both the pressure drop and the filtration efficiency were examined to indicate the filtration performance of the fiber arrays. Fibers that were uniformly arrayed in a parallel or staggered manner were compared. Results showed that the staggered array showed a better performance than the parallel array in terms of both pressure drop and filtration efficiency. Further, we compared the performance of different staggered arrays, i.e. a regular case, one densified in the front layers and another densified in the back layers. The front densified array was found to enter the clogging and cake filtration stage in the shortest time, leading to the highest filtration efficiency, but the highest pressure drop. The back densified array still achieved a much higher filtration efficiency, despite a much lower pressure drop comparable to that of the regular array. The results suggest that the two kinds of densified arrays may be suited for different purposes, e.g. baghouse filters or breathing masks.