Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

DII、esp@cenet、CA在科技查新专利检索中的应用

详细分析德温特专利创新索引(DII)、欧洲专利系统(esp@cenet)、美国化学文摘(CA)3种检索工具对专利文献的报 道特征与检索功能;通过检索实例对3者应用于科技查新专利检索的优势与不足进行评析,并就具体应用方法提出相关建 议。     

科技期刊编辑应善于发掘作者的潜力

从传递交流信息、选择挖掘作者、文稿选题、科研设计、文稿处理、发表后的后续工作等方面论述发掘作者潜力的方式、方法与途径.认为编辑可以而且应该去发掘作者的潜力,这对科技期刊本身、作者、社会都有益处.     

The role of effort in moderating the anxiety-performance relationship: Testing the prediction of processing efficiency theory in simulated rally driving

We tested some of the key predictions of processing efficiency theory using a simulated rally driving task. Two groups of participants were classified as either dispositionally high or low anxious based on trait anxiety scores and trained on a simulated driving task. Participants then raced individually on two similar courses under counterbalanced experimental conditions designed to manipulate the level of anxiety experienced. The effort exerted on the driving tasks was assessed though self-report (RSME), psychophysiological measures (pupil dilation) and visual gaze data. Efficiency was measured in terms of efficiency of visual processing (search rate) and driving control (variability of wheel and accelerator pedal) indices. Driving performance was measured as the time taken to complete the course. As predicted, increased anxiety had a negative effect on processing efficiency as indexed by the self-report, pupillary response and variability of gaze data. Predicted differences due to dispositional levels of anxiety were also found in the driving control and effort data. Although both groups of drivers performed worse under the threatening condition, the performance of the high trait anxious individuals was affected to a greater extent by the anxiety manipulation than the performance of the low trait anxious drivers. The findings suggest that processing efficiency theory holds promise as a theoretical framework for examining the relationship between anxiety and performance in sport.     

Knee extensor and flexor torque development with concentric and eccentric isokinetic training

This study assessed muscular torque and rate of torque development following concentric (CON) or eccentric (ECC) isokinetic training. Thirty-eight women were randomly assigned to either CON or ECC training groups. Training consisted of knee extension and flexion of the nondominant leg three times per week for 20 weeks (SD = 1). Eccentric training increased ECC knee extension and flexion peak torque more than CON training. The ECC group improved acceleration time and time to peak torque with ECC movements versus the CON group. Slow-velocity ECC isokinetic training yielded greater ECC and similar CON torque development gains versus CON training over the course of 20 weeks in young women.     

Short texts to explain problem — Solution structures — and vice versa

Linguistic analysis of short published reports leads to the presentation of an aigorithm that depicts the problem-solving process in terms of a series of evaluative questions. The work shows how reports enable us to define in detail the various stages of problem solving, and it shows that an understanding of these various stages enables us to recognise information structures in written texts; the writing of brief reports of high-priority information on which the whole thinking process is based is thus seen as a vital part of the problem-solving process. One report is analysed in detail to demonstrate the close relationship between information structures in the text and the real-life thought'action process it describes. There is discussion of how paragraphs, sentences and signals within the sentences enable the writer to communicate the information in a conceptual array that represents the actual thought/action process it describes. Educational implications for inter-sentential coherence, discourse analysis, and writing structures, and control of research through written reports are all discussed.     

A control problem with structural choices

The case is considered in which, during the operation of an optimal control system, the optimizer, in addition to applying his usual control, may switch structures. Necessary and sufficient conditions are derived and emphasis is placed on the special characteristics of this problem. Continuous and discrete time set-ups are considered and the separation principle is shown not to hold for the linear quadratic case in the presence of noise.     

Dimensions of R&D location in the United States

R&D activities in the United States, as in other advanced economies, are geographically concentrated in certain types of locations. This study presents data on the location of four dimensions of R&D in the U.S.: industrial R&D laboratories, scientists and engineers engaged in R&D, scientists and engineers employed by the federal government, and research universities. Industrial R&D is much more concentrated in large urban areas than the other dimensions, and appears to locate more in response to the location of manufacturing activity than to the location of research universities and federal research facilities. The location of R&D employment, which includes government university, and industrial employees, is associated with facilities for all three types of R&D. Because of these factors, R&D in the U.S. is found on a significant per capita basis in 44 of 177 urban areas, most of them in the northeastern portion of the country. When two dimensions, industrial R&D laboratories and R&D employees, are combined as a measure of R&D concentration, the locational pattern is less clustered regionally. Ten urban areas in all regions of the U.S. are identified as important complexes of R&D. Since the location of R&D is a major indicator of comparative advantage for technological activities and the economic potential of urban regions, only a few areas of the U.S. are likely to remain important in the generation of innovations.