A three dimensional thermoplastic microfluidic chip for robust cell capture and high resolution imaging

We present a low cost microfluidic chip integrating 3D micro-chambers for the capture and the analysis of cells. This device has a simple design and a small footprint. It allows the implementation of standard biological protocols in a chip format with low volume consumption. The manufacturing process relies on hot-embossing of cyclo olefin copolymer, allowing the development of a low cost and robust device. A 3D design of microchannels was used to induce high flow velocity contrasts in the device and provide a selective immobilization. In narrow distribution channels, the liquid velocity induces a shear stress that overcomes adhesion forces and prevents cell immobilization or clogging. In large 3D chambers, the liquid velocity drops down below the threshold for cell attachment. The devices can be operated in a large range of input pressures and can even be handled manually using simple syringe or micropipette. Even at high flow injection rates, the 3D structures protect the captured cell from shear stress. To validate the performances of our device, we implemented immuno-fluorescence labeling and Fluorescence in Situ Hybridization (FISH) analysis on cancer cell lines and on a patient pleural effusion sample. FISH is a Food and Drug Administration approved cancer diagnostic technique that provides quantitative information about gene and chromosome aberration at the single cell level. It is usually considered as a long and fastidious test in medical diagnosis. This process can be easily implanted in our platform, and high resolution fluorescence imaging can be performed with reduced time and computer intensiveness. These results demonstrate the potential of this chip as a low cost, robust, and versatile tool adapted to complex and demanding protocols for medical diagnosis.     

A perspective on optical developments in microfluidic platforms for Caenorhabditis elegans research

Microfluidics offers unique ways of handling and manipulating microorganisms, which has particularly benefited Caenorhabditis elegans research. Optics plays a major role in these microfluidic platforms, not only as a read-out for the biological systems of interest but also as a vehicle for applying perturbations to biological systems. Here, we describe different areas of research in C. elegans developmental biology and behavior neuroscience enabled by microfluidics combined with the optical components. In particular, we highlight the diversity of optical tools and methods in use and the strategies implemented in microfluidics to make the devices compatible with optical techniques. We also offer some thoughts on future challenges in adapting advancements in optics to microfluidic platforms.     

Groupitizing reflects conceptual developments in math cognition and inequities in math achievement from childhood through adolescence

Understanding the cognitive processes central to mathematical development is crucial to addressing systemic inequities in math achievement. We investigate the “Groupitizing” ability in 1209 third to eighth graders (mean age at first timepoint = 10.48, 586 girls, 39.16% Asian, 28.88% Hispanic/Latino, 18.51% White), a process that captures the ability to use grouping cues to access the exact value of a set. Groupitizing improves each year from late childhood to early adolescence (d = 3.29), is a central predictor of math achievement (beta weight = .30), is linked to conceptual processes in mathematics (minimum d = 0.69), and helps explain the dynamic between the ongoing development of non-symbolic number concepts, systemic educational inequities in school associated with SES, and mathematics achievement (minimum beta weight = .11) in ways that explicit symbolic measures may miss.     

Relationship between split-step timing and leg stiffness in world-class tennis players when returning fast serves

ABSTRACT

This study investigated split-step timing when returning serves and whether this timing is related to neuromechanical capabilities in world-class tennis players. In Experiment 1, the split-step timing of four groups of world-class players (male and female ATP/WTA seniors, and ITF juniors) was measured on court when returning serves of the same level players. The four groups initiated the split-step at a similar time, starting around the time ball-racket contact in the serve stroke and landed when early ball-flight information was available. In Experiment 2, the neuromechanical capabilities (leg stiffness and vertical jump performance) of a group of world-class players and three groups of less-skilled tennis players were examined. The results showed an increase in leg stiffness with an increase in the level of expertise. A cross-experiment analysis in world-class male players (ATP/ITF, n = 10) revealed that the timing of initial foot movement was significantly correlated with the leg stiffness (r² = 0.54), with later lateral step after the serve in the players who had higher stiffness. The findings support the hypothesis that world-class tennis players adapt perceptual-motor control on the basis of their neuromechanical capabilities and maximise the time before initiating their interceptive action to rely on more reliable information.     

How does the tennis serve technique influence the serve-and-volley?

In tennis, a high ball velocity and a fast run toward the net are key features to successful performance of "serve-and-volley" players. For the serve, tennis players can use two techniques: the foot-up (FU) or foot-back (FB) technique. The aim of this study was to determine if the running time toward the net after the serve and the ball velocity (V(ball)) vary between these two techniques. Moreover we analysed the angular momentum values of the trunk and of the arm holding the racquet. Fifteen expert tennis players performed six successful serve-and-volleys with both techniques. Running time to the net is significantly lower for FB, whereas V(ball) is significantly higher for FU. Trunk and arm angular momentums about the transverse axis are significantly higher with FU before ball impact. A significant correlation (r = 0.81, P < 0.001) exists between changes in the maximal trunk angular momentum and in running time to the net between the two serve techniques. A significant correlation (r = 0.84, P < 0.001) also exists between changes in the maximal trunk angular momentum and in V(ball) between the two serve techniques. According to these results, FB is the best technique for moving as quickly as possible to the net because of a lower trunk angular momentum.     

The prognostic relevance of psychological factors with regard to participation and success in table-tennis

ABSTRACT

This study examined the prognostic relevance of self-determined motivation, coping, burnout, perceived stress and recovery experienced by 159 youth table-tennis players involved in intensive training centers with regard to their participation and success six years later. Results of ANCOVAs showed that players who still practiced at time 2 (T2; six years later; n = 130) reported lower time 1 (T1; while they were involved in intensive training centers) amotivation (large effect), disengagement-oriented coping, sport devaluation and reduced accomplishment (moderate effects) than their counterparts who dropped out at T2 (n = 29). Results of ANCOVAs also showed that international (n = 18) and/or national players (n = 86) at T2 reported significantly lower T1 amotivation (large effect), disengagement-oriented coping and sport devaluation (moderate effects) in comparison to regional (n = 26) players at T2. Finally results of correlational analyses showed that T2 performance and/or six-year performance progress were significantly and weakly correlated with introjected and external regulations, perceived stress and perceived recovery, and significantly and moderately correlated with amotivation, disengagement-oriented coping, sport devaluation, and reduced accomplishment. Overall, this study provided insights into the role played by self-determined motivation, coping, burnout, perceived stress and recovery in the table-tennis players’ dropout and performance level six years later.     

Changes in student effort and grade expectation in the course of a term

Students were surveyed and asked to self-evaluate their performance and time-on-task in six engineering courses (eight sections). Surveys were conducted four times over the course of a 10-week quarter. Students with the highest grade point averages (GPAs) (2.88 mean; 4.00 mode) indicated ‘no change’ while students with lower GPAs (2.67 mean; 1.83 mode) indicated a ‘negative change’ in grade expectations by the end of the quarter, indicating that better performing students are better self-evaluators. Students reduced time-on-task 2–4 hours/week, especially early in the quarter. Students who changed their time-on-task during each survey had a GPA mode of 4.00, indicating that ‘master’ students readily adapt to course demands. This study shows that most students over-predict their grades and their level of commitment to a course and lose confidence in their abilities as the term progresses. It is suggested that instructors obtain student grade predictions and use them to provide timely and appropriate feedback.     

Time courses of emotions experienced after a mountain ultra-marathon: Does emotional intelligence matter?

This research examined the time courses of emotions in sport settings (anxiety, dejection, anger, happiness, excitement) experienced by mountain ultra-marathon (MUM) runners within the month following a demanding MUM race and the role of emotional intelligence (EI) in these time courses. A six-wave one-month longitudinal design was used with one measurement point within two days before the race to measure EI and five time points within the month following the race to assess emotions experienced among a sample of 29 runners. Results of multilevel growth curve analyses showed significant linear decreases of dejection and anxiety and a significant linear increase of anger. EI was related to the intercept (level at the end of the MUM race) of happiness, excitement and dejection. Moreover the interaction of EI with time was associated with happiness, excitement and anger. This means that high and low emotional intelligent runners exhibited distinct trajectories of emotional intelligence within the month following the MUM race. Indeed, trait-EI appeared to have a protective role against stress process leading to emotional adjustment within the recovery period following an ultra-endurance event. As such, consultants and coaches could conduct specific program over the sport season designed to enhance trait-EI of MUM runners.     

The effects of player grip on the dynamic behaviour of a tennis racket

The aim of this article is to characterise the extent to which the dynamic behaviour of a tennis racket is dependent on its mechanical characteristics and the modulation of the player’s grip force. This problem is addressed through steps involving both experiment and modelling. The first step was a free boundary condition modal analysis on five commercial rackets. Operational modal analyses were carried out under “slight”, “medium” and “strong” grip force conditions. Modal frequencies and damping factors were then obtained using a high-resolution method. Results indicated that the dynamic behaviour of a racket is not only determined by its mechanical characteristics, but is also highly dependent on the player’s grip force. Depending on the grip force intensity, the first two bending modes and the first torsional mode frequencies respectively decreased and increased while damping factors increased. The second step considered the design of a phenomenological hand-gripped racket model. This model is fruitful in that it easily predicts the potential variations in a racket’s dynamic behaviour according to the player’s grip force. These results provide a new perspective on the player/racket interaction optimisation by revealing how grip force can drive racket dynamic behaviour, and hence underlining the necessity of taking the player into account in the racket design process.