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934.
In vitro sensitivity testing of tumor cells could rationalize and improve the choice of chemotherapy and hormone therapy. In this report, a microfluidic device made from poly(dimethylsiloxane) and glass was developed for an assay of drug induced cytotoxicity. We evaluated the apoptotic and proliferation-inhibitory effects of anticancer drugs mitomycin C (MMC) and tamoxifen (TAM) using MCF-7 breast cancer cells. MMC and TAM both induced apoptosis and inhibited proliferation of MCF-7 cells in a concentration-dependent manner. MMC caused the expression of antiapoptotic protein Bcl-2 a dose-dependent reduction in MCF-7 cells. The expression of Bcl-2 did not change significantly in MCF-7 cells treated by TAM. The results in the microfluidic device were correlated well with the data obtained from the parallel experiments carried out in the conventional culture plates. The developed microfluidic device could be a potential useful tool for high content screening and high throughput screening research. 相似文献
935.
Schmolke H Demming S Edlich A Magdanz V Büttgenbach S Franco-Lara E Krull R Klages CP 《Biomicrofluidics》2010,4(4):44113
Polyelectrolyte multilayers (PEMs) based on the combinations poly(diallyldimethylammonium chloride)∕poly(acrylic acid) (PDADMAC∕PAA) and poly(allylamine hydrochloride)∕PAA (PAH∕PAA) were adsorbed on poly(dimethylsiloxane) (PDMS) and tested for nonspecific surface attachment of hydrophobic yeast cells using a parallel plate flow chamber. A custom-made graft copolymer containing poly(ethylene glycol) (PEG) side chains (PAA-g-PEG) was additionally adsorbed on the PEMs as a terminal layer. A suitable PEM modification effectively decreased the adhesion strength of Saccharomyces cerevisiae DSM 2155 to the channel walls. However, a further decrease in initial cell attachment and adhesion strength was observed after adsorption of PAA-g-PEG copolymer onto PEMs from aqueous solution. The results demonstrate that a facile layer-by-layer surface functionalization from aqueous solutions can be successfully applied to reduce cell adhesion strength of S. cerevisiae by at least two orders of magnitude compared to bare PDMS. Therefore, this method is potentially suitable to promote planktonic growth inside capped PDMS-based microfluidic devices if the PEM deposition is completed by a dynamic flow-through process. 相似文献
936.
那时花开,流水芳年。
青春很锋利,很容易就划开记忆的容颜,岁月如梭,稍纵即逝,我们还没来得及回忆青涩和苦涩,感受阵痛和欣喜,时间就呼啸而过了。 相似文献
937.
938.
第二章神秘娱乐的层次关系
从我们的这边看出去,我们带给观众的表演是魔术效果(effect)。而从观众的角度来看呢,我们的演出可分成三种不同的感受: 相似文献
939.
Miyamoto N Wakahara T Sugisaki N Murata K Kanehisa H Fukunaga T Kawakami Y 《Journal of sports sciences》2010,28(14):1535-1542
The aim of the present study was to examine elbow joint extension power-load characteristics with and without a countermovement. Eight male participants performed maximal elbow extensions with and without a countermovement against different loads (0, 2.5, 5.0, 7.5, 10.0, 12.5, and 15.0 kg). Electromyographic activity of the lateral and long heads of the triceps brachii and the biceps brachii muscles was recorded. The average joint power in the concentric phase was significantly enhanced in the countermovement condition at all loads except for 0 kg. The optimal load for the maximal joint power was greater in the countermovement (7.5 kg) than in the no countermovement condition (5.0 kg). Electromyographic activity was unchanged over the intensities and conditions. Our results suggest that the optimal load for the maximal joint power depends on the type of action (i.e. with or without a counter-movement), and that the enhanced joint power in the countermovement condition is due primarily to the storage and utilization of elastic energy. 相似文献
940.
Kinematic differences of elite and high-performance tennis players in the cross court and down the line forehand 总被引:1,自引:0,他引:1
Landlinger J Lindinger SJ Stöggl T Wagner H Müller E 《Sports biomechanics / International Society of Biomechanics in Sports》2010,9(4):280-295
This study identified and compared the full body kinematics of different skill levels in the forehand groundstroke when balls were hit cross court and down the line. Forty-three three-dimensional retroreflective marker trajectories of six elite and seven high-performance players were recorded using an eight-camera 400 Hz, Vicon motion analysis system. The six highest horizontal velocity forehands with reliable kinematics of all participants were analysed for each specific situation (a total of 156 analysed shots). Significant differences (p < 0.01) and large effect sizes were observed between elite and high-performance players in linear velocity of the shoulder (2.0 vs. 1.2 m/s), angular velocity of the pelvis (295 vs. 168 degrees/s), and angular velocity of the upper trunk (453 vs. 292 degrees/s) at impact. The elite group showed a tendency towards higher racquet velocities at impact (p < 0.05). No significant differences were found in angular displacement of the racquet, hip alignment, or shoulder alignment at the completion of the backswing; nor did angular displacement vary significantly at impact. Irrespective of the group, different shoulder, hip, and racquet angles were found at impact, depending on the situation. The results should assist coaches when striving to improve their players' forehand. 相似文献