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61.
The objective of this study was to evaluate acrosin activity in spermatozoa of infertile Nigerian men and to compare with
those of the fertile men. The acrosin activity was evaluated using the Acroscreen reagent kit. The acrosin activity of the
fertile men was 40.7±5.2 mIU/106 sperm (range 30.1–51.3) and those of the infertile men was 22.4±8.33 mIU/106 sperm (range 5.7–39.1). The difference in the mean was statistically significant (P<0.001). There was a subpopulation of
the infertile men who had their acrosin activity within normal range of the fertile men, 32.9±1.57 mIU/106 sperm. But the difference in the mean was statistically significant (P<0.001). Acrosin activity decreased with increased
morphological changes in the spermatozoa. It is concluded that acrosin activity in the infertile Nigerian men is significantly
lower than that in the fertile men. Acrosin activity may also be affected by morphological changes in the spermatozoa. 相似文献
62.
The Inductive Query By Example (IQBE) paradigm allows a system to automatically derive queries for a specific Information Retrieval System (IRS). Classic IRSs based on this paradigm [Smith, M., & Smith, M. (1997). The use of genetic programming to build Boolean queries for text retrieval through relevance feedback. Journal of Information Science, 23(6), 423–431] generate a single solution (Boolean query) in each run, that with the best fitness value, which is usually based on a weighted combination of the basic performance criteria, precision and recall. 相似文献
63.
B. G. C. Maisonneuve T. Honegger J. Cordeiro O. Lecarme T. Thiry D. Fuard K. Berton E. Picard M. Zelsmann D. Peyrade 《Biomicrofluidics》2016,10(2)
With the rise of microfluidics for the past decade, there has come an ever more pressing need for a low-cost and rapid prototyping technology, especially for research and education purposes. In this article, we report a rapid prototyping process of chromed masks for various microfluidic applications. The process takes place out of a clean room, uses a commercially available video-projector, and can be completed in less than half an hour. We quantify the ranges of fields of view and of resolutions accessible through this video-projection system and report the fabrication of critical microfluidic components (junctions, straight channels, and curved channels). To exemplify the process, three common devices are produced using this method: a droplet generation device, a gradient generation device, and a neuro-engineering oriented device. The neuro-engineering oriented device is a compartmentalized microfluidic chip, and therefore, required the production and the precise alignment of two different masks. 相似文献
64.
Gergely Simon Caroline Busch Marco A. B. Andrade Julien Reboud Jonathan M. Cooper Marc P. Y. Desmulliez Mathis O. Riehle Anne L. Bernassau 《Biomicrofluidics》2021,15(1)
Separation and sorting of biological entities (viruses, bacteria, and cells) is a critical step in any microfluidic lab-on-a-chip device. Acoustofluidics platforms have demonstrated their ability to use physical characteristics of cells to perform label-free separation. Bandpass-type sorting methods of medium-sized entities from a mixture have been presented using acoustic techniques; however, they require multiple transducers, lack support for various target populations, can be sensitive to flow variations, or have not been verified for continuous flow sorting of biological cells. To our knowledge, this paper presents the first acoustic bandpass method that overcomes all these limitations and presents an inherently reconfigurable technique with a single transducer pair for stable continuous flow sorting of blood cells. The sorting method is first demonstrated for polystyrene particles of sizes 6, 10, and 14.5 μm in diameter with measured purity and efficiency coefficients above 75 ± 6% and 85 ± 9%, respectively. The sorting strategy was further validated in the separation of red blood cells from white blood cells and 1 μm polystyrene particles with 78 ± 8% efficiency and 74 ± 6% purity, respectively, at a flow rate of at least 1 μl/min, enabling to process finger prick blood samples within minutes. 相似文献
65.
Mamatha T. Shenoy Benedicta D’Souza Lalesh Naik Akshatha Vivian D’Souza Madan Gopal Rajan 《Indian journal of clinical biochemistry : IJCB》2015,30(3):360-362
Tumor lysis syndrome has been observed in patients with malignancies with high cellular burden and high cell turnover, tumor sensitive to therapy, especially after initiating medical treatment. It very rarely occurs spontaneously. The case described here is of 6 months male infant who presented with fever since 1 month and loose stools associated with blood since 15 days. The laboratory investigations showed lactate dehydrogenase (LDH) of 6,192 IU/L and serum uric acid 18.2 mg/dl along with pancytopenia. The infant presented with electrolyte abnormalities and renal failure. 相似文献
66.
The purpose of this paper is to compute the Hankel transform Fn(y) of order n of a function f(x) and its inverse transform using rationalized Haar wavelets. The integrand is replaced by its wavelet decomposition. Thus representing Fn(y) as a Fourier-Bessel series with coefficients depending strongly on the local behavior of the function , thereby getting an efficient algorithm for their numerical evaluation. Numerical evaluations of test functions with known analytical Hankel transforms illustrate the proposed algorithm. 相似文献
67.
68.
In this paper, the problem of stability analysis for neural networks with time-varying delays is considered. By the use of a newly augmented Lyapunov functional and some novel techniques, sufficient conditions to guarantee the asymptotic stability of the concerned networks are established in terms of linear matrix inequalities (LMIs). Three numerical examples are given to show the improved stability region of the proposed works. 相似文献
69.
Nam Cao Hoai Le Murat Gel Yonggang Zhu Jian Wang Helen Dacres Alisha Anderson Stephen C. Trowell 《Biomicrofluidics》2014,8(6)
Bioluminescence resonance energy transfer (BRET) is a form of Förster resonance energy transfer. BRET has been shown to support lower limits of detection than fluorescence resonance energy transfer (FRET) but, unlike FRET, has not been widely implemented on microfluidic devices for bioanalytical sensing. We recently reported a microscope-based microfluidic system for BRET-based biosensing, using a hybrid, high quantum-efficiency, form of BRET chemistry. This paper reports the first optical fiber-based system for BRET detection on a microfluidic chip, capable of quantifying photon emissions from the low quantum-efficiency BRET2 system. We investigated the effects of varying core diameter and numerical aperture of optical fibers, as well as varying microfluidic channel design and measurement conditions. We optimized the set-up in order to maximize photon counts and minimize the response time. The optimized conditions supported measurement of thrombin activity, with a limit of detection of 20 pM, which is lower than the microscope-based system and more than 20 times lower than concentrations reported to occur in plasma clots. 相似文献
70.
利用双标图分析基因型和环境交互作用 总被引:28,自引:0,他引:28
在作物的品种区域化试验中,品种的基因型和环境(G×E)交互作用越来越受到重视.一种新的非常有效的分析G×E交互作用模型是加性主效应和乘积交互作用模型(简称AMMI模型),而双标图(biplot)是解释AMMI分析结果的一种直观有效的图形工具.本文对AMMI模型及双标图作一扼要介绍,并选用1995年南方水稻区试单季晚粳组数据作为实例,给出了两种新的双标图,以帮助我们更深入地了解G×E交互作用. 相似文献