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671.
We report how cell rheology measurements can be performed by monitoring the deformation of a cell in a microfluidic constriction, provided that friction and fluid leaks effects between the cell and the walls of the microchannels are correctly taken into account. Indeed, the mismatch between the rounded shapes of cells and the angular cross-section of standard microfluidic channels hampers efficient obstruction of the channel by an incoming cell. Moreover, friction forces between a cell and channels walls have never been characterized. Both effects impede a quantitative determination of forces experienced by cells in a constriction. Our study is based on a new microfluidic device composed of two successive constrictions, combined with optical interference microscopy measurements to characterize the contact zone between the cell and the walls of the channel. A cell squeezed in a first constriction obstructs most of the channel cross-section, which strongly limits leaks around cells. The rheological properties of the cell are subsequently probed during its entry in a second narrower constriction. The pressure force is determined from the pressure drop across the device, the cell velocity, and the width of the gutters formed between the cell and the corners of the channel. The additional friction force, which has never been analyzed for moving and constrained cells before, is found to involve both hydrodynamic lubrication and surface forces. This friction results in the existence of a threshold for moving the cells and leads to a non-linear behavior at low velocity. The friction force can nevertheless be assessed in the linear regime. Finally, an apparent viscosity of single cells can be estimated from a numerical prediction of the viscous dissipation induced by a small step in the channel. A preliminary application of our method yields an apparent loss modulus on the order of 100 Pa s for leukocytes THP-1 cells, in agreement with the literature data. 相似文献
672.
Iñigo Mujika Matt Spencer Juanma Santisteban Juan José Goiriena David Bishop 《Journal of sports sciences》2013,31(14):1581-1590
Abstract In this study, we investigated the age-related differences in repeated-sprint ability and blood lactate responses in 134 youth football players. Players from the development programme of a professional club were grouped according to their respective under-age team (U-11 to U-18). Following familiarization, the participants performed a repeated-sprint ability test [6 × 30-m sprints 30 s apart, with active recovery (2.0–2.2 m · s?1) between sprints]. The test variables were total time, percent sprint decrement, and post-test peak lactate concentration. Total time improved from the U-11 to U-15 age groups (range 33.15 ± 1.84 vs. 27.25 ± 0.82 s), whereas no further significant improvements were evident from U-15 to U-18. No significant differences in percent sprint decrement were reported among groups (range 4.0 ± 1.0% to 5.5 ± 2.1%). Post-test peak lactate increased from one age group to the next (range 7.3 ± 1.8 to 12.6 ± 1.6 mmol · l?1), but remained constant when adjusted for age-related difference in body mass. Peak lactate concentration was moderately correlated with sprint time (r = 0.70, P > 0.001). Our results suggest that performance in repeated-sprint ability improves during maturation of highly trained youth football players, although a plateau occurs from 15 years of age. In contrast to expectations based on previous suggestions, percent sprint decrement during repeated sprints did not deteriorate with age. 相似文献
673.
Gaston P. Beunen† Robert M. Malina Duarte L. Freitas Martine A. Thomis José A. Maia Albrecht L. Claessens 《Journal of sports sciences》2013,31(15):1683-1691
Abstract The purpose of this study was to validate and cross-validate the Beunen-Malina-Freitas method for non-invasive prediction of adult height in girls. A sample of 420 girls aged 10–15 years from the Madeira Growth Study were measured at yearly intervals and then 8 years later. Anthropometric dimensions (lengths, breadths, circumferences, and skinfolds) were measured; skeletal age was assessed using the Tanner-Whitehouse 3 method and menarcheal status (present or absent) was recorded. Adult height was measured and predicted using stepwise, forward, and maximum R 2 regression techniques. Multiple correlations, mean differences, standard errors of prediction, and error boundaries were calculated. A sample of the Leuven Longitudinal Twin Study was used to cross-validate the regressions. Age-specific coefficients of determination (R 2) between predicted and measured adult height varied between 0.57 and 0.96, while standard errors of prediction varied between 1.1 and 3.9 cm. The cross-validation confirmed the validity of the Beunen-Malina-Freitas method in girls aged 12–15 years, but at lower ages the cross-validation was less consistent. We conclude that the Beunen-Malina-Freitas method is valid for the prediction of adult height in girls aged 12–15 years. It is applicable to European populations or populations of European ancestry. 相似文献
674.
Ricardo Mora-Custodio David Rodríguez-Rosell Juan Manuel Yáñez-García Miguel Sánchez-Moreno Fernando Pareja-Blanco Juan José González-Badillo 《Journal of sports sciences》2013,31(24):2856-2864
ABSTRACTThis study aimed to analyze the acute effect of inter-repetition rest (IRR) intervals on mechanical and metabolic response during four resistance exercise protocols (REPs). Thirty resistance-trained men were randomly assigned to: continuous repetitions (CR), 10 s (IRR10) or 20 s (IRR20) inter-repetition rest. The REPs consisted of 3 sets of 6, 5, 4 and 3 repetitions against 60, 70, 75 and 80% 1RM, respectively, in the full squat exercise. Muscle fatigue was assessed using: percentage of velocity loss over three sets, percentage of velocity loss against the ~1 m·s?1 load (V1 m·s?1), and loss of countermovement jump (CMJ) height pre-post exercise. Blood lactate was measured before and after exercise. The percentage of velocity loss over three sets and lactate concentration were significantly lower (P < 0.05) for IRR groups compared to CR in all REPs. The CR group showed a significantly higher (P < 0.05) velocity loss against V1 m·s?1 load and loss of CMJ height pre-post exercise than IRR groups in REP against 60% 1RM. In conclusion, both IRR groups produced a significant lower degree of fatigue compared to CR group. However, no significant differences were found in any measured variables between IRR configurations. 相似文献
675.
676.
Fernando Ribeiro Fernando Santos Pedro Gonçalves José Oliveira 《European Journal of Sport Science》2013,13(6):397-402
Abstract It has been suggested that a greater number of injuries during the last third of practice sessions or matches could be related to alteration of lower limb neuromuscular control due to neuromuscular fatigue. This fatigue-related effect can be mediated by changes in joint proprioception. In athletes, the use of functional fatigue protocols could have the advantage of mimicking the demands of sporting activity, thus reflecting more specifically the changes in neuromuscular control and proprioception observed in sport settings. The purpose of the present study was to assess the effect of fatigue induced by a volleyball match on knee joint position sense in elite volleyball players. Seventeen female volleyball players aged 18.9±4.2 years from the Portuguese national team volunteered for the study. Knee joint position sense was evaluated by an open kinetic chain technique and active knee positioning, and was reported using absolute, relative, and variable angular errors. Joint position sense measures were obtained before and immediately after a simulated volleyball match of five sets. To ensure that the match was sufficiently intense to induce fatigue, the perceived exertion or exercise intensity was assessed at the end of the match using Borg’ s rating of perceived exertion (RPE) scale. All participants completed the volleyball match (90min duration), reaching or exceeding 15 on the RPE scale (15.59±0.71; range 15–17), equivalent to “hard/heavy work”. After the volleyball match, a significant increase in absolute (2.11°) and relative (1.71°) angular errors was detected. Match-induced fatigue significantly exacerbated the overestimation of the test position. Moreover, the reliability and accuracy in estimating knee angles decreased from rest to the fatigued state, as shown by the increase (264%) in variable error. Our results show that fatigue induced by a volleyball match has a marked effect on knee joint position sense in elite female volleyball players. Knee joint position sense was less accurate and less consistent after the volleyball match. Fatigue induced by a simulated competitive volleyball match resulted in proprioceptive deficits, decreasing sensorimotor system acuity in female volleyball players. 相似文献
677.
In this study, we examined the relationships between intrinsic motivation, physical self-concept, and satisfaction with life using cross-lagged panel models analysed via structural equation models. The sample consisted of 293 participants (148 men, 145 women) aged 18-70 years who performed diverse types of physical exercise. Three alternative models were tested. The data were better represented by a model in which physical self-concept mediates the relationships between intrinsic motivation and satisfaction with life. Specifically, the direct effects of intrinsic motivation on physical self-concept, and of physical self-concept on satisfaction with life were significant, with the amount of explained variance increasing over time. In conclusion, people who perform physical exercise regularly, and who have fun over time will tend to have a better physical self-perception and, consequently, more psychological well-being. 相似文献
678.
José Ignacio Rojas-Sola Miguel Castro-García María del Pilar Carranza-Cañadas 《Journal of Cultural Heritage》2012,13(3):285-292
This article presents new research, which has the objective of quantifying the knowledge produced by historical technological innovations in the field of olive oil industrial heritage. It studies inventions related to the mechanical processes of the extraction of olive oil between 1826 and 1966, which are recorded in the Historical Archive of the Spanish Patent and Trademark Office. It uses a new adaptation of the methodology of knowledge management (KM) defined by Nonaka and Takeuchi. We have obtained the knowledge value of each innovation according to the level of technological knowledge registered at the time, while distinguishing between the historical evolution of inventions relating to milling and those relating to pressing. In the first analysis, the results show a recession in the quantitative level of knowledge in the olive oil sector with respect to the technological potential of the time. However, when contrasted with the events relating to industrial heritage at the time, it is possible to account for this technological evolution and prove the validity of the methodology used. The results show that general evolution of the knowledge generated has decreased, particularly in the pressing process, although this is not the case in the milling process. 相似文献
679.
680.
Frans Kaiser Jos B.J. Koelman Raymond J.G.M. Florax Frans A. van Vught 《Higher Education in Europe》1992,17(1):33-64
This article is an exercise in cross‐national comparative research on the financing of higher education, the countries involved being the EC countries. Before relevant comparisons could be made, agreement had to be reached as to what “higher education” is and what “students” are. It was then necessary to find some way of making accurate comparisons of monetary units and of dealing with contradictory ways in which educational statistics were and still are collected and collated. A research strategy was developed using existing sources, a specially developed questionnaire, and consultation with national experts. The results displayed biases for which corrections had to be made. They also, however, displayed a wide variety in the level of higher education expenditure in the EC countries. The project gave rise to the creation of the first comprehensive database that covers public expenditure on higher education in the twelve member states of the EC. It can be regarded as the first step in the creation of the “perfect database” on higher education expenditure which in turn would be a most valuable tool for higher education policy‐makers.