响应面法优化解酯假丝酵母Candida lipolytica产脂肪酶发酵条件

用响应面法对Candida lipolytica液体发酵产脂肪酶的发酵条件进行了快速优化。首先运用逐因子试验确定Candida lipolytica产脂肪酶的最佳碳源和氮源分别为葡萄糖和蛋白胨。在此基础上,通过Plackett-Burman设计对影响其产酶相关因素进行评估并筛选出具有显著效应的三个因素:蛋白胨,KH2PO4,初始pH。在用最陡爬坡试验逼近以上三个因子的最大响应区域后,采用响应面分析法,确定其最优发酵条件:葡萄糖0.250%,蛋白胨0.600%,KH2PO40.603%,KH2PO40.100%,MgSO4·7H2O0.050%,橄榄油0.500%,吐温-800.500%,初始pH值为7.85,转速为180r/min,28℃培养48h。最终优化后的酶活达到208.9U/ml,比初始酶活91.3U/ml提高了129%。     

地方科研机构全面转制的操作策略探索

要为地方科研机构深化科技体制改革创造良好的氛围和制定有利的相关配套政策,并采取切实可行的操作策略及方法,积极推进地方科研机构的全面转制。     

Running a TEMPUS Project: First Experiences

This paper describes the experiences gathered at Aachen University's computer science department while running one of the first TEMPUS projects. An outline of the historical background is followed by a brief introduction to the TEMPUS programme as such. The aims and goals of our project, BASCULE, are then described in more detail. Finally, we discuss the lessons we have learned.