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421.
Twenty isolates ofMycobacterium tuberculosis resistant to rifampicin(RIF), isoniazid(INH) and streptomycin(STR) were analysed by Polymerase Chain Reaction (PCR) amplification
of rpoB, katG and rrs genes to evaluate comparative diagnostic significance of genetic assays. Mutations were identified by
single strand conformation polymorphism (SSCP) and cleavase fragment length polymorphism (CFLP) and were confirmed by DNA
sequencing. SSCP of 4 RIF resistant and 14 INH resistant isolates showed an extra peak at the level of 75-bp and 85-bp respectively,
while 2 STR resistant isolates showed 2 peaks with 9 bases difference. CFLP showed a different pattern among RIF, INH and
STR sensitive and resistant isolates Thus SSCP and CFLP can be used as alternative diagnostic methods for identification of
mutations in RIF, INH and STR resistant strains of M.tuberculosis. 相似文献
422.
Brandon Patterson Lauren Clark Ana C. Sanchez-Birkhead Liliana I. Martinez Marlene J. Egger 《Journal of the Medical Library Association》2021,109(4):667
Background:Few electronic resources are available for new mothers with concerns about changes in their pelvic floor following childbirth. Patients may struggle when seeking authoritative information regarding pelvic floor conditions online given the sensitivity of the topic as well as the inadvertent connection to obscene or demeaning content found online. A health sciences librarian partnered with the Motherhood and Pelvic Health Study, an interdisciplinary research group, to provide expert searching skills for a particularly challenging health condition that patients struggle to find useful information on.Case Presentation:A custom rubric was developed to evaluate existing information products, which included criteria for cultural sensitivity, conflicts of interest, and other red flags. This evaluation process enabled the research team to identify top-tier evidence-based materials that were culturally congruent. This collaborative evaluation process led to the creation of a web-based toolkit resource for new mothers concerned about changes in their pelvic floor. The toolkit connects women to pertinent information on a national health organization''s patient portal, supplemented by videos created by the team to serve as models of communication for women and health care providers.Conclusion:When developing a web-based resource, health sciences libraries can partner with research teams to find, evaluate, and disseminate information. Culturally congruent toolkits such as this one can improve access to health information and lead to improved health outcomes. To ensure that the information highlighted in toolkits is both culturally congruent and authoritative, research teams should form advisory committees and partner with relevant professional medical associations. 相似文献
423.
Francisco José Collado-Montero Ana Isabel Calero-Castillo Manuel Melgosa Víctor Jesús Medina 《文物保护研究》2013,58(2):90-100
ABSTRACTThe main aim of our current investigation is the colorimetric evaluation of protective treatments (consolidants) applied to traditional Islamic plasterworks, under natural ageing conditions. From analyses of the original pictorial plaster remains in the Courtyard of the Maidens of the Real Alcázar in Seville, Spain (a World Heritage Site) we prepared test specimens, using materials and techniques similar to the original ones. We analysed 56 test specimens painted with four pigments (yellow, green, blue, and red), using two different binders (animal glue and gum arabic), onto which five representative consolidants were applied: barium hydroxide, acrylic copolymer, polyvinyl butyral, ethyl silicate, and bacterial carbonatogenesis. The test specimens were subjected to natural ageing for one year (indoors and outdoors), enabling a colorimetric assessment to be made of the changes of the polychrome surfaces. The colorimetric heterogeneity of the 56 specimens after ageing registered an average value of 2.7 CIELAB units, assessed using the mean colour difference with respect to the mean. In the aged specimens, the addition of consolidants resulted in average colour differences (mainly lightness differences) of 10.7 and 6.7 CIELAB units, considering as a reference the specimens without consolidants aged indoors and outdoors, respectively. These colour differences were very similar for both binders but not for the four pigments, higher values being found for the blue and red pigments. Considering as reference the samples without consolidants aged outdoors, we found no statistically significant colour differences, either among the five consolidants (p?=?.094) nor the two binders (p?=?.674) used. In addition to the magnitude of colour differences, the choice of the most appropriate consolidants must also consider aspects related to performance and effectiveness. Overall, for the type of paints tested, the polyvinyl butyral consolidant appeared to perform the best, followed by the ethyl silicate. 相似文献
424.
Manuel Goyanes 《Journalism Practice》2020,14(6):714-729
ABSTRACT The online environment has radically changed the way in which users consume, discover and manipulate news. The growing relevance of social media platforms and digital intermediaries for news sharing and consumption increase the likelihood of citizens to be exposed to online news even when they are not seeking it. This digital transformation fundamentally challenges the way online news use and exposure have been conceptualized and measured, affecting also to citizens’ knowledge about public affairs and politics. This article examines the factors that predict the probability to be an “incidentally exposed news user” online. Specifically, based on a representative US sample from the Pew Research Centre, this study analyses the role of media preference, use and trust. Findings indicate that beyond users’ demographics and loyalty, readers’ news preferences, uses and trust, specially of social media platforms, affect their probability to be incidentally exposed to news online. These results have important empirical and theoretical implications for understanding the connection between readers’ news consumption patterns and online exposure, intentional or incidental. 相似文献
425.
426.
Joan Manuel Marquès Puig Thanasis Daradoumis Laura Calvet Liñan Marta Arguedas 《British journal of educational technology : journal of the Council for Educational Technology》2020,51(1):53-70
This work presents an automated assessment tool for online distributed programming, called DSLab. It is a web-based environment that provides a transparent deployment and execution of assignments in remote computers, a transparent initialization and the possibility to add new logs by the students. DSLab has been evaluated in a real distributed learning environment by analysing students' own perceptions on their learning improvement and exploring whether students' interactions with the tool yielded a fruitful learning experience. Current research provides no evidence that the effects of such a tool have been investigated in the field of online distributed programming. Two types of analysis were performed: a quantitative analysis of data coming from students answering a questionnaire and an analysis of the log files of students' interactions with the tool. Our results showed that students perceived substantial learning improvement from the use of the automated assessment tool. Moreover, students produced fruitful interaction with the tool as soon as they achieved high familiarization and constant activity with it, which ultimately helped them improve their academic performance. Finally, the limitations of the current study and directions for future research are presented. 相似文献
427.
Clinical information seeking in traumatic brain injury: a survey of Veterans Health Administration polytrauma care team members 下载免费PDF全文
428.
In the education world, it is widely accepted that language learning is one of the pioneering disciplines in the application and use of the information and communication technologies, initially preceded by the widespread use of audiovisual resources which, finally integrated in the digital space, bring about the use of multimedia. Additionally, language learning has greatly contributed to standardizing lesson plans. They set the basis for learning design, one of the last advances in the pedagogical organization of education through the use of computers in learning managing systems. Most recently, language learning has also played an innovative role in the implementation of portfolios to education. These three perspectives address different issues for e-learning: the access to content through technology; the design of the learning initially with the help of tutors and then applying institutional frameworks for their own personal learning processes; and the edition of the resulting e-learning experience as evidence with the e-dossier, a part of the e-portfolio. Currently, the web is evolving towards its original function as the read/write web where the user, apart from accessing information, can create their own information and communicate more interactively, using technology-enhanced learning environments to integrate text and multimedia for real audiences both in the classroom and outside. Thus, taking language learning guided by the Common European Framework of Reference for Languages, the authors present here a complete e-learning system which integrates textual as well as multimedia facilities to acquire learning content, edit learning design and report learning experiences. 相似文献
429.
Diego Arroyuelo Veronica Gil-Costa Senén González Mauricio Marin Mauricio Oyarzún 《Information processing & management》2012
Query response times within a fraction of a second in Web search engines are feasible due to the use of indexing and caching techniques, which are devised for large text collections partitioned and replicated into a set of distributed-memory processors. This paper proposes an alternative query processing method for this setting, which is based on a combination of self-indexed compressed text and posting lists caching. We show that a text self-index (i.e., an index that compresses the text and is able to extract arbitrary parts of it) can be competitive with an inverted index if we consider the whole query process, which includes index decompression, ranking and snippet extraction time. The advantage is that within the space of the compressed document collection, one can carry out the posting lists generation, document ranking and snippet extraction. This significantly reduces the total number of processors involved in the solution of queries. Alternatively, for the same amount of hardware, the performance of the proposed strategy is better than that of the classical approach based on treating inverted indexes and corresponding documents as two separate entities in terms of processors and memory space. 相似文献
430.