Investigating married adults’ communal coping with genetic health risk and perceived discrimination

Increased genetic testing in personalized medicine presents unique challenges for couples, including managing disease risk and potential discrimination as a couple. This study investigated couples’ conflicts and support gaps as they coped with perceived genetic discrimination. We also explored the degree to which communal coping was beneficial in reducing support gaps and ultimately stress. Dyadic analysis of married adults (N?=?266, 133 couples), in which one person had the genetic risk for serious illness, showed that perceived discrimination predicted more frequent conflicts about alpha-1 antitrypsin deficiency-related treatment, privacy boundaries, and finances, which, in turn, predicted wider gaps in emotion and esteem support, and greater stress for both spouses. Communal coping predicted lower support gaps for both partners and marginally lower stress.     

The Role of Consistency in Detecting Deception: The Superiority of Correspondence over Coherence

Inconsistency is often considered an indication of deceit. The conceptualization of consistency used in deception research, however, has not made a clear distinction between two concepts long differentiated by philosophers: coherence and correspondence. The existing literature suggests that coherence is not generally useful for deception detection. Correspondence, however, appears to be quite useful. The present research developed a model of how correspondence is utilized to make judgments, and this article reports on four studies designed to elaborate on the model. The results suggest that judges attend strongly to correspondence and that they do so in an additive fashion. As noncorrespondent information accumulates, an increasingly smaller proportion of judges make truthful assessments of guilty suspects. This work provides a basic framework for examining how information is utilized to make deception judgments and forms the correspondence and coherence module of truth-default theory.     

Applying LDA Topic Modeling in Communication Research: Toward a Valid and Reliable Methodology

ABSTRACT

Latent Dirichlet allocation (LDA) topic models are increasingly being used in communication research. Yet, questions regarding reliability and validity of the approach have received little attention thus far. In applying LDA to textual data, researchers need to tackle at least four major challenges that affect these criteria: (a) appropriate pre-processing of the text collection; (b) adequate selection of model parameters, including the number of topics to be generated; (c) evaluation of the model’s reliability; and (d) the process of validly interpreting the resulting topics. We review the research literature dealing with these questions and propose a methodology that approaches these challenges. Our overall goal is to make LDA topic modeling more accessible to communication researchers and to ensure compliance with disciplinary standards. Consequently, we develop a brief hands-on user guide for applying LDA topic modeling. We demonstrate the value of our approach with empirical data from an ongoing research project.     

Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.