Coupling tendencies during exploratory behaviours of competing players in rugby union dyads

This study investigated interpersonal coordination tendencies in 1vs.1 dyads in rugby union, here expressed by participants' movement velocity towards or away from the sideline as they competed to score or prevent a try. We examined whether coupling tendencies of members of each dyad shaped key performance outcomes (try or successful tackle). Data on movement displacement trajectories of eight male rugby union players (aged 11–12 years) were analysed during performance in 47 trials. To assess coordination tendencies during exploratory behaviours in the dyads, analyses of performance time series data were undertaken using variable time graphs, running correlations and cross-correlations. Results revealed distinct coupling patterns characterised by shifts between synchronous coordination and asynchronous coordination tendencies and uncoordinated actions. Observed behaviours were interpreted as attempts of competing participants to create and perceive possibilities for action while seeking to achieve specific performance goals. Findings also revealed that a variety of patterned relations between participants resulted in different performance outcomes.     

Impact of an incremental running test on jumping kinematics in endurance runners: can jumping kinematic explain the post-activation potentiation phenomenon?

This study aimed to determine whether kinematic data during countermovement jump (CMJ) might explain post-activation potentiation (PAP) phenomenon after an exhausting running test. Thirty-three trained endurance runners performed the Léger Test; an incremental test which consists of continuous running between two lines 20 m apart. CMJ performance was determined before (pre-test) and immediately after the protocol (post-test). Sagittal plane, video of CMJs was recorded and kinematic data were obtained throughout 2-Dimensional analysis. In addition to the duration of eccentric and concentric phases of CMJ, hip, knee and ankle angles were measured at four key points during CMJ: the lowest position of the squat, take-off, landing, and at the lowest position after landing. Additionally, heart rate was monitored, and rate of perceived exertion was recorded at post-test. Analysis of variance revealed a significant improvement in CMJ (p = 0.002) at post-test. Cluster analysis grouped according to whether PAP was experienced (responders group: RG, n = 25) or not (non-responders group: NRG, n = 8) relative to CMJ change from rest to post-test. RG significantly improved (p < 0.001) the performance in CMJ, whereas NRG remained unchanged. Kinematic data did not show significant differences between RG and NRG. Thus, the data suggest that jumping kinematic does not provide the necessary information to explain PAP phenomenon after intensive running exercises in endurance athletes.     

Movement velocity as a measure of exercise intensity in three lower limb exercises

The purpose of this study was to investigate the relationship between movement velocity and relative load in three lower limbs exercises commonly used to develop strength: leg press, full squat and half squat. The percentage of one repetition maximum (%1RM) has typically been used as the main parameter to control resistance training; however, more recent research has proposed movement velocity as an alternative. Fifteen participants performed a load progression with a range of loads until they reached their 1RM. Maximum instantaneous velocity (V_max) and mean propulsive velocity (MPV) of the knee extension phase of each exercise were assessed. For all exercises, a strong relationship between V_max and the %1RM was found: leg press (r²_adj = 0.96; 95% CI for slope is [?0.0244, ?0.0258], P < 0.0001), full squat (r²_adj = 0.94; 95% CI for slope is [?0.0144, ?0.0139], P < 0.0001) and half squat (r²_adj = 0.97; 95% CI for slope is [?0.0135, ?0.00143], P < 0.0001); for MPV, leg press (r²_adj = 0.96; 95% CI for slope is [?0.0169, ?0.0175], P < 0.0001, full squat (r²_adj = 0.95; 95% CI for slope is [?0.0136, ?0.0128], P < 0.0001) and half squat (r²_adj = 0.96; 95% CI for slope is [?0.0116, 0.0124], P < 0.0001). The 1RM was attained with a MPV and V_max of 0.21 ± 0.06 m s^?1 and 0.63 ± 0.15 m s^?1, 0.29 ± 0.05 m s^?1 and 0.89 ± 0.17 m s^?1, 0.33 ± 0.05 m s^?1 and 0.95 ± 0.13 m s^?1 for leg press, full squat and half squat, respectively. Results indicate that it is possible to determine an exercise-specific %1RM by measuring movement velocity for that exercise.     

Automatic evaluation of digital libraries with 5SQual

Digital libraries (DLs) are complex information systems which can present changes in their structure, content, and services. These complexities and dynamics make system maintenance a non-trivial task, since it requires periodical evaluation of the different DL components. Generally, these evaluations are customized per system and are performed only when problems occur and administrator intervention is required. This work aims to change the situation. We present 5SQual, a tool which provides ways to perform automatic and configurable evaluations of some of the most important DL components, among them, digital objects, metadata, and services. The tool implements diverse numeric indicators that are associated with eight quality dimensions described in the 5S quality model. Its generic architecture was developed to be applicable to various DLs and scenarios. In sum, the main contributions of this work include: (i) the design and implementation of 5SQual, a tool that validates a theoretical DL quality model; (ii) the demonstration of the applicability of the tool in several usage scenarios; and (iii) the evaluation (with usability specialists) of its graphical interface specially designed to guide the configuration of 5SQual evaluations. We also present the results of interviews conducted with administrators of real DLs regarding their expectations and opinions about 5SQual.     

Serial position effects in social transmission of food preference

The serial position effect in Long-Evans rats was evaluated in two experiments. In Experiment 1, the effect in a group for which an interstimulus interval (ISI) was introduced between items in a list of demonstrators was compared with that in a group without an ISI. With an ISI, a recency effect was produced. In Experiment 2, a serial position effect group was compared with four groups in which either the distinctiveness or the context of the middle item was changed, relative to the items on either side of it. A von Restorff effect was produced when a rat from a different strain was used as a demonstrator in Position 2. The results for Experiment 1 are discussed in relation to interstimulus effects in monkeys and those for Experiment 2 with respect to changes in the physical properties of middle items.     

High-throughput study of alpha-synuclein expression in yeast using microfluidics for control of local cellular microenvironment

Microfluidics is an emerging technology which allows the miniaturization, integration, and automation of fluid handling processes. Microfluidic systems offer low sample consumption, significantly reduced processing time, and the prospect of massive parallelization. A microfluidic platform was developed for the control of the soluble cellular microenvironment of Saccharomyces cerevisiae cells, which enabled high-throughput monitoring of the controlled expression of alpha-synuclein (aSyn), a protein involved in Parkinson's disease. Y-shaped structures were fabricated using particle desorption mass spectrometry-based soft-lithography techniques to generate biomolecular gradients along a microchannel. Cell traps integrated along the microchannel allowed the positioning and monitoring of cells in precise locations, where different, well-controlled chemical environments were established. S. cerevisiae cells genetically engineered to encode the fusion protein aSyn-GFP (green fluorescent protein) under the control of GAL1, a galactose inducible promoter, were loaded in the microfluidic structure. A galactose concentration gradient was established in the channel and a time-dependent aSyn-GFP expression was obtained as a function of the positioning of cells along the galactose gradient. Our results demonstrate the applicability of this microfluidic platform to the spatiotemporal control of cellular microenvironment and open a range of possibilities for the study of cellular processes based on single-cell analysis.