Immunoproteomic analysis of secretory proteins ofAspergillus fumigatus with specific IGE immunoreactivity

Allergenic/antigenic proteins are known to induce Type I and Type III hypersensitivity reactions leading to allergic bronchopulmonary aspergillosis (ABPA) in immunocompetent host. The common structural features or intrinsic properties of the allergens/antigens leading to allergenicity in a host are not well understood. In the current report, comparative analysis of proteins on two dimensional gel electrophoresis (2D-3) and specific IgE immunoblots ofA. fumigatus secretory proteins (1^st, 2^nd and 3^rd week culture filtrate proteins) was carried out. We observed a total of 159 proteins in 1^st, 2^nd and 3^rd week culture filtrates ofA. fumigatus. Specific IgE immunoreactivity was observed in 75 proteins with different intensity. Third week culture filtrate showed maximum number of proteins, 142, and specific IgE immunoreactive proteins, 65. MALDI-TOF analysis resulted in putative identification of two allergens as hypothetical protein YBL057c fromSaccharomyces cereviseae and unnamed protein product fromDebaryomyces hansenii (similar to IPF14568 ofCandida albicans). Identification of a repertoire of specific IgE immunoreactive proteins will facilitate the studies on structure-function relationship of these proteins relevant for diagnosis and pathogenesis.     

Patent data as a guide to industrial activity

In this paper the patenting activity of the international pharmaceutical industry in London between 1900 and 1966 is analysed. Although patent data as a measure of industrial activity is not without defects it can be used both as an adjunct to and as a surrogate for more conventional yardsticks such as concentration ratios, sales statistics and so on. The results indicate that patenting activity can be used as both an absolute and relative indicator of an industry's pace of technological change. They help pinpoint the international distribution of pharmaceutical research and development. If analysed by product or process they can aid in the understanding of why an industry's competitive behaviour is or is not primarily one of price rivalry. Finally, they illustrate the continuing role of the individual inventor.     

Effect of <Emphasis Type="SmallCaps">l</Emphasis>-Thyroxine on Micronuclei Frequency in Peripheral Blood Lymphocytes in Clinical and Experimental Conditions

The aim was to study the genotoxic effect of high concentration of thyroxine (T₄) in vivo in peripheral blood lymphocytes (PBL) of the patients suffering from thyroid disorders. The effect was compared by performing in vitro experiments with addition of increasing concentration of T₄ (0.125–1 µM) in whole blood samples from healthy donors. Cytokinesis-blocked micronuclei (CBMN) assay method was used to assess the DNA damage in the PBL. The study included 104 patients which were grouped as control (n = 49), hyperthyroid (n = 31) and hypothyroid (n = 24). A significant increase in micronuclei (MN) frequency was observed in hyperthyroid patients when compared with the hypothyroid and euthyroid group thereby suggesting increased genotoxicity in hyperthyroidism (p < 0.001). A significant increase in MN frequency was observed at T₄ concentration of 0.5 µM and above when compared to lower T₄ concentrations (0.125 and 0.25 µM) and basal in in vitro experiments (p = 0.000). The results indicate that the T₄ in normal concentration does not exhibit the genotoxic effect, as observed in both the in vivo and in vitro experiments. The toxicity of T₄ increases at and above 0.5 μM concentration in vitro. Therefore acute T₄ overdose should be handled promptly and effectively so as to avoid the possible genotoxic effect of high concentration of T₄ in vivo.     

Seaweed uses: the outlook for mariculture

The decade of the 1970s heralded a major research effort into mariculture of economically important red and brown seaweeds. Limited and declining natural populations, lack of synthetic substitutes for the phycocolloids, and their upwardly spiralling costs have emphasized the need for more research in this area. Algal mariculture is now technically and scientifically feasible, but the economic realities are still undetermined. This article examines what is known about seaweed mariculture and discusses where future research efforts must go.     

Technological transitions as evolutionary reconfiguration processes: a multi-level perspective and a case-study

This paper addresses the question of how technological transitions (TT) come about? Are there particular patterns and mechanisms in transition processes? TT are defined as major, long-term technological changes in the way societal functions are fulfilled. TT do not only involve changes in technology, but also changes in user practices, regulation, industrial networks, infrastructure, and symbolic meaning or culture. This paper practices ‘appreciative theory’ [R.R. Nelson, S.G. Winter, An Evolutionary Theory of Economic Change, Bellknap Press, Cambridge, MA, 1982] and brings together insights from evolutionary economics and technology studies. This results in a multi-level perspective on TT where two views of the evolution are combined: (i) evolution as a process of variation, selection and retention, (ii) evolution as a process of unfolding and reconfiguration. The perspective is empirically illustrated with a qualitative longitudinal case-study, the transition from sailing ships to steamships, 1780–1900. Three particular mechanisms in TT are described: niche-cumulation, technological add-on and hybridisation, riding along with market growth.     

Epochē, Entertainment and Ethics: On the Hyperreality of Everyday Life

In this essay, I argue that popular entertainment can be understood in terms of Husserl’s concepts of epochē, reduction and constitution, and, conversely, that epochē, reduction and constitution can be explicated in terms of popular entertainment. To this end I use Husserl’s concepts to explicate and reflect upon the psychological and ethical effects of an exemplary instance of entertainment, the renowned Star Trek episode entitled “The Measure of a Man.” The importance of such an exercise is twofold: (1) to demonstrate, once again, the fecundity of the methodological procedures Husserl bequeathed to us; more than any other philosopher, he tapped into the fundamental manners in which we lose, make and remake the meanings of our lives; and (2) to demonstrate how popular entertainment, similarly, plays a central role in the making and remaking of the meanings of our lives. If my zig-zag procedure between Husserl’s philosophy and popular entertainment is productive and cogent, in addition to elucidating Husserl’s philosophy, it will demonstrate the reality-generating potency and the constitutive power of entertainment in the contemporary world. Entertainment, via ourselves, has become the primary producer of the meanings via which consciousness constitutes the world.     

Domesticating Computers and the Internet

The people who use computers and the ways they use them have changed substantially over the past 25 years. In the beginning highly educated people, mostly men, in technical professions used computers for work, but over time a much broader range of people are using computers for personal and domestic purposes. This trend is still continuing, and over a shorter time scale has been replicated with the use of the Internet. This paper uses data from four national surveys to document how personal computers and the Internet have become increasingly domesticated since 1995 and to explore the mechanisms for this shift. Now people log on more often from home than from places of employment and do so for pleasure and for personal purposes rather than for their jobs. Analyses comparing veteran Internet users to novices in 1998 and 2000 and analyses comparing the change in use within a single sample between 1995 and 1996 support two complementary explanations for how these technologies have become domesticated. Women, children, and less well-educated individuals are increasingly using computers and the Internet and have a more personal set of motives than well-educated men. In addition, the widespread diffusion of the PC and the Internet and the response of the computing industry to the diversity in consumers has led to a rich set of personal and domestic services.